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Literature summary extracted from

  • Reddy, T.S.; Sprecher, H.; Bazan, N.G.
    Long-chain acyl-coenzyme A synthetase from rat liver brain microsomes. Kinetic studies using [1-14C]docosahexaenoic acid substrate (1984), Eur. J. Biochem., 145, 21-29.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.2.1.3 Triton X-100 enhances activity Rattus norvegicus

General Stability

EC Number General Stability Organism
6.2.1.3 36% loss of activity after freezing and thawing Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.3 arachidonate
-
 Rattus norvegicus
6.2.1.3 docosahexaenoate unlabeled, inhibition of docosahexaenoate activation Rattus norvegicus
6.2.1.3 fatty acids saturated fatty acids do not inhibit activation of docosahexaenoate or palmitate. Unsaturated fatty acids, except nervonic acid, inhibit the activation of docosahexaenoate acid. Moderate inhibition by oleate, linoleate, eicosapentanoate, and palmitate Rattus norvegicus
6.2.1.3 Hg2+
-
 Rattus norvegicus
6.2.1.3 Pb2+
-
 Rattus norvegicus
6.2.1.3 SDS
-
 Rattus norvegicus
6.2.1.3 sodium deoxycholate
-
 Rattus norvegicus
6.2.1.3 Triton X-100 slight inhibition when incubated with microsomes at 0°C for 30 min Rattus norvegicus
6.2.1.3 Zwittergent 3-12 inhibition below the critical micellar concentration, 0.12%. below this concentrations no inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.3 0.00488
-
 CoA
-
 Rattus norvegicus
6.2.1.3 0.185
-
 ATP
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.3 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.3 Mg2+ required Rattus norvegicus
6.2.1.3 Mg2+ Km: 0.555 mM Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.3 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.3 brain
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.3 ATP + docosahexaenoate + CoA
-
 Rattus norvegicus AMP + diphosphate + docosahexaenoyl-CoA
-
 ?
6.2.1.3 ATP + palmitate + CoA
-
 Rattus norvegicus AMP + diphosphate + palmitoyl-CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.3 45
-
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.3 37
-
 half-life is 26.5 min Rattus norvegicus
6.2.1.3 45
-
 half-life: 3.3 min Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.3 8
-
-
 Rattus norvegicus