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Literature summary extracted from

  • Homaei, A.; Stevanato, R.; Etemadipour, R.; Hemmati, R.
    Purification, catalytic, kinetic and thermodynamic characteristics of a novel ficin from Ficus johannis (2017), Biocatal. Agricult. Biotechnol., 10, 360-366 .
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.3 0.0137
-
 casein pH 7.0, 60°C Ficus johannis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.3 BaCl2 5 mM, 9% inhibition Ficus johannis
3.4.22.3 CaCl2 5 mM, 31% inhibition Ficus johannis
3.4.22.3 CuCl2 5 mM, complete inhibition Ficus johannis
3.4.22.3 HgCl2 5 mM, complete inhibition Ficus johannis
3.4.22.3 MgCl2 5 mM, 37% inhibition Ficus johannis
3.4.22.3 NiCl2 5 mM, 88% inhibition Ficus johannis
3.4.22.3 PbCl2 5 mM, complete inhibition Ficus johannis
3.4.22.3 ZnCl2 5 mM, 74% inhibition Ficus johannis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.3 25000
-
 SDS-PAGE Ficus johannis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.3 Ficus johannis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.3
-
 Ficus johannis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.3 latex
-
 Ficus johannis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.3 casein + H2O
-
 Ficus johannis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.3 ? x * 25000, SDS-PAGE Ficus johannis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.3 60
-
-
 Ficus johannis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.3 30 80 30°C: about 50% of maximal activity, 80°C: about 60% of maximal activity Ficus johannis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.3 32.9
-
 casein pH 7.0, 60°C Ficus johannis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.3 7
-
-
 Ficus johannis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.3 4 9 pH 4.0: about 55% of maximal activity, pH 9.0: about 70% of maximal activity Ficus johannis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.22.3 2401
-
 casein pH 7.0, 60°C Ficus johannis