Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Djavaheri, M.; Ma, L.; Klessig, D.F.; Mithoefer, A.; Gropp, G.; Borhan, H.
    Mimicking the host regulation of salicylic acid a virulence strategy by the clubroot pathogen Plasmodiophora brassicae (2019), Mol. Plant Microbe Interact., 32, 296-305 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.273 gene BSMT, recombinant expression of 35S::PbBSMT in planta in Arabidopsis thaliana reduces salicylate (SA) levels and increases susceptibility to clubroot, Plasmodiophora brassicae Plasmodiophora brassicae
2.1.1.274 gene BSMT, recombinant expression of 35S::PbBSMT in planta in Arabidopsis thaliana reduces salicylate (SA) levels and increases susceptibility to clubroot, Plasmodiophora brassicae Plasmodiophora brassicae

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.273 additional information an AtBSMT1 T-DNA knockout (AtBSMT1 KO, SALK_140496) is transformed by AtBSMT1 (Ask6) and PbBSMT (PsK5) and their susceptibility to clubroot is assessed. The infection rate is similar in the AtBSMT1 KO and AsK6 and comparable to that in wild-type. In contrast, PsK5 plants produce significantly larger galls than produced by the AtBSMT1 knockout Arabidopsis thaliana
2.1.1.273 additional information an AtBSMT1 T-DNA knockout (AtBSMT1 KO, SALK_140496) is transformed by AtBSMT1 (Ask6) and PbBSMT (PsK5) and their susceptibility to clubroot is assessed. The infection rate is similar in the AtBSMT1 KO and AsK6 and comparable to that in wild-type. In contrast, PsK5 plants produce significantly larger galls than produced by the AtBSMT1 knockout Plasmodiophora brassicae
2.1.1.274 additional information an AtBSMT1 T-DNA knockout (AtBSMT1 KO, SALK_140496) is transformed by AtBSMT1 (Ask6) and PbBSMT (PsK5) and their susceptibility to clubroot is assessed. The infection rate is similar in the AtBSMT1 KO and AsK6 and comparable to that in wild-type. In contrast, PsK5 plants produce significantly larger galls than produced by the AtBSMT1 knockout Arabidopsis thaliana
2.1.1.274 additional information an AtBSMT1 T-DNA knockout (AtBSMT1 KO, SALK_140496) is transformed by AtBSMT1 (Ask6) and PbBSMT (PsK5) and their susceptibility to clubroot is assessed. The infection rate is similar in the AtBSMT1 KO and AsK6 and comparable to that in wild-type. In contrast, PsK5 plants produce significantly larger galls than produced by the AtBSMT1 knockout Plasmodiophora brassicae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.273 chloroplast
-
 Arabidopsis thaliana 9507
-
2.1.1.273 extracellular the enzyme is secreted Plasmodiophora brassicae
-
-
2.1.1.274 chloroplast
-
 Arabidopsis thaliana 9507
-
2.1.1.274 extracellular the enzyme is secreted Plasmodiophora brassicae
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.273 S-adenosyl-L-methionine + benzoate Arabidopsis thaliana
-
 methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.273 S-adenosyl-L-methionine + benzoate Plasmodiophora brassicae
-
 methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.273 S-adenosyl-L-methionine + benzoate Arabidopsis thaliana Col-0
-
 methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.273 S-adenosyl-L-methionine + salicylate Arabidopsis thaliana
-
 methyl salicylate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.273 S-adenosyl-L-methionine + salicylate Plasmodiophora brassicae
-
 methyl salicylate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.273 S-adenosyl-L-methionine + salicylate Arabidopsis thaliana Col-0
-
 methyl salicylate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + benzoate Arabidopsis thaliana
-
 methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + benzoate Plasmodiophora brassicae
-
 methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + benzoate Arabidopsis thaliana Col-0
-
 methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylate Arabidopsis thaliana
-
 methyl salicylate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylate Plasmodiophora brassicae
-
 methyl salicylate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylate Arabidopsis thaliana Col-0
-
 methyl salicylate + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.273 Arabidopsis thaliana Q6XMI3
-
-
2.1.1.273 Arabidopsis thaliana Col-0 Q6XMI3
-
-
2.1.1.273 Plasmodiophora brassicae R4I7S9
-
-
2.1.1.274 Arabidopsis thaliana Q6XMI3
-
-
2.1.1.274 Arabidopsis thaliana Col-0 Q6XMI3
-
-
2.1.1.274 Plasmodiophora brassicae R4I7S9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.273 leaf
-
 Arabidopsis thaliana
-
2.1.1.274 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.273 S-adenosyl-L-methionine + benzoate
-
 Arabidopsis thaliana methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.273 S-adenosyl-L-methionine + benzoate
-
 Plasmodiophora brassicae methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.273 S-adenosyl-L-methionine + benzoate
-
 Arabidopsis thaliana Col-0 methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.273 S-adenosyl-L-methionine + salicylate
-
 Arabidopsis thaliana methyl salicylate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.273 S-adenosyl-L-methionine + salicylate
-
 Plasmodiophora brassicae methyl salicylate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.273 S-adenosyl-L-methionine + salicylate
-
 Arabidopsis thaliana Col-0 methyl salicylate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + benzoate
-
 Arabidopsis thaliana methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + benzoate
-
 Plasmodiophora brassicae methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + benzoate
-
 Arabidopsis thaliana Col-0 methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylate
-
 Arabidopsis thaliana methyl salicylate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylate
-
 Plasmodiophora brassicae methyl salicylate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylate
-
 Arabidopsis thaliana Col-0 methyl salicylate + S-adenosyl-L-homocysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.273 More PbBSMT has a salicylate- and an indole-3-acetic acid-binding domain Plasmodiophora brassicae
2.1.1.274 More PbBSMT has a salicylate- and an indole-3-acetic acid-binding domain Plasmodiophora brassicae

Synonyms

EC Number Synonyms Comment Organism
2.1.1.273 BSMT
-
 Arabidopsis thaliana
2.1.1.273 BSMT
-
 Plasmodiophora brassicae
2.1.1.273 More see also EC 2.1.1.274 Arabidopsis thaliana
2.1.1.273 More see also EC 2.1.1.274 Plasmodiophora brassicae
2.1.1.274 BSMT
-
 Arabidopsis thaliana
2.1.1.274 BSMT
-
 Plasmodiophora brassicae
2.1.1.274 More see also EC 2.1.1.273 Arabidopsis thaliana
2.1.1.274 More see also EC 2.1.1.273 Plasmodiophora brassicae

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.273 S-adenosyl-L-methionine
-
 Arabidopsis thaliana
2.1.1.273 S-adenosyl-L-methionine
-
 Plasmodiophora brassicae
2.1.1.274 S-adenosyl-L-methionine
-
 Arabidopsis thaliana
2.1.1.274 S-adenosyl-L-methionine
-
 Plasmodiophora brassicae

General Information

EC Number General Information Comment Organism
2.1.1.273 malfunction AtBSMT1-overexpressing plants are not more susceptible than wild-type to either Plasmodiophora brassicae or Albugo candida. Transgenic Arabidopsis thaliana and Nicotiana tabacum plants overexpressing PbBSMT exhibit increased susceptibility to virulent Pseudomonas syringae pv. tomato DC3000 and virulent Pseudomonas syringae pv. tabaci, respectively. Gene-mediated resistance to DC3000/AvrRpt2 and tobacco mosaic virus (TMV) is also compromised in Arabidopsis thaliana and Nicotiana tabacum cv. Xanthi-nc plants overexpressing PbBSMT, respectively. Transient expression of PbBSMT or AtBSMT1 in lower leaves of Nicotiana tabacum Xanthi-nc results in systemic acquired resistance (SAR)-like enhanced resistance to TMV in the distal systemic leaves. The development of a PbBSMT-mediated SAR-like phenotype is also dependent on the MeSA esterase activity of NtSABP2 in the systemic leaves. Phenotypes, overview Arabidopsis thaliana
2.1.1.273 malfunction basal salicylic acid (SA) levels in Arabidopsis thaliana plants that constitutively overexpress PbBSMT compared with those in Arabidopsis wild-type Col-0 are reduced approximately 80% versus only a 50% reduction in plants overexpressing AtBSMT1. PbBSMT-overexpressing plants are more susceptible to Plasmodiophora brassicae than wild-type plants, they also are partially compromised in nonhost resistance to Albugo candida. In contrast, AtBSMT1-overexpressing plants are not more susceptible than wild-type to either Plasmodiophora brassicae or Albugo candida. Furthermore, transgenic Arabidopsis thaliana and Nicotiana tabacum plants overexpressing PbBSMT exhibit increased susceptibility to virulent Pseudomonas syringae pv. tomato DC3000 and virulent Pseudomonas syringae pv. tabaci, respectively. Gene-mediated resistance to DC3000/AvrRpt2 and tobacco mosaic virus (TMV) is also compromised in Arabidopsis thaliana and Nicotiana tabacum cv. Xanthi-nc plants overexpressing PbBSMT, respectively. Transient expression of PbBSMT or AtBSMT1 in lower leaves of Nicotiana tabacum cv. Xanthi-nc results in systemic acquired resistance (SAR)-like enhanced resistance to TMV in the distal systemic leaves. The development of a PbBSMT-mediated SAR-like phenotype is also dependent on the MeSA esterase activity of NtSABP2 in the systemic leaves. Phenotypes, overview Plasmodiophora brassicae
2.1.1.273 physiological function mimicking the host regulation of salicylic acid: a virulence strategy by the clubroot pathogen Plasmodiophora brassicae, overview. The plant hormone salicylic acid (SA) plays a critical role in defense against biotrophic pathogens, e.g. Plasmodiophora brassicae, which is an obligate pathogen of crucifer species and the causal agent of clubroot disease of canola (Brassica napus), encoding a protein with very limited homology to benzoic acid (BA)/SA-methyltransferase, designated PbBSMT. Enzyme PbBSMT is an effector, which is secreted by Plasmodiophora brassicae into its host plant to deplete pathogen-induced SA accumulation. Plasmodiophora brassicae uses PbBSMTto overcome SA-mediated defenses by converting SA into inactive methyl salicylate (MeSA). PbBSMT suppresses local defense and provide evidence that PbBSMT is much more effective than endogenous Arabidopsis thaliana host enzyme AtBSMT1 at suppressing the levels of SA and its associated effects. PbBSMT is much more effective than AtBSMT1 at both reducing endogenous and exogenous SA levels and at suppressing multiple levels of resistance, including nonhost and basal resistance as well as pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) Plasmodiophora brassicae
2.1.1.273 physiological function mimicking the host regulation of salicylic acid: a virulence strategy by the clubroot pathogen Plasmodiophora brassicae, overview. The plant hormone salicylic acid (SA) plays a critical role in defense against biotrophic pathogens, e.g. Plasmodiophora brassicae, which is an obligate pathogen of crucifer species and the causal agent of clubroot disease of canola (Brassica napus). A pathogen salicylate methyltransferase, PbBSMT, suppresses local defense and provide evidence that PbBSMT is much more effective than endogenous Arabidopsis thaliana host methyltransferase enzyme AtBSMT1 at suppressing the levels of SA and its associated effects. PbBSMT is much more effective than AtBSMT1 at both reducing endogenous and exogenous SA levels and at suppressing multiple levels of resistance, including nonhost and basal resistance as well as pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) Arabidopsis thaliana
2.1.1.274 malfunction AtBSMT1-overexpressing plants are not more susceptible than wild-type to either Plasmodiophora brassicae or Albugo candida. Transgenic Arabidopsis thaliana and Nicotiana tabacum plants overexpressing PbBSMT exhibit increased susceptibility to virulent Pseudomonas syringae pv. tomato DC3000 and virulent Pseudomonas syringae pv. tabaci, respectively. Gene-mediated resistance to DC3000/AvrRpt2 and tobacco mosaic virus (TMV) is also compromised in Arabidopsis thaliana and Nicotiana tabacum cv. Xanthi-nc plants overexpressing PbBSMT, respectively. Transient expression of PbBSMT or AtBSMT1 in lower leaves of Nicotiana tabacum Xanthi-nc results in systemic acquired resistance (SAR)-like enhanced resistance to TMV in the distal systemic leaves. The development of a PbBSMT-mediated SAR-like phenotype is also dependent on the MeSA esterase activity of NtSABP2 in the systemic leaves. Phenotypes, overview Arabidopsis thaliana
2.1.1.274 malfunction basal salicylic acid (SA) levels in Arabidopsis thaliana plants that constitutively overexpress PbBSMT compared with those in Arabidopsis wild-type Col-0 are reduced approximately 80% versus only a 50% reduction in plants overexpressing AtBSMT1. PbBSMT-overexpressing plants are more susceptible to Plasmodiophora brassicae than wild-type plants, they also are partially compromised in nonhost resistance to Albugo candida. In contrast, AtBSMT1-overexpressing plants are not more susceptible than wild-type to either Plasmodiophora brassicae or Albugo candida. Furthermore, transgenic Arabidopsis thaliana and Nicotiana tabacum plants overexpressing PbBSMT exhibit increased susceptibility to virulent Pseudomonas syringae pv. tomato DC3000 and virulent Pseudomonas syringae pv. tabaci, respectively. Gene-mediated resistance to DC3000/AvrRpt2 and tobacco mosaic virus (TMV) is also compromised in Arabidopsis thaliana and Nicotiana tabacum cv. Xanthi-nc plants overexpressing PbBSMT, respectively. Transient expression of PbBSMT or AtBSMT1 in lower leaves of Nicotiana tabacum cv. Xanthi-nc results in systemic acquired resistance (SAR)-like enhanced resistance to TMV in the distal systemic leaves. The development of a PbBSMT-mediated SAR-like phenotype is also dependent on the MeSA esterase activity of NtSABP2 in the systemic leaves. Phenotypes, overview Plasmodiophora brassicae
2.1.1.274 physiological function mimicking the host regulation of salicylic acid: a virulence strategy by the clubroot pathogen Plasmodiophora brassicae, overview. The plant hormone salicylic acid (SA) plays a critical role in defense against biotrophic pathogens, e.g. Plasmodiophora brassicae, which is an obligate pathogen of crucifer species and the causal agent of clubroot disease of canola (Brassica napus), encoding a protein with very limited homology to benzoic acid (BA)/SA-methyltransferase, designated PbBSMT. Enzyme PbBSMT is an effector, which is secreted by Plasmodiophora brassicae into its host plant to deplete pathogen-induced SA accumulation. Plasmodiophora brassicae uses PbBSMTto overcome SA-mediated defenses by converting SA into inactive methyl salicylate (MeSA). PbBSMT suppresses local defense and provide evidence that PbBSMT is much more effective than endogenous Arabidopsis thaliana host enzyme AtBSMT1 at suppressing the levels of SA and its associated effects. PbBSMT is much more effective than AtBSMT1 at both reducing endogenous and exogenous SA levels and at suppressing multiple levels of resistance, including nonhost and basal resistance as well as pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) Plasmodiophora brassicae
2.1.1.274 physiological function mimicking the host regulation of salicylic acid: a virulence strategy by the clubroot pathogen Plasmodiophora brassicae, overview. The plant hormone salicylic acid (SA) plays a critical role in defense against biotrophic pathogens, e.g. Plasmodiophora brassicae, which is an obligate pathogen of crucifer species and the causal agent of clubroot disease of canola (Brassica napus). A pathogen salicylate methyltransferase, PbBSMT, suppresses local defense and provide evidence that PbBSMT is much more effective than endogenous Arabidopsis thaliana host methyltransferase enzyme AtBSMT1 at suppressing the levels of SA and its associated effects. PbBSMT is much more effective than AtBSMT1 at both reducing endogenous and exogenous SA levels and at suppressing multiple levels of resistance, including nonhost and basal resistance as well as pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) Arabidopsis thaliana