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Literature summary extracted from
- Recombinant alpha-L-rhamnosidase from Aspergillus terreus in selective trimming of alpha-L-rhamnose from steviol glycosides (2015), J. Mol. Catal. B, 122, 248-254 .
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.40 | food industry | Aspergillus terreus alpha-L-rhamnosidase specifically hydrolyses the glycosidic linkage of dulcoside A (the bitterest compounds in steviol glycoside mixtures), and converts it to rubusoside. During a 12 h biotransformation, the dulcoside A from crude leaf extracts is completely converted by recombinant alpha-L-rhamnosidase from Aspergillus terreus into rubusoside. This process offers a promising approach for reducing the bitterness of steviol glycoside mixtures | Aspergillus terreus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.40 | expression of alpha-L-rhamnosidase in Pichia pastoris and Kluyveromyces lactis achieves high protein yields of up to 17.6 U/ml and 30.6 U/ml | Aspergillus terreus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.40 | Aspergillus terreus | I0AZ41 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.40 | dulcoside A + H2O | no hydrolytic activity against dulcoside A analogues. No transrhamnosylation activity is observed | Aspergillus terreus | rubusoside + alpha-L-rhamnose | - |
? |  |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.40 | 65 | - |
enzyme produced by Pichia pastoris | Aspergillus terreus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.40 | 60 | - |
half-life: more than 30 h | Aspergillus terreus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.40 | 6 | 8 | recombinant enzymes | Aspergillus terreus |
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Release 2023.1 (January 2023)
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