EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.5.3 | wild-type and mutant enzyme subunit gamma in complex with GTP, GDP, or GDP analogues, with Mg2+, X-ray diffraction structure determination and analysis at 1.3-1.94 A resolution | Saccharolobus solfataricus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.5.3 | D19A | site-directed mutagenesis, the mutation does not strongly modify the GDP binding properties of the two mutant enzyme, but reduces the GTP hydrolysis rate | Saccharolobus solfataricus |
3.6.5.3 | D19A/H97A | site-directed mutagenesis, almost inactive mutant | Saccharolobus solfataricus |
3.6.5.3 | H97A | site-directed mutagenesis, the mutation does not strongly modify the GDP binding properties of the two mutant enzyme, but reduces the GTP hydrolysis rate | Saccharolobus solfataricus |
3.6.5.3 | additional information | mutant gamma subunit structure determination and analysis, and comparison to the wild-type gamma subunit structure, GTP binding structures, overview | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.5.3 | additional information | - |
additional information | GTP hydrolysis kinetics of wild-type and mutant enzyme subunits | Saccharolobus solfataricus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.5.3 | ribosome | - |
Saccharolobus solfataricus | 5840 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.5.3 | Mg2+ | required | Saccharolobus solfataricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.5.3 | GTP + H2O | Saccharolobus solfataricus | GTP hydrolysis by subunit aIF2gamma | GDP + phosphate | - |
? | |
3.6.5.3 | GTP + H2O | Saccharolobus solfataricus P2 | GTP hydrolysis by subunit aIF2gamma | GDP + phosphate | - |
? | |
3.6.5.3 | GTP + H2O | Saccharolobus solfataricus JCM 11322 | GTP hydrolysis by subunit aIF2gamma | GDP + phosphate | - |
? | |
3.6.5.3 | GTP + H2O | Saccharolobus solfataricus ATCC 35092 | GTP hydrolysis by subunit aIF2gamma | GDP + phosphate | - |
? | |
3.6.5.3 | GTP + H2O | Saccharolobus solfataricus DSM 1617 | GTP hydrolysis by subunit aIF2gamma | GDP + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.5.3 | Saccharolobus solfataricus | Q980A5 | i.e. Sulfolobus solfataricus | - |
3.6.5.3 | Saccharolobus solfataricus ATCC 35092 | Q980A5 | i.e. Sulfolobus solfataricus | - |
3.6.5.3 | Saccharolobus solfataricus DSM 1617 | Q980A5 | i.e. Sulfolobus solfataricus | - |
3.6.5.3 | Saccharolobus solfataricus JCM 11322 | Q980A5 | i.e. Sulfolobus solfataricus | - |
3.6.5.3 | Saccharolobus solfataricus P2 | Q980A5 | i.e. Sulfolobus solfataricus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.5.3 | GTP + H2O | GTP hydrolysis by subunit aIF2gamma | Saccharolobus solfataricus | GDP + phosphate | - |
? | |
3.6.5.3 | GTP + H2O | aIF2 significantly hydrolyses GTP in vitro, GTP hydrolysis by aIF2 or by its isolated gamma subunit. Assay with aIF2-Met-tRNAfMet enzyme complex and GTP | Saccharolobus solfataricus | GDP + phosphate | - |
? | |
3.6.5.3 | GTP + H2O | GTP hydrolysis by subunit aIF2gamma | Saccharolobus solfataricus P2 | GDP + phosphate | - |
? | |
3.6.5.3 | GTP + H2O | aIF2 significantly hydrolyses GTP in vitro, GTP hydrolysis by aIF2 or by its isolated gamma subunit. Assay with aIF2-Met-tRNAfMet enzyme complex and GTP | Saccharolobus solfataricus P2 | GDP + phosphate | - |
? | |
3.6.5.3 | GTP + H2O | GTP hydrolysis by subunit aIF2gamma | Saccharolobus solfataricus JCM 11322 | GDP + phosphate | - |
? | |
3.6.5.3 | GTP + H2O | aIF2 significantly hydrolyses GTP in vitro, GTP hydrolysis by aIF2 or by its isolated gamma subunit. Assay with aIF2-Met-tRNAfMet enzyme complex and GTP | Saccharolobus solfataricus JCM 11322 | GDP + phosphate | - |
? | |
3.6.5.3 | GTP + H2O | GTP hydrolysis by subunit aIF2gamma | Saccharolobus solfataricus ATCC 35092 | GDP + phosphate | - |
? | |
3.6.5.3 | GTP + H2O | aIF2 significantly hydrolyses GTP in vitro, GTP hydrolysis by aIF2 or by its isolated gamma subunit. Assay with aIF2-Met-tRNAfMet enzyme complex and GTP | Saccharolobus solfataricus ATCC 35092 | GDP + phosphate | - |
? | |
3.6.5.3 | GTP + H2O | GTP hydrolysis by subunit aIF2gamma | Saccharolobus solfataricus DSM 1617 | GDP + phosphate | - |
? | |
3.6.5.3 | GTP + H2O | aIF2 significantly hydrolyses GTP in vitro, GTP hydrolysis by aIF2 or by its isolated gamma subunit. Assay with aIF2-Met-tRNAfMet enzyme complex and GTP | Saccharolobus solfataricus DSM 1617 | GDP + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.5.3 | heterotrimer | aIF2 is composed of three subunits, alpha, bbeta, and gamma. The gamma subunit forms the core of the heterotrimer and contains the GTP-binding pocket. alpha and beta are bound to subunit gamma but do not interact together. The gamma subunit closely resembles elongation factor EF1A | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.5.3 | aIF2 | - |
Saccharolobus solfataricus |
3.6.5.3 | aIF2-gamma | - |
Saccharolobus solfataricus |
3.6.5.3 | aIF2gamma | - |
Saccharolobus solfataricus |
3.6.5.3 | IF2 | - |
Saccharolobus solfataricus |
3.6.5.3 | initiation factor 2 | - |
Saccharolobus solfataricus |
3.6.5.3 | Ss-aIF2 | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.5.3 | 65 | - |
assay at | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.5.3 | 7.5 | - |
assay at | Saccharolobus solfataricus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.6.5.3 | additional information | in its GTP-bound form, aIF2 specifically binds Met-tRNAi Met and brings it to the initiation complex. The enzyme is active in its GTP-bound form, the GDP-bound form loses affinity for Met-tRNAi Met and eventually dissociates from the initiation complex. With EF1A, productive binding of tRNA is GTP-dependent and related to the ON conformations of two regions of the G domain called switch 1 and switch 2. Structure of the ternary initiation complex aIF2-GDPNP-Met-tRNA, molecular dynamics simulations, overview. Analysis of the nucleotide-binding pocket of Ss-aIF2gamma. QM/MM free energy simulations of the catalytic reaction | Saccharolobus solfataricus |
3.6.5.3 | physiological function | archaeal translation initiation processes, like eukaryotic, involve a heterotrimeric GTPase aIF2 (eIF2) crucial for accuracy of start codon selection. Enzyme aIF2 is peculiar in that it functions on the small ribosomal subunit, whereas other translational GTPases bind the same region of the assembled ribosome in all species and likely use the sarcin-ricin loop in the large subunit for activation of GTP hydrolysis | Saccharolobus solfataricus |