EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.1 | DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Paenibacillus sp. SSG-1 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.1 | 2-mercaptoethanol | 85.9% inhibition at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | Co2+ | 75.9% inhibition at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | Cu2+ | 64.1% inhibition at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | DTT | 20.1% inhibition at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | EDTA | 81.9% inhibition at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | Fe2+ | 55.3% inhibition at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | Li+ | 26.2% inhibition at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | Mn2+ | 49.1% inhibition at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | Ni2+ | 65.9% inhibition at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | Pb2+ | 80.6% inhibition at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | PMSF | 21.2% inhibition at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | SDS | 71.0% inhibition at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | Urea | 58.5% inhibition at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | Zn2+ | 80.5% inhibition at 1 mM | Paenibacillus sp. SSG-1 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | 0.13 | - |
soluble starch | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 1.42 | - |
maltoheptaose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 1.92 | - |
maltopentaose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 2.28 | - |
maltotriose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 2.86 | - |
maltose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 3.25 | - |
amylose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.1 | cytoplasm | - |
Paenibacillus sp. SSG-1 | 5737 | - |
3.2.1.1 | intracellular | - |
Paenibacillus sp. SSG-1 | 5622 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.1 | Ba2+ | activates 7.4% at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | Ca2+ | activates 28.2% at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | Mg2+ | activates 6.9% at 1 mM | Paenibacillus sp. SSG-1 | |
3.2.1.1 | additional information | poor effects by 1 mM of K+ and Na+ | Paenibacillus sp. SSG-1 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.1 | Paenibacillus sp. SSG-1 | A0A0U4VEK2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.1 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Paenibacillus sp. SSG-1 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 122.04 | - |
purified recombinant enzyme, pH 6.5, 40°C, substrate amylose | Paenibacillus sp. SSG-1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | amylopectin + H2O | 71.1% activity compared to amylose | Paenibacillus sp. SSG-1 | D-glucose + maltose | - |
? | |
3.2.1.1 | amylose + H2O | best substrate | Paenibacillus sp. SSG-1 | D-glucose + maltose | - |
? | |
3.2.1.1 | beta-cyclodextrin + H2O | 39.0% activity compared to amylose | Paenibacillus sp. SSG-1 | D-glucose + maltose | - |
? | |
3.2.1.1 | glycogen + H2O | 40.2% activity compared to amylose | Paenibacillus sp. SSG-1 | D-glucose + maltose | - |
? | |
3.2.1.1 | maltoheptaose + H2O | - |
Paenibacillus sp. SSG-1 | D-glucose + maltose + ? | - |
? | |
3.2.1.1 | maltopentaose + H2O | - |
Paenibacillus sp. SSG-1 | D-glucose + maltose + ? | - |
? | |
3.2.1.1 | maltose + H2O | - |
Paenibacillus sp. SSG-1 | 2 D-glucose | - |
? | |
3.2.1.1 | maltotriose + H2O | - |
Paenibacillus sp. SSG-1 | D-glucose + maltose | - |
? | |
3.2.1.1 | additional information | the enzyme Amy can hydrolyze typical substrates into glucose and maltose, substrate specificity, overview. Enzyme Amy broad substrate range and transglycosylation activity when compared with intracellular alpha-amylase AmyB. Amy exhibits transglycosylation activity when reacted with maltose and maltotriose | Paenibacillus sp. SSG-1 | ? | - |
? | |
3.2.1.1 | pullulan + H2O | 54.4% activity compared to amylose | Paenibacillus sp. SSG-1 | D-glucose + maltose | - |
? | |
3.2.1.1 | soluble starch + H2O | 93.3% activity compared to amylose | Paenibacillus sp. SSG-1 | D-glucose + maltose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.1 | ? | x * 71700, recombinant His-tagged enzyme, SDS-PAGE | Paenibacillus sp. SSG-1 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.1 | Amy | - |
Paenibacillus sp. SSG-1 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 35 | 45 | recombinant His-tagged enzyme | Paenibacillus sp. SSG-1 |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | - |
55 | activity range, profile overview | Paenibacillus sp. SSG-1 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | - |
40 | purified recombinant His-tagged enzyme, over 80% activity remaining | Paenibacillus sp. SSG-1 |
3.2.1.1 | 50 | - |
purified recombinant His-tagged enzyme, 60% activity remaining | Paenibacillus sp. SSG-1 |
3.2.1.1 | 60 | - |
purified recombinant His-tagged enzyme, 10% activity remaining | Paenibacillus sp. SSG-1 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | 0.72 | - |
soluble starch | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 9.21 | - |
amylose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 428.98 | - |
maltose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 538.95 | - |
maltotriose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 567.01 | - |
maltopentaose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 573.8 | - |
maltoheptaose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 6.5 | - |
recombinant His-tagged enzyme | Paenibacillus sp. SSG-1 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 5.6 | 8 | 50% of maximal activity of recombinant His-tagged enzyme within this range, inactive below pH 4.5 | Paenibacillus sp. SSG-1 |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 5 | - |
purified recombinant His-tagged enzyme, 30% activity remaining | Paenibacillus sp. SSG-1 |
3.2.1.1 | 5.5 | 7.5 | purified recombinant His-tagged enzyme, 80% activity remaining | Paenibacillus sp. SSG-1 |
3.2.1.1 | 9 | - |
purified recombinant His-tagged enzyme, 30% activity remaining | Paenibacillus sp. SSG-1 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.1 | evolution | enzyme Amy from Paenibacillus sp. SSG-1 exhibits unexpectedly sequence similarity and evolutionary relationships with alpha-glucanotransferase, homology modelling, using the crystal structure of a secretory alpha-amylase AmyA (PDB ID: 1WZA) as the template, and phylogenetic analysis | Paenibacillus sp. SSG-1 |
3.2.1.1 | additional information | docking study of the enzyme with maltose | Paenibacillus sp. SSG-1 |
3.2.1.1 | physiological function | enzyme Amy may play an important role in utilizing maltooligosaccharides that are released from extracellular alpha-glucan or storage alpha-glucan (glycogen) in Paenibacillus sp. SSG-1 | Paenibacillus sp. SSG-1 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | 2.83 | - |
amylose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 5.41 | - |
soluble starch | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 150.17 | - |
maltose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 236.38 | - |
maltotriose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 295.32 | - |
maltopentaose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 | |
3.2.1.1 | 405.03 | - |
maltoheptaose | pH 6.5, 40°C, recombinant enzyme | Paenibacillus sp. SSG-1 |