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Literature summary extracted from

  • Rolfsmeier, M.; Haseltine, C.
    The RadA recombinase and paralogs of the hyperthermophilic archaeon Sulfolobus solfataricus (2018), Methods Enzymol., 600, 255-284 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.4.B7 gene radA, recombinant overexpression in Escherichia coli strain Rosetta Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.4.B7 Mg2+ required Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.4.B7 ATP + H2O Saccharolobus solfataricus
-
 ADP + phosphate
-
 ?
3.6.4.B7 ATP + H2O Saccharolobus solfataricus P2
-
 ADP + phosphate
-
 ?
3.6.4.B7 ATP + H2O Saccharolobus solfataricus JCM 11322
-
 ADP + phosphate
-
 ?
3.6.4.B7 ATP + H2O Saccharolobus solfataricus ATCC 35092
-
 ADP + phosphate
-
 ?
3.6.4.B7 ATP + H2O Saccharolobus solfataricus DSM 1617
-
 ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.B7 Saccharolobus solfataricus Q55075 i.e. Sulfolobus solfataricus
-
3.6.4.B7 Saccharolobus solfataricus ATCC 35092 Q55075 i.e. Sulfolobus solfataricus
-
3.6.4.B7 Saccharolobus solfataricus DSM 1617 Q55075 i.e. Sulfolobus solfataricus
-
3.6.4.B7 Saccharolobus solfataricus JCM 11322 Q55075 i.e. Sulfolobus solfataricus
-
3.6.4.B7 Saccharolobus solfataricus P2 Q55075 i.e. Sulfolobus solfataricus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.4.B7 recombinant enzyme from Escherichia coli strain Rosetta by affinity and anion exchange chromatography, and dialysis, protein concentration using PEG, method, overview Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.B7 ATP + H2O
-
 Saccharolobus solfataricus ADP + phosphate
-
 ?
3.6.4.B7 ATP + H2O
-
 Saccharolobus solfataricus P2 ADP + phosphate
-
 ?
3.6.4.B7 ATP + H2O
-
 Saccharolobus solfataricus JCM 11322 ADP + phosphate
-
 ?
3.6.4.B7 ATP + H2O
-
 Saccharolobus solfataricus ATCC 35092 ADP + phosphate
-
 ?
3.6.4.B7 ATP + H2O
-
 Saccharolobus solfataricus DSM 1617 ADP + phosphate
-
 ?
3.6.4.B7 additional information methods for DNA and ATP binding assays, strand invasion and exchange, and ATPase assays, detailed overview Saccharolobus solfataricus ?
-
-
3.6.4.B7 additional information methods for DNA and ATP binding assays, strand invasion and exchange, and ATPase assays, detailed overview Saccharolobus solfataricus P2 ?
-
-
3.6.4.B7 additional information methods for DNA and ATP binding assays, strand invasion and exchange, and ATPase assays, detailed overview Saccharolobus solfataricus JCM 11322 ?
-
-
3.6.4.B7 additional information methods for DNA and ATP binding assays, strand invasion and exchange, and ATPase assays, detailed overview Saccharolobus solfataricus ATCC 35092 ?
-
-
3.6.4.B7 additional information methods for DNA and ATP binding assays, strand invasion and exchange, and ATPase assays, detailed overview Saccharolobus solfataricus DSM 1617 ?
-
-

Subunits

EC Number Subunits Comment Organism
3.6.4.B7 ? x * 35900, about, sequence calculation Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.6.4.B7 RadA
-
 Saccharolobus solfataricus
3.6.4.B7 RadA recombinase
-
 Saccharolobus solfataricus
3.6.4.B7 SsoRadA
-
 Saccharolobus solfataricus
3.6.4.B7 SsoRadA recombinase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.4.B7 70 80
-
 Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.4.B7 7.5
-
 assay at Saccharolobus solfataricus