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Literature summary extracted from

  • Yang, Y.; Zhu, N.; Yang, J.; Lin, Y.; Liu, J.; Wang, R.; Wang, F.; Yuan, H.
    A novel bifunctional acetyl xylan esterase/arabinofuranosidase from Penicillium chrysogenum P33 enhances enzymatic hydrolysis of lignocellulose (2017), Microb. Cell Fact., 16, 166 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.1.72 degradation enzyme displays significant synergy with a xylanase, with a degree of synergy of 1.35 for the hydrolysis of delignified corn stover. Release of glucose is increased by 51% from delignified corn stover when 2 mg of a commercial cellulase is replaced by an equivalent amount of Axe Penicillium chrysogenum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 expression in Pichia pastoris Penicillium chrysogenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.72 0.465
-
 4-nitrophenyl acetate pH 7.0, 40'°C Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Penicillium chrysogenum A0A2H4V677
-
-
3.1.1.72 Penicillium chrysogenum P33 A0A2H4V677
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.72 recombinant protein Penicillium chrysogenum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.72 4.8
-
 substrate 4-nitrophenyl acetate, pH 7.0, 40°C Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Penicillium chrysogenum 4-nitrophenol + acetate
-
 ?
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Penicillium chrysogenum P33 4-nitrophenol + acetate
-
 ?
3.1.1.72 additional information no substrates: 4-hydroxy-3-methoxycinnamic acid methyl ester or ethyl-4-hydroxy-3-methoxycinnamate Penicillium chrysogenum ?
-
 ?
3.1.1.72 additional information no substrates: 4-hydroxy-3-methoxycinnamic acid methyl ester or ethyl-4-hydroxy-3-methoxycinnamate Penicillium chrysogenum P33 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.72 ? x * 31160, SDS-PAGE Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 40
-
-
 Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.72 7
-
-
 Penicillium chrysogenum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.72 4 6 1 h, no loss of activity Penicillium chrysogenum

General Information

EC Number General Information Comment Organism
3.1.1.72 physiological function sequence reveals a GH62 domain exhibiting alpha-L-arabinofuranosidase activity, in addition to the expected esterase domain Penicillium chrysogenum