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Literature summary extracted from

  • Zhang, M.; Wu, W.; Chen, Z.
    Structure and function of cytoplasmic serine hydroxymethyltransferase from Pichia pastoris (2018), Biochem. Biophys. Res. Commun., 496, 753-757 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expressed in Escherichia coli BL21(DE3) cells Komagataella pastoris

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.1 hanging drop vapor diffusion method, using 18% (w/v) PEG 8000, 0.1 M MES pH 6.0, 0.2 M Ca(OAc)2 Komagataella pastoris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytoplasm
-
 Komagataella pastoris 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Komagataella pastoris
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Komagataella pastoris X-33
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine Komagataella pastoris
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
2.1.2.1 tetrahydrofolate + L-serine Komagataella pastoris X-33
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Komagataella pastoris F2QZA4
-
-
2.1.2.1 Komagataella pastoris X-33 F2QZA4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 Ni2+-chelating column chromatography, SP Sepharose column chromatography and Superdex S200 gel filtration Komagataella pastoris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Komagataella pastoris tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Komagataella pastoris X-33 tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine
-
 Komagataella pastoris 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
2.1.2.1 tetrahydrofolate + L-serine
-
 Komagataella pastoris X-33 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.1 ? x * 50000, SDS-PAGE Komagataella pastoris

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Komagataella pastoris
2.1.2.1 SHMT
-
 Komagataella pastoris

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Komagataella pastoris