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Literature summary extracted from

  • McCarthy, R.; Mazon-Moya, M.; Moscoso, J.; Hao, Y.; Lam, J.; Bordi, C.; Mostowy, S.; Filloux, A.
    Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion (2017), Nat. Microbiol., 2, 17027 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.294 cyclic-di-GMP WarA binds to cyclic-di-GMP, which potentiates its methyltransferase activity Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.294 gene warA, quantitative RT-PCR expression analysis, recombinant expression of N-terminally His- or GFP-tagged enzyme, recombinat expression of GST-His-tagged wild-type and mutant enzymes in Escherichia coli strain B834(DE3) Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.294 additional information generation of a gene replacement mutants, deletion of the PA4379 (warA) gene in the Pseudomonas aeruginosa PAK chromosome Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.294 additional information Pseudomonas aeruginosa SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay ?
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-
2.1.1.294 additional information Pseudomonas aeruginosa ATCC 15692 SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay ?
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-
2.1.1.294 additional information Pseudomonas aeruginosa 1C SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay ?
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-
2.1.1.294 additional information Pseudomonas aeruginosa PRS 101 SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay ?
-
-
2.1.1.294 additional information Pseudomonas aeruginosa DSM 22644 SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay ?
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-
2.1.1.294 additional information Pseudomonas aeruginosa CIP 104116 SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay ?
-
-
2.1.1.294 additional information Pseudomonas aeruginosa LMG 12228 SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay ?
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-
2.1.1.294 additional information Pseudomonas aeruginosa JCM 14847 SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay ?
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-

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.294 Pseudomonas aeruginosa Q9HW23
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-
2.1.1.294 Pseudomonas aeruginosa 1C Q9HW23
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2.1.1.294 Pseudomonas aeruginosa ATCC 15692 Q9HW23
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2.1.1.294 Pseudomonas aeruginosa CIP 104116 Q9HW23
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-
2.1.1.294 Pseudomonas aeruginosa DSM 22644 Q9HW23
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-
2.1.1.294 Pseudomonas aeruginosa JCM 14847 Q9HW23
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-
2.1.1.294 Pseudomonas aeruginosa LMG 12228 Q9HW23
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-
2.1.1.294 Pseudomonas aeruginosa PRS 101 Q9HW23
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.294 recombinat GST-His-tagged wild-type and mutant enzymes from Escherichia coli strain B834(DE3) by nickel affinity chromatography and gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.294 additional information SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay Pseudomonas aeruginosa ?
-
-
2.1.1.294 additional information SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay Pseudomonas aeruginosa ATCC 15692 ?
-
-
2.1.1.294 additional information SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay Pseudomonas aeruginosa 1C ?
-
-
2.1.1.294 additional information SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay Pseudomonas aeruginosa PRS 101 ?
-
-
2.1.1.294 additional information SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay Pseudomonas aeruginosa DSM 22644 ?
-
-
2.1.1.294 additional information SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay Pseudomonas aeruginosa CIP 104116 ?
-
-
2.1.1.294 additional information SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay Pseudomonas aeruginosa LMG 12228 ?
-
-
2.1.1.294 additional information SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay Pseudomonas aeruginosa JCM 14847 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.1.1.294 methyltransf_25 domain-containing protein UniProt Pseudomonas aeruginosa
2.1.1.294 WarA
-
Pseudomonas aeruginosa
2.1.1.294 WbdD-like O antigen regulator
-
Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.294 S-adenosyl-L-methionine
-
Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
2.1.1.294 evolution WarA and WarB have structural similarities with the bi-functional Escherichia coli LPS O antigen regulator WbdD. A predicted three-dimensional model is generated with a very high confidence on the Escherichia coli serotype O9a WbdD protein (19% sequence identity, 69% sequence coverage, 100% confidence) Pseudomonas aeruginosa
2.1.1.294 malfunction The immune response to Pseudomonas aeruginosa in a Pseudomonas-zebrafish hindbrain infection model shows that deletion of warA or sadC does not reduce bacterial load. A phenotype of significantly increased neutrophil recruitment is observed for a warA deletion mutant. Infection with a warA mutant also induces TNF-alpha mRNA levels significantly higher than wild-type infected larvae Pseudomonas aeruginosa
2.1.1.294 additional information WarA is a methyltransferase in complex with a putative kinase WarB. WarA binds to cyclic-di-GMP, which potentiates its methyltransferase activity. Bacterial two-hybrid assay Pseudomonas aeruginosa
2.1.1.294 physiological function WarA influences Pseudomonas aeruginosa O antigen modal distribution and interacts with the LPS biogenesis machinery. LPS is known to modulate the immune response in the host, WarA is involved in the ability of Pseudomonas aeruginosa to evade detection by the host. Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion. WarA might play a similar functional role as WbdD in regulating chain length and termination of LPS O antigen synthesis. The immune response to Pseudomonas aeruginosa in a Pseudomonas-zebrafish hindbrain infection model shows that deletion of warA or sadC does not reduce bacterial load. The regulation of LPS by SadC and WarA plays a critical role in immune cell evasion during infection Pseudomonas aeruginosa