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Literature summary extracted from

  • Eprintsev, A.T.; Fedorin, D.N.; Sazonova, O.V.; Igamberdiev, A.U.
    Expression and properties of the mitochondrial and cytosolic forms of fumarase in sunflower cotyledons (2018), Plant Physiol. Biochem., 129, 305-309 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 0.0025
-
L-malate isoform Fum2, pH 7.5, temperature not specified in the publication Helianthus annuus
4.2.1.2 0.0125
-
L-malate isoform Fum1, pH 7.5, temperature not specified in the publication Helianthus annuus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.2 cytosol about 25% of total activity Helianthus annuus 5829
-
4.2.1.2 mitochondrion about 75% of total activity Helianthus annuus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.2 Mg2+ 10fold activation of isoform Fum2 Helianthus annuus
4.2.1.2 Mg2+ about 20fold activation of isoform Fum1 Helianthus annuus
4.2.1.2 Mn2+ 15fold activation of isoform Fum2 Helianthus annuus
4.2.1.2 additional information not activating: Ca2+, K+ Helianthus annuus
4.2.1.2 additional information not activating: Ca2+, K+, Mn2+ Helianthus annuus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Helianthus annuus A0A251UH44 isoform Fum1
-
4.2.1.2 Helianthus annuus A0A251UZF1 isoform Fum2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.2 cotyledon
-
Helianthus annuus
-
4.2.1.2 seed expression of the mitochondrial (Fum1) and cytosolic (Fum2) isoforms increases during germination of sunflower seeds with the peak of the Fum1 gene on the second day and of the Fum2 gene on the third day Helianthus annuus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.2 5.43
-
isoform Fum2, pH 7.5, temperature not specified in the publication Helianthus annuus
4.2.1.2 5.97
-
isoform Fum1, pH 7.5, temperature not specified in the publication Helianthus annuus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 L-malate
-
Helianthus annuus fumarate + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.2 FUM1
-
Helianthus annuus
4.2.1.2 FUM2
-
Helianthus annuus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.2 7.5
-
-
Helianthus annuus
4.2.1.2 8
-
-
Helianthus annuus