EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.2.1 | expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
2.1.2.1 | expressed in Escherichia coli BL21(DE3) cells | Plasmodium vivax |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.1 | tetrahydrofolate | strong and pH-dependent substrate inhibition due to the formation of the enzyme-glycine:tetrahydrofolate dead-end complex (at low pH values) | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | 0.005 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 0.011 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 0.018 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 0.038 | - |
tetrahydrofolate | at pH 8.3, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.042 | - |
tetrahydrofolate | at pH 7.9, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.048 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.049 | - |
tetrahydrofolate | at pH 7.9, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 0.051 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.054 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.094 | - |
tetrahydrofolate | at pH 8.3, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 0.11 | - |
L-serine | at pH 7.9, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.12 | - |
L-serine | at pH 8.3, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.16 | - |
L-serine | at pH 8.3, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 0.18 | - |
L-serine | at pH 7.9, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 0.2 | - |
L-serine | at pH 7.5, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.22 | - |
L-serine | at pH 7.5, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 0.23 | - |
L-serine | at pH 7.1, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.28 | - |
L-serine | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 0.3 | - |
L-serine | at pH 6.6, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.42 | - |
L-serine | at pH 6.6, temperature not specified in the publication | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.1.2.1 | cytosol | - |
Homo sapiens | 5829 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.1 | 5,10-methylenetetrahydrofolate + glycine + H2O | Homo sapiens | - |
tetrahydrofolate + L-serine | - |
r | |
2.1.2.1 | 5,10-methylenetetrahydrofolate + glycine + H2O | Plasmodium vivax | - |
tetrahydrofolate + L-serine | - |
r | |
2.1.2.1 | tetrahydrofolate + L-serine | Homo sapiens | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
2.1.2.1 | tetrahydrofolate + L-serine | Plasmodium vivax | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.1 | Homo sapiens | - |
- |
- |
2.1.2.1 | Plasmodium vivax | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.1 | polyethyleneimine precipitation, Q-Sepharose column chromatography and Sephacyl S-200 gel filtration | Homo sapiens |
2.1.2.1 | polyethyleneimine precipitation, SP-Sepharose column chromatography and DEAE-Sepharose column chromatography | Plasmodium vivax |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.1 | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Homo sapiens | tetrahydrofolate + L-serine | - |
r | |
2.1.2.1 | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Plasmodium vivax | tetrahydrofolate + L-serine | - |
r | |
2.1.2.1 | tetrahydrofolate + L-serine | - |
Homo sapiens | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
2.1.2.1 | tetrahydrofolate + L-serine | - |
Plasmodium vivax | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.2.1 | serine hydroxymethyltransferase | - |
Homo sapiens |
2.1.2.1 | serine hydroxymethyltransferase | - |
Plasmodium vivax |
2.1.2.1 | SHMT | - |
Homo sapiens |
2.1.2.1 | SHMT | - |
Plasmodium vivax |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | 0.41 | - |
L-serine | at pH 8.3, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.41 | - |
tetrahydrofolate | at pH 8.3, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.57 | - |
L-serine | at pH 7.9, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.57 | - |
tetrahydrofolate | at pH 7.9, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.81 | - |
L-serine | at pH 7.5, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 0.81 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 1.1 | - |
L-serine | at pH 6.6, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 1.1 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 1.41 | - |
L-serine | at pH 7.1, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 1.41 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 19.6 | - |
L-serine | at pH 8.3, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 19.6 | - |
tetrahydrofolate | at pH 8.3, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 19.8 | - |
L-serine | at pH 7.5, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 19.8 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 19.8 | - |
L-serine | at pH 7.9, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 19.8 | - |
tetrahydrofolate | at pH 7.9, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 21 | - |
L-serine | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 21 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 30 | - |
L-serine | at pH 6.6, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 30 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 6.6 | - |
- |
Homo sapiens |
2.1.2.1 | 7 | - |
- |
Plasmodium vivax |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 6.6 | 8.4 | the catalytic efficiency of the enzyme for tetrahydrofolate is about 29fold greater at pH 6.6 than at pH 8.3 | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.1 | pyridoxal 5'-phosphate | - |
Homo sapiens | |
2.1.2.1 | pyridoxal 5'-phosphate | - |
Plasmodium vivax |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | 0.14 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 0.16 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 0.27 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | 3.4 | - |
L-serine | at pH 8.3, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 3.7 | - |
L-serine | at pH 6.6, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 4.1 | - |
L-serine | at pH 7.5, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 5.2 | - |
L-serine | at pH 7.9, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 6.1 | - |
L-serine | at pH 7.1, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 11 | - |
tetrahydrofolate | at pH 8.3, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 14 | - |
tetrahydrofolate | at pH 7.9, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 15 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 22 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 29 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Plasmodium vivax | |
2.1.2.1 | 71 | - |
L-serine | at pH 6.6, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 75 | - |
L-serine | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 90 | - |
L-serine | at pH 7.5, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 110 | - |
L-serine | at pH 7.9, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 123 | - |
L-serine | at pH 8.3, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 209 | - |
tetrahydrofolate | at pH 8.3, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 404 | - |
tetrahydrofolate | at pH 7.9, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 1100 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 1909 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
2.1.2.1 | 6000 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Homo sapiens |