EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.174 | additional information | EDTA has no significant effect on enzyme activity | Penicillium chrysogenum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.174 | gene Pcrgrh78A, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression in Aspergillus oryzae strain niaD300, subcloning in Escherichia coli strain DH5alpha | Penicillium chrysogenum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.174 | Ag+ | - |
Penicillium chrysogenum | |
3.2.1.174 | Cu2+ | - |
Penicillium chrysogenum | |
3.2.1.174 | Fe3+ | - |
Penicillium chrysogenum | |
3.2.1.174 | Hg2+ | - |
Penicillium chrysogenum | |
3.2.1.174 | L-rhamnose | 50% inhibition at 50 mM | Penicillium chrysogenum | |
3.2.1.174 | additional information | EDTA has no significant effect on enzyme activity | Penicillium chrysogenum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.174 | extracellular | - |
Penicillium chrysogenum | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.174 | additional information | Ba2+, Ca2+, Cd2+, Co2+, Fe2+, K+, Mn2+, Na+, Ni2+, and Zn2+ have no significant effect on the enzyme activity | Penicillium chrysogenum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.174 | 96000 | - |
recombinant enzyme, gel filtration | Penicillium chrysogenum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.174 | Penicillium chrysogenum | A0A110AZ24 | i.e. Penicillium notatum | - |
3.2.1.174 | Penicillium chrysogenum 31B | A0A110AZ24 | i.e. Penicillium notatum | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.174 | glycoprotein | the native extracellular enzyme is N-linked glycosylated, deglycosylation with endoglycosidase H. Fifteen asparagine residues in PcR-GRH78A are identified as possible N-glycosylation sites | Penicillium chrysogenum |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.174 | native extracellular enzyme from Penicillium chrysogenum strain 31B and recombinant enzyme expressed in Aspergillus oryzae strain niaD300 both about 10fold by ultrafiltration, ammonium sulfate fractionation, anion exchange chromatography, and two different steps of hydrophobic interaction chromatography, followed by dialysis and gel filtration | Penicillium chrysogenum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.174 | 39.6 | - |
purified recombinant enzyme, pH 5.0, 37°C | Penicillium chrysogenum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.174 | additional information | enzyme PcRGRH78A specifically hydrolyzes rhamnogalacturonan oligosaccharides that contain rhamnose at their nonreducing ends, releasing the L-rhamnose, but it has no activity toward naringin, hesperidin,or rutin. When galactosyl rhamnogalacturonan oligosaccharides are used as substrate, PcRGRH78A releases Rha in 3.5fold greater amounts in the presence of beta-galactosidase than in its absence, indicating that PcRGRH78A preferentially acts on Rha residues without the galactose moietyat nonreducing ends | Penicillium chrysogenum | ? | - |
? | |
3.2.1.174 | additional information | enzyme PcRGRH78A specifically hydrolyzes rhamnogalacturonan oligosaccharides that contain rhamnose at their nonreducing ends, releasing the L-rhamnose, but it has no activity toward naringin, hesperidin,or rutin. When galactosyl rhamnogalacturonan oligosaccharides are used as substrate, PcRGRH78A releases Rha in 3.5fold greater amounts in the presence of beta-galactosidase than in its absence, indicating that PcRGRH78A preferentially acts on Rha residues without the galactose moietyat nonreducing ends | Penicillium chrysogenum 31B | ? | - |
? | |
3.2.1.174 | rhamnogalacturonan oligosaccharide + H2O | two oligosaccharides, RG4 (Rha-GalA-Rha-GalA: RG4) and the RG hexasaccharide (Rha-GalA-Rha-GalA-Rha-GalA: RG6), prepared from potato rhamnogalacturonan I | Penicillium chrysogenum | L-rhamnose + ? | - |
? | |
3.2.1.174 | rhamnogalacturonan oligosaccharide + H2O | two oligosaccharides, RG4 (Rha-GalA-Rha-GalA: RG4) and the RG hexasaccharide (Rha-GalA-Rha-GalA-Rha-GalA: RG6), prepared from potato rhamnogalacturonan I | Penicillium chrysogenum 31B | L-rhamnose + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.174 | monomer | 1 * 82000, glycosylated enzyme, SDS-PAGE, 1 * 67000, deglycosylated enzyme, SDS-PAGE, 1 * 101000, recombinant enzyme, SDS-PAGE | Penicillium chrysogenum |
3.2.1.174 | More | peptide mapping | Penicillium chrysogenum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.174 | alpha-L-rhamnosidase | - |
Penicillium chrysogenum |
3.2.1.174 | PcRGRH78A | - |
Penicillium chrysogenum |
3.2.1.174 | RG rhamnohydrolase | - |
Penicillium chrysogenum |
3.2.1.174 | RGRH | - |
Penicillium chrysogenum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.174 | 37 | - |
assay at | Penicillium chrysogenum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.174 | 5 | - |
assay at | Penicillium chrysogenum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.174 | evolution | the enzyme belongs to the glycoside hydrolase family 78, GH78, group 3. Enzyme PcRGRH78A contains a bacterial alpha-L-rhamnosidase (PF05592) domain, but shows no activity with rhamnogalacturonan oligosaccharides | Penicillium chrysogenum |