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Literature summary extracted from

  • Narayanan, K.; Parameswaran, B.; Pandey, A.
    Production of chitin deacetylase by Aspergillus flavus in submerged conditions (2016), Prep. Biochem. Biotechnol., 46, 501-508 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.41 synthesis chitosan produced by the enzymatic method offers the possibility of a controlled, non-degradable deacetylation process that results in a more regular pattern of deacetylation Aspergillus flavus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.41 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis Aspergillus flavus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.41 additional information total inhibition of CDA production when Ag2+, Co2+, Hg2+, Mn2+ and Ni2+ are added to the medium even at 5 mM concentration Aspergillus flavus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.41 extracellular
-
 Aspergillus flavus
-
-
3.5.1.41 additional information CDA in fungi are divided broadly into two subgroups: intracellular CDA and extracellular CDA. Intracellular CDA is secreted into the periplasm while extracellular chitin deacetylase is secreted into the external medium Aspergillus flavus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.41 Mg2+ activates Aspergillus flavus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.41 acetylated chitin polymer + H2O Aspergillus flavus
-
 ?
-
 ?
3.5.1.41 acetylated chitin polymer + H2O Aspergillus flavus P6B2
-
 ?
-
 ?
3.5.1.41 acetylated chitosan polymer + H2O Aspergillus flavus
-
 ?
-
 ?
3.5.1.41 acetylated chitosan polymer + H2O Aspergillus flavus P6B2
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Aspergillus flavus A0A3M7JTD4 isolated from environmental samples collected from coastal regions of South Kerala, India
-
3.5.1.41 Aspergillus flavus P6B2 A0A3M7JTD4 isolated from environmental samples collected from coastal regions of South Kerala, India
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.41 cell suspension culture optimization of CDA production method and parameters, detailed overview. Total inhibition of CDA production when Ag2+, Co2+, Hg2+, Mn2+ and Ni2+ are added to the medium even at 5 mM concentration Aspergillus flavus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 acetylated chitin polymer + H2O
-
 Aspergillus flavus ?
-
 ?
3.5.1.41 acetylated chitin polymer + H2O
-
 Aspergillus flavus P6B2 ?
-
 ?
3.5.1.41 acetylated chitosan polymer + H2O
-
 Aspergillus flavus ?
-
 ?
3.5.1.41 acetylated chitosan polymer + H2O
-
 Aspergillus flavus P6B2 ?
-
 ?
3.5.1.41 colloidal chitin + H2O
-
 Aspergillus flavus ?
-
 ?
3.5.1.41 colloidal chitin + H2O
-
 Aspergillus flavus P6B2 ?
-
 ?
3.5.1.41 additional information CDA catalyzes the deacetylation of monomeric acetyl glucosamine residues of chitin by multiple attack mechanism Aspergillus flavus ?
-
 ?
3.5.1.41 additional information CDA catalyzes the deacetylation of monomeric acetyl glucosamine residues of chitin by multiple attack mechanism Aspergillus flavus P6B2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.41 CDA
-
 Aspergillus flavus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.41 50
-
 assay at Aspergillus flavus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.41 6.5
-
 assay at Aspergillus flavus

Expression

EC Number Organism Comment Expression
3.5.1.41 Aspergillus flavus total inhibition of CDA production when Ag2+, Co2+, Hg2+, Mn2+ and Ni2+ are added to the medium even at 5 mM concentration, Cu2+ leads to decreased enzyme production in submerged culture down
3.5.1.41 Aspergillus flavus non-ionic surfactants like Tween 40, Tween 60 and Tween 80 increase CDA production, Ca2+ also leads to increased enzyme production in submerged culture up

General Information

EC Number General Information Comment Organism
3.5.1.41 physiological function the CDA enzyme plays a significant role only during the growth phase of the fungus Aspergillus flavus