EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.1 | EDTA | 101.25% activity at 1% (v/v) | Glutamicibacter nicotianae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.2.1 | expressed in Escherichia coli DE3 cells | Glutamicibacter nicotianae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.2.1 | I249L | the catalytic efficiency of the mutant is 2.78fold higher than that of the wild type enzyme | Glutamicibacter nicotianae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.1 | CO(NH2)2 | 60.59% residual activity at 1 mM | Glutamicibacter nicotianae | |
2.1.2.1 | Co2+ | 60.03% residual activity at 1 mM | Glutamicibacter nicotianae | |
2.1.2.1 | Cu2+ | 8.95% residual activity at 1 mM | Glutamicibacter nicotianae | |
2.1.2.1 | dithiothreitol | 59.29% residual activity at 1 mM | Glutamicibacter nicotianae | |
2.1.2.1 | Fe2+ | complete inhibition at 1 mM | Glutamicibacter nicotianae | |
2.1.2.1 | Hg2+ | complete inhibition at 1 mM | Glutamicibacter nicotianae | |
2.1.2.1 | Mn2+ | 60.81% residual activity at 1 mM | Glutamicibacter nicotianae | |
2.1.2.1 | additional information | the enzyme is not significantly influenced in activity by NH4+, Sl2+, Ca2+, Pb2+, SDS and CTAB | Glutamicibacter nicotianae | |
2.1.2.1 | Zn2+ | 2.91% residual activity at 1 mM | Glutamicibacter nicotianae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | 57.86 | - |
DL-3-phenylserine | wild type enzyme, at pH 7.8 and 30°C | Glutamicibacter nicotianae | |
2.1.2.1 | 61.95 | - |
DL-3-phenylserine | mutant enzyme I249L, at pH 7.8 and 30°C | Glutamicibacter nicotianae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.1 | Ba2+ | 102.58% activity at 1 mM | Glutamicibacter nicotianae | |
2.1.2.1 | Mg2+ | 108.38% activity at 1 mM | Glutamicibacter nicotianae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.1 | 5,10-methylenetetrahydrofolate + glycine + H2O | Glutamicibacter nicotianae | - |
tetrahydrofolate + L-serine | - |
r | |
2.1.2.1 | tetrahydrofolate + L-serine | Glutamicibacter nicotianae | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.1 | Glutamicibacter nicotianae | U5TRK6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.1 | glutathione Sepharose column chromatography | Glutamicibacter nicotianae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.1 | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Glutamicibacter nicotianae | tetrahydrofolate + L-serine | - |
r | |
2.1.2.1 | DL-3-phenylserine + ? | - |
Glutamicibacter nicotianae | benzaldehyde + ? | - |
? | |
2.1.2.1 | tetrahydrofolate + L-serine | - |
Glutamicibacter nicotianae | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.2.1 | ? | x * 47300, calculated from amino acid sequence | Glutamicibacter nicotianae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.2.1 | GlyA | - |
Glutamicibacter nicotianae |
2.1.2.1 | serine hydroxymethyltransferase | - |
Glutamicibacter nicotianae |
2.1.2.1 | SHMT | - |
Glutamicibacter nicotianae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 40 | - |
- |
Glutamicibacter nicotianae |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 30 | 65 | the enzyme retains over 50% of the maximal activity at 30-65°C | Glutamicibacter nicotianae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 35 | 55 | after 1 h incubation under pH 7.5, the enzyme retains over 35% of its maximal activity from 35°C to 45°C, but less than 15% at 55°C | Glutamicibacter nicotianae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | 1.6 | - |
DL-3-phenylserine | wild type enzyme, at pH 7.8 and 30°C | Glutamicibacter nicotianae | |
2.1.2.1 | 4.8 | - |
DL-3-phenylserine | mutant enzyme I249L, at pH 7.8 and 30°C | Glutamicibacter nicotianae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 7.5 | - |
- |
Glutamicibacter nicotianae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 7 | 8 | the enzyme shows more than 40% activity between pH 7.0 and 8.0. The enzyme displays less than 20% of its maximal activity at pH 5.5 and nearly no activity is detected below pH 2.5 | Glutamicibacter nicotianae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 5.5 | 8.5 | the enzyme retains over 80% of the maximum activity over a pH range from 7.0 to 8.5 for 24 h at 4°C, and more than 70% of the maximal activity at pH 6.5. However, the enzyme exhibits a rapid decrease in activity at pH 5.5 | Glutamicibacter nicotianae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.1 | pyridoxal 5'-phosphate | - |
Glutamicibacter nicotianae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | 0.028 | - |
DL-3-phenylserine | wild type enzyme, at pH 7.8 and 30°C | Glutamicibacter nicotianae | |
2.1.2.1 | 0.077 | - |
DL-3-phenylserine | mutant enzyme I249L, at pH 7.8 and 30°C | Glutamicibacter nicotianae |