EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.45 | expressed in Escherichia coli BL21(DE3) cells | Thermoanaerobacterium xylanolyticum |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.45 | hanging drop vapor diffusion method, using 0.2 M ammonium sulfate, 15% (w/v) PEG 6000, 0.1 M Bis-Tris propane, pH 8.0 | Thermoanaerobacterium xylanolyticum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.45 | D508H | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | D508N | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | D593A | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | E441A | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | R544W | the mutant shows slightly increased activity compared to the wild type enzyme | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | R786H | the mutant shows reduced activity compared to the wild type enzyme | Thermoanaerobacterium xylanolyticum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.45 | 2,4-dinitrophenyl 2-deoxy-2-fluoro-beta-D-glucoside | - |
Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | deoxynojirimycin | - |
Thermoanaerobacterium xylanolyticum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.45 | 0.18 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 0.21 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme with N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 0.25 | - |
cellobiose | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 0.26 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme R544W, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 0.27 | - |
laminaribiose | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 15.7 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme R786H, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 16.3 | - |
4-nitrophenyl beta-D-galactoside | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 41 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D508N, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 110 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D508H, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.45 | glucosylceramide + H2O | Thermoanaerobacterium xylanolyticum | - |
beta-D-glucose + ceramide | - |
? | |
3.2.1.45 | glucosylceramide + H2O | Thermoanaerobacterium xylanolyticum GH116 | - |
beta-D-glucose + ceramide | - |
? | |
3.2.1.45 | glucosyllysosphingosine + H2O | Thermoanaerobacterium xylanolyticum | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.45 | Thermoanaerobacterium xylanolyticum | F6BL85 | - |
- |
3.2.1.45 | Thermoanaerobacterium xylanolyticum GH116 | F6BL85 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.45 | Talon cobalt affinity resin column chromatography, HisTrap column chromatography, and Superdex 200 gel filtration | Thermoanaerobacterium xylanolyticum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.45 | 0.0817 | - |
with methyl beta-D-glucoside as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 0.123 | - |
with 4-nitrophenyl N-acetyl-beta-D-glucosaminide as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 2.23 | - |
with gentibiose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 3.58 | - |
with n-octyl beta-D-glucoside as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 3.74 | - |
with n-heptyl beta-D-glucoside as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 4.1 | - |
with sophorose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 8.9 | - |
with 4-nitrophenyl beta-D-galactoside as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 11.7 | - |
with laminaribiose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 13.4 | - |
with cellobiose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 13.5 | - |
with cellohexaose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 13.8 | - |
with cellopentaose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 14.9 | - |
with cellotetraose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 15 | - |
with laminaritetraose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 15.6 | - |
with cellotriose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 16.1 | - |
with laminaritriose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 16.5 | - |
with laminaripentaose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.2.1.45 | 27.3 | - |
with 4-nitrophenyl beta-D-glucoside as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.45 | 4-nitrophenyl beta-cellobioside + H2O | 1.1% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | 4-nitrophenol + cellobiose | - |
? | |
3.2.1.45 | 4-nitrophenyl beta-D-galactoside + H2O | 33% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | 4-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.45 | 4-nitrophenyl beta-D-galactoside + H2O | 33% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum GH116 | 4-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.45 | 4-nitrophenyl beta-D-glucoside + H2O | 100% activity | Thermoanaerobacterium xylanolyticum | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.45 | 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | 0.45% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
3.2.1.45 | cellobiose + H2O | 49% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
3.2.1.45 | cellohexaose + H2O | 49% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
3.2.1.45 | cellotetraose + H2O | 55% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
3.2.1.45 | cellotriose + H2O | 57% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
3.2.1.45 | cellotriose + H2O | 57% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum GH116 | ? | - |
? | |
3.2.1.45 | gentibiose + H2O | 8.2% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
3.2.1.45 | glucosylceramide + H2O | - |
Thermoanaerobacterium xylanolyticum | beta-D-glucose + ceramide | - |
? | |
3.2.1.45 | glucosylceramide + H2O | - |
Thermoanaerobacterium xylanolyticum GH116 | beta-D-glucose + ceramide | - |
? | |
3.2.1.45 | glucosyllysosphingosine + H2O | - |
Thermoanaerobacterium xylanolyticum | ? | - |
? | |
3.2.1.45 | laminaribiose + H2O | 43% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | 2 D-glucose | - |
? | |
3.2.1.45 | laminaripentaose + H2O | 60% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
3.2.1.45 | laminaritetraose + H2O | 55% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
3.2.1.45 | laminaritriose + H2O | 59% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
3.2.1.45 | laminaritriose + H2O | 59% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum GH116 | ? | - |
? | |
3.2.1.45 | methyl beta-D-glucoside + H2O | 0.3% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | methanol + D-glucose | - |
? | |
3.2.1.45 | additional information | the enzyme shows less than 0.04% activity with 4-nitrophenyl alpha-D-galactoside, 4-nitrophenyl alpha-L-arabinoside, 4-nitrophenyl beta-D-fucoside, 4-nitrophenyl alpha-D-glucoside, 4-nitrophenyl beta-D-mannoside, and 4-nitrophenyl beta-D-xyloside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
3.2.1.45 | additional information | the enzyme shows less than 0.04% activity with 4-nitrophenyl alpha-D-galactoside, 4-nitrophenyl alpha-L-arabinoside, 4-nitrophenyl beta-D-fucoside, 4-nitrophenyl alpha-D-glucoside, 4-nitrophenyl beta-D-mannoside, and 4-nitrophenyl beta-D-xyloside | Thermoanaerobacterium xylanolyticum GH116 | ? | - |
? | |
3.2.1.45 | n-heptyl beta-D-glucoside + H2O | 14% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | heptanol + D-glucose | - |
? | |
3.2.1.45 | n-octyl beta-D-glucoside + H2O | 13% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | octanol + D-glucose | - |
? | |
3.2.1.45 | sophorose + H2O | 15% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | 2 D-glucose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.45 | beta-glucosidase | - |
Thermoanaerobacterium xylanolyticum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.45 | 75 | - |
- |
Thermoanaerobacterium xylanolyticum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.45 | 81.3 | - |
melting temperature | Thermoanaerobacterium xylanolyticum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.45 | 3.47 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D508H, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 30.2 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D508N, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 37.6 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme with N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 41.4 | - |
laminaribiose | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 44.4 | - |
cellobiose | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 48.2 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme R544W, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 49 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 193 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme R786H, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 255 | - |
4-nitrophenyl beta-D-galactoside | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.45 | 5.5 | - |
- |
Thermoanaerobacterium xylanolyticum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.45 | 0.031 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D508H, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 0.74 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D508N, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 12.3 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme R786H, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 15.6 | - |
4-nitrophenyl beta-D-galactoside | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 153 | - |
laminaribiose | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 178 | - |
cellobiose | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 179 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme with N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 186 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme R544W, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
3.2.1.45 | 272 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |