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Literature summary extracted from

  • Amornwatcharapong, W.; Maenpuen, S.; Chitnumsub, P.; Leartsakulpanich, U.; Chaiyen, P.
    Human and Plasmodium serine hydroxymethyltransferases differ in rate-limiting steps and pH-dependent substrate inhibition behavior (2017), Arch. Biochem. Biophys., 630, 91-100 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expressed in Escherichia coli BL21(DE3) cells Homo sapiens
2.1.2.1 expressed in Escherichia coli BL21(DE3) cells Plasmodium vivax

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 tetrahydrofolate strong and pH-dependent substrate inhibition due to the formation of the enzyme-glycine:tetrahydrofolate dead-end complex (at low pH values) Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.005
-
tetrahydrofolate at pH 6.6, temperature not specified in the publication Homo sapiens
2.1.2.1 0.011
-
tetrahydrofolate at pH 7.1, temperature not specified in the publication Homo sapiens
2.1.2.1 0.018
-
tetrahydrofolate at pH 7.5, temperature not specified in the publication Homo sapiens
2.1.2.1 0.038
-
tetrahydrofolate at pH 8.3, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.042
-
tetrahydrofolate at pH 7.9, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.048
-
tetrahydrofolate at pH 7.1, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.049
-
tetrahydrofolate at pH 7.9, temperature not specified in the publication Homo sapiens
2.1.2.1 0.051
-
tetrahydrofolate at pH 6.6, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.054
-
tetrahydrofolate at pH 7.5, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.094
-
tetrahydrofolate at pH 8.3, temperature not specified in the publication Homo sapiens
2.1.2.1 0.11
-
L-serine at pH 7.9, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.12
-
L-serine at pH 8.3, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.16
-
L-serine at pH 8.3, temperature not specified in the publication Homo sapiens
2.1.2.1 0.18
-
L-serine at pH 7.9, temperature not specified in the publication Homo sapiens
2.1.2.1 0.2
-
L-serine at pH 7.5, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.22
-
L-serine at pH 7.5, temperature not specified in the publication Homo sapiens
2.1.2.1 0.23
-
L-serine at pH 7.1, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.28
-
L-serine at pH 7.1, temperature not specified in the publication Homo sapiens
2.1.2.1 0.3
-
L-serine at pH 6.6, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.42
-
L-serine at pH 6.6, temperature not specified in the publication Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytosol
-
Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Homo sapiens
-
tetrahydrofolate + L-serine
-
r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Plasmodium vivax
-
tetrahydrofolate + L-serine
-
r
2.1.2.1 tetrahydrofolate + L-serine Homo sapiens
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r
2.1.2.1 tetrahydrofolate + L-serine Plasmodium vivax
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Homo sapiens
-
-
-
2.1.2.1 Plasmodium vivax
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 polyethyleneimine precipitation, Q-Sepharose column chromatography and Sephacyl S-200 gel filtration Homo sapiens
2.1.2.1 polyethyleneimine precipitation, SP-Sepharose column chromatography and DEAE-Sepharose column chromatography Plasmodium vivax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
Homo sapiens tetrahydrofolate + L-serine
-
r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
Plasmodium vivax tetrahydrofolate + L-serine
-
r
2.1.2.1 tetrahydrofolate + L-serine
-
Homo sapiens 5,10-methylenetetrahydrofolate + glycine + H2O
-
r
2.1.2.1 tetrahydrofolate + L-serine
-
Plasmodium vivax 5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
Homo sapiens
2.1.2.1 serine hydroxymethyltransferase
-
Plasmodium vivax
2.1.2.1 SHMT
-
Homo sapiens
2.1.2.1 SHMT
-
Plasmodium vivax

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 0.41
-
L-serine at pH 8.3, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.41
-
tetrahydrofolate at pH 8.3, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.57
-
L-serine at pH 7.9, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.57
-
tetrahydrofolate at pH 7.9, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.81
-
L-serine at pH 7.5, temperature not specified in the publication Plasmodium vivax
2.1.2.1 0.81
-
tetrahydrofolate at pH 7.5, temperature not specified in the publication Plasmodium vivax
2.1.2.1 1.1
-
L-serine at pH 6.6, temperature not specified in the publication Plasmodium vivax
2.1.2.1 1.1
-
tetrahydrofolate at pH 6.6, temperature not specified in the publication Plasmodium vivax
2.1.2.1 1.41
-
L-serine at pH 7.1, temperature not specified in the publication Plasmodium vivax
2.1.2.1 1.41
-
tetrahydrofolate at pH 7.1, temperature not specified in the publication Plasmodium vivax
2.1.2.1 19.6
-
L-serine at pH 8.3, temperature not specified in the publication Homo sapiens
2.1.2.1 19.6
-
tetrahydrofolate at pH 8.3, temperature not specified in the publication Homo sapiens
2.1.2.1 19.8
-
L-serine at pH 7.5, temperature not specified in the publication Homo sapiens
2.1.2.1 19.8
-
tetrahydrofolate at pH 7.5, temperature not specified in the publication Homo sapiens
2.1.2.1 19.8
-
L-serine at pH 7.9, temperature not specified in the publication Homo sapiens
2.1.2.1 19.8
-
tetrahydrofolate at pH 7.9, temperature not specified in the publication Homo sapiens
2.1.2.1 21
-
L-serine at pH 7.1, temperature not specified in the publication Homo sapiens
2.1.2.1 21
-
tetrahydrofolate at pH 7.1, temperature not specified in the publication Homo sapiens
2.1.2.1 30
-
L-serine at pH 6.6, temperature not specified in the publication Homo sapiens
2.1.2.1 30
-
tetrahydrofolate at pH 6.6, temperature not specified in the publication Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 6.6
-
-
Homo sapiens
2.1.2.1 7
-
-
Plasmodium vivax

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.2.1 6.6 8.4 the catalytic efficiency of the enzyme for tetrahydrofolate is about 29fold greater at pH 6.6 than at pH 8.3 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
Homo sapiens
2.1.2.1 pyridoxal 5'-phosphate
-
Plasmodium vivax

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.2.1 0.14
-
tetrahydrofolate at pH 6.6, temperature not specified in the publication Homo sapiens
2.1.2.1 0.16
-
tetrahydrofolate at pH 7.1, temperature not specified in the publication Homo sapiens
2.1.2.1 0.27
-
tetrahydrofolate at pH 7.5, temperature not specified in the publication Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.2.1 3.4
-
L-serine at pH 8.3, temperature not specified in the publication Plasmodium vivax
2.1.2.1 3.7
-
L-serine at pH 6.6, temperature not specified in the publication Plasmodium vivax
2.1.2.1 4.1
-
L-serine at pH 7.5, temperature not specified in the publication Plasmodium vivax
2.1.2.1 5.2
-
L-serine at pH 7.9, temperature not specified in the publication Plasmodium vivax
2.1.2.1 6.1
-
L-serine at pH 7.1, temperature not specified in the publication Plasmodium vivax
2.1.2.1 11
-
tetrahydrofolate at pH 8.3, temperature not specified in the publication Plasmodium vivax
2.1.2.1 14
-
tetrahydrofolate at pH 7.9, temperature not specified in the publication Plasmodium vivax
2.1.2.1 15
-
tetrahydrofolate at pH 7.5, temperature not specified in the publication Plasmodium vivax
2.1.2.1 22
-
tetrahydrofolate at pH 6.6, temperature not specified in the publication Plasmodium vivax
2.1.2.1 29
-
tetrahydrofolate at pH 7.1, temperature not specified in the publication Plasmodium vivax
2.1.2.1 71
-
L-serine at pH 6.6, temperature not specified in the publication Homo sapiens
2.1.2.1 75
-
L-serine at pH 7.1, temperature not specified in the publication Homo sapiens
2.1.2.1 90
-
L-serine at pH 7.5, temperature not specified in the publication Homo sapiens
2.1.2.1 110
-
L-serine at pH 7.9, temperature not specified in the publication Homo sapiens
2.1.2.1 123
-
L-serine at pH 8.3, temperature not specified in the publication Homo sapiens
2.1.2.1 209
-
tetrahydrofolate at pH 8.3, temperature not specified in the publication Homo sapiens
2.1.2.1 404
-
tetrahydrofolate at pH 7.9, temperature not specified in the publication Homo sapiens
2.1.2.1 1100
-
tetrahydrofolate at pH 7.5, temperature not specified in the publication Homo sapiens
2.1.2.1 1909
-
tetrahydrofolate at pH 7.1, temperature not specified in the publication Homo sapiens
2.1.2.1 6000
-
tetrahydrofolate at pH 6.6, temperature not specified in the publication Homo sapiens