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Literature summary extracted from

  • Remy, B.; Plener, L.; Poirier, L.; Elias, M.; Daude, D.; Chabriere, E.
    Harnessing hyperthermostable lactonase from Sulfolobus solfataricus for biotechnological applications (2016), Sci. Rep., 6, 37780 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.81 Methylcyclohexane about 250% activity at 100% (v/v) Saccharolobus solfataricus
3.1.8.1 Methylcyclohexane about 250% activity at 100% (v/v) Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 expressed in Escherichia coli BL21(DE3) cells Saccharolobus solfataricus
3.1.8.1 expressed in Escherichia coli BL21(DE3) cells Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.81 W263I the mutant has increased lactonase and phosphotriesterase activities compared to the wild type Saccharolobus solfataricus
3.1.8.1 W263I the mutant has increased lactonase and phosphotriesterase activities compared to the wild type Saccharolobus solfataricus

General Stability

EC Number General Stability Organism
3.1.1.81 with the liquid enzyme, the remaining activity decreases down to 40% at the highest dose of beta-radiation (100 kGy) Saccharolobus solfataricus
3.1.8.1 with the liquid enzyme, the remaining activity decreases down to 40% at the highest dose of beta-radiation (100 kGy) Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.81 petroleum ether petroleum ether slightly reduces the activity down to 78% Saccharolobus solfataricus
3.1.1.81 Toluene toluene slightly reduced the activity down to 74% Saccharolobus solfataricus
3.1.1.81 xylene xylene decreases the enzyme activity by more than 75% Saccharolobus solfataricus
3.1.8.1 petroleum ether petroleum ether slightly reduces the activity down to 78% Saccharolobus solfataricus
3.1.8.1 Toluene toluene slightly reduces the activity down to 74% Saccharolobus solfataricus
3.1.8.1 xylene xylene decreases the enzyme activity by more than 75% Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.81 an N-acyl-L-homoserine lactone + H2O Saccharolobus solfataricus
-
 an N-acyl-L-homoserine
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.81 additional information the enzyme activity is not affected by acetone, acetonitrile, butanone, butyl acetate, chloroform, dichloromethane, diethyl ether, ethanol, ethyl acetate, isopropanol, methanol, and methoxypropanol Saccharolobus solfataricus
3.1.8.1 additional information the enzyme activity is not affected by acetone, acetonitrile, butanone, butyl acetate, chloroform, dichloromethane, diethyl ether, ethanol, ethyl acetate, isopropanol, methanol, and methoxypropanol Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Saccharolobus solfataricus
-
 Sulfolobus solfataricus
-
3.1.8.1 Saccharolobus solfataricus
-
 Sulfolobus solfataricus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 an N-acyl-L-homoserine lactone + H2O
-
 Saccharolobus solfataricus an N-acyl-L-homoserine
-
 ?
3.1.1.81 ethyl paraoxon + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + diethyl phosphate
-
 ?
3.1.8.1 ethyl paraoxon + H2O
-
 Saccharolobus solfataricus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 POX
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.81 additional information
-
 106°C is the melting temperature. The temperatures required to lose one half of enzymatic activity in 5 minutes are 92°C and 130°C in liquid and solid states respectively. More than 30% activity remains after autoclaving the enzyme powder at 121°C for 15 min Saccharolobus solfataricus
3.1.8.1 additional information
-
 106°C is the melting temperature. The temperatures required to lose one half of enzymatic activity in 5 minutes are 92°C and 130°C in liquid and solid states, respectively. More than 30% activity remains after autoclaving the enzyme powder at 121°C for 15 min Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
3.1.1.81 metabolism the enzyme also exhibits promiscuous phosphotriesterase activity for the degradation of organophosphorous chemicals including insecticides and chemical warfare agents Saccharolobus solfataricus
3.1.8.1 metabolism the enzyme exhibits promiscuous phosphotriesterase activity for the degradation of organophosphorous chemicals including insecticides and chemical warfare agents Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.8.1 1.2
-
 ethyl paraoxon at pH 8.0 and 23°C Saccharolobus solfataricus
3.1.8.1 1.4
-
 ethyl paraoxon with 50% (v/v) glycerol, at pH 8.0 and -18°C Saccharolobus solfataricus
3.1.8.1 25
-
 ethyl paraoxon at pH 8.0 and 70°C Saccharolobus solfataricus