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Literature summary extracted from

  • Matsumoto, S.; Yamada, H.; Kunishige, Y.; Takenaka, S.; Nakazawa, M.; Ueda, M.; Sakamoto, T.
    Identification of a novel Penicillium chrysogenum rhamnogalacturonan rhamnohydrolase and the first report of a rhamnogalacturonan rhamnohydrolase gene (2017), Enzyme Microb. Technol., 98, 76-85 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.174 additional information EDTA has no significant effect on enzyme activity Penicillium chrysogenum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.174 gene Pcrgrh78A, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression in Aspergillus oryzae strain niaD300, subcloning in Escherichia coli strain DH5alpha Penicillium chrysogenum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.174 Ag+
-
Penicillium chrysogenum
3.2.1.174 Cu2+
-
Penicillium chrysogenum
3.2.1.174 Fe3+
-
Penicillium chrysogenum
3.2.1.174 Hg2+
-
Penicillium chrysogenum
3.2.1.174 L-rhamnose 50% inhibition at 50 mM Penicillium chrysogenum
3.2.1.174 additional information EDTA has no significant effect on enzyme activity Penicillium chrysogenum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.174 extracellular
-
Penicillium chrysogenum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.174 additional information Ba2+, Ca2+, Cd2+, Co2+, Fe2+, K+, Mn2+, Na+, Ni2+, and Zn2+ have no significant effect on the enzyme activity Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.174 96000
-
recombinant enzyme, gel filtration Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.174 Penicillium chrysogenum A0A110AZ24 i.e. Penicillium notatum
-
3.2.1.174 Penicillium chrysogenum 31B A0A110AZ24 i.e. Penicillium notatum
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.174 glycoprotein the native extracellular enzyme is N-linked glycosylated, deglycosylation with endoglycosidase H. Fifteen asparagine residues in PcR-GRH78A are identified as possible N-glycosylation sites Penicillium chrysogenum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.174 native extracellular enzyme from Penicillium chrysogenum strain 31B and recombinant enzyme expressed in Aspergillus oryzae strain niaD300 both about 10fold by ultrafiltration, ammonium sulfate fractionation, anion exchange chromatography, and two different steps of hydrophobic interaction chromatography, followed by dialysis and gel filtration Penicillium chrysogenum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.174 39.6
-
purified recombinant enzyme, pH 5.0, 37°C Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.174 additional information enzyme PcRGRH78A specifically hydrolyzes rhamnogalacturonan oligosaccharides that contain rhamnose at their nonreducing ends, releasing the L-rhamnose, but it has no activity toward naringin, hesperidin,or rutin. When galactosyl rhamnogalacturonan oligosaccharides are used as substrate, PcRGRH78A releases Rha in 3.5fold greater amounts in the presence of beta-galactosidase than in its absence, indicating that PcRGRH78A preferentially acts on Rha residues without the galactose moietyat nonreducing ends Penicillium chrysogenum ?
-
?
3.2.1.174 additional information enzyme PcRGRH78A specifically hydrolyzes rhamnogalacturonan oligosaccharides that contain rhamnose at their nonreducing ends, releasing the L-rhamnose, but it has no activity toward naringin, hesperidin,or rutin. When galactosyl rhamnogalacturonan oligosaccharides are used as substrate, PcRGRH78A releases Rha in 3.5fold greater amounts in the presence of beta-galactosidase than in its absence, indicating that PcRGRH78A preferentially acts on Rha residues without the galactose moietyat nonreducing ends Penicillium chrysogenum 31B ?
-
?
3.2.1.174 rhamnogalacturonan oligosaccharide + H2O two oligosaccharides, RG4 (Rha-GalA-Rha-GalA: RG4) and the RG hexasaccharide (Rha-GalA-Rha-GalA-Rha-GalA: RG6), prepared from potato rhamnogalacturonan I Penicillium chrysogenum L-rhamnose + ?
-
?
3.2.1.174 rhamnogalacturonan oligosaccharide + H2O two oligosaccharides, RG4 (Rha-GalA-Rha-GalA: RG4) and the RG hexasaccharide (Rha-GalA-Rha-GalA-Rha-GalA: RG6), prepared from potato rhamnogalacturonan I Penicillium chrysogenum 31B L-rhamnose + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.174 monomer 1 * 82000, glycosylated enzyme, SDS-PAGE, 1 * 67000, deglycosylated enzyme, SDS-PAGE, 1 * 101000, recombinant enzyme, SDS-PAGE Penicillium chrysogenum
3.2.1.174 More peptide mapping Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.174 alpha-L-rhamnosidase
-
Penicillium chrysogenum
3.2.1.174 PcRGRH78A
-
Penicillium chrysogenum
3.2.1.174 RG rhamnohydrolase
-
Penicillium chrysogenum
3.2.1.174 RGRH
-
Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.174 37
-
assay at Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.174 5
-
assay at Penicillium chrysogenum

General Information

EC Number General Information Comment Organism
3.2.1.174 evolution the enzyme belongs to the glycoside hydrolase family 78, GH78, group 3. Enzyme PcRGRH78A contains a bacterial alpha-L-rhamnosidase (PF05592) domain, but shows no activity with rhamnogalacturonan oligosaccharides Penicillium chrysogenum