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Literature summary extracted from

  • Kaushik, V.; Verma, V.V.; Goel, M.
    Functional divergence and comparative in-silico study of Cas4 proteins of DUF83 class (2018), J. Mol. Recognit., 31, e2694 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.12.1 gene Pcal_0546, phylogenetic analysis and tree Pyrobaculum calidifontis
3.1.12.1 gene SSO0001, phylogenetic analysis and tree Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.12.1 Pyrobaculum calidifontis A3MTK6
-
-
3.1.12.1 Pyrobaculum calidifontis VA1 A3MTK6
-
-
3.1.12.1 Saccharolobus solfataricus Q97TX9 i.e. Sulfolobus solfataricus
-
3.1.12.1 Saccharolobus solfataricus ATCC 35092 Q97TX9 i.e. Sulfolobus solfataricus
-
3.1.12.1 Saccharolobus solfataricus DSM 1617 Q97TX9 i.e. Sulfolobus solfataricus
-
3.1.12.1 Saccharolobus solfataricus JCM 11322 Q97TX9 i.e. Sulfolobus solfataricus
-
3.1.12.1 Saccharolobus solfataricus P2 Q97TX9 i.e. Sulfolobus solfataricus
-

Synonyms

EC Number Synonyms Comment Organism
3.1.12.1 Cas4
-
 Saccharolobus solfataricus
3.1.12.1 Cas4
-
 Pyrobaculum calidifontis
3.1.12.1 CRISPR-associated exonuclease Cas4
-
 Saccharolobus solfataricus
3.1.12.1 CRISPR-associated exonuclease Cas4
-
 Pyrobaculum calidifontis
3.1.12.1 DUF83Pc
-
 Pyrobaculum calidifontis
3.1.12.1 DUF83Ss
-
 Saccharolobus solfataricus
3.1.12.1 Pcal_0546
-
 Pyrobaculum calidifontis
3.1.12.1 SSO0001
-
 Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
3.1.12.1 evolution Cas4 proteins exhibit low sequence similarity among themselves and are currently classified into 2 main classes: DUF83 and DUF911. The genome of Sulfolobus solfataricus encodes five Cas4 like protein comprising low sequence similarity (below 30%). Out of them, SSO0001, SSO1392, and SSO1449 belong to DUF83 class, and SSO1391 and SSO1451 belong to DUF911 class. SSO1391 has been reported to show broad range cleavage specificity for cleaving ssDNA in both the directions (5'-3') and (3'-5') (DUF911). By contrast, members of DUF83 class cleave the ssDNA substrate only in the direction 5'-3'. The overall three-dimensional structural fold of DUF83Ss and DUF83Pc appears to be conserved. The separation of Cas4 proteins of DUF83 class into 2 separate branches hints at the possibility of specific sites which guide the evolutionary divergence between Cas4 proteins of the 2 clades, prediction of discriminative motifs among the two subclasses of DUF83 Cas4 proteins through motif analysis, overview. Divergence type I analyses of two clusters and their intra molecular hydrogen bonding with other residues Saccharolobus solfataricus
3.1.12.1 evolution Cas4 proteins exhibit low sequence similarity among themselves and are currently classified into 2 main classes: DUF83 and DUF911. The genome of Sulfolobus solfataricus encodes five Cas4 like protein comprising low sequence similarity (below 30%). Out of them, SSO0001, SSO1392, and SSO1449 belong to DUF83 class, and SSO1391 and SSO1451 belong to DUF911 class. SSO1391 has been reported to show broad range cleavage specificity for cleaving ssDNA in both the directions (5'-3') and (3'-5') (DUF911). By contrast, members of DUF83 class cleave the ssDNA substrate only in the direction 5'-3'. The overall three-dimensional structural fold of DUF83Ss and DUF83Pc appears to be conserved. The separation of Cas4 proteins of DUF83 class into 2 separate branches hints at the possibility of specific sites which guide the evolutionary divergence between Cas4 proteins of the 2 clades, prediction of discriminative motifs among the two subclasses of DUF83 Cas4 proteins through motif analysis, overview. Divergence type I analyses of two clusters and their intra molecular hydrogen bonding with other residues Pyrobaculum calidifontis
3.1.12.1 physiological function the Cas4 proteins of the CRISPR-Cas systems are thought to be responsible for the adaptation step in the CRISPR (clustered regularly interspaced short palindromic repeats) mechanism Saccharolobus solfataricus
3.1.12.1 physiological function the Cas4 proteins of the CRISPR-Cas systems are thought to be responsible for the adaptation step in the CRISPR (clustered regularly interspaced short palindromic repeats) mechanism Pyrobaculum calidifontis