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Literature summary extracted from

  • Wilding, M.; Scott, C.; Newman, J.; Peat, T.S.
    Crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC (2017), Acta Crystallogr. Sect. F, 73, 29-35 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.82 recombinant His-tagged enzyme expression in Escherichia coli strain Rosetta 2 (DE3) as soluble protein Pseudomonas sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.82 purified recombinant His-tagged enzyme, sitting drop vapour diffusion metod, mixing of 150 nl of 10.5 mg/ml protein in 25 mM Tris chloride, 137 mM NaCl, and 3 mM KCl, pH 8.0, with 150 nl of reservoir solution containing 0.066 M calcium acetate, 18% w/v PEG MME 5000, 0.1 M Tris chloride, pH 7.6, and equilibration against 0.5 ml of reservoir solution, at 20°C, method optimization, X-ray diffraction structure determination analysis at 2.07 A resolution, molecular replacement method, structure modelling using the structure of a Chromobacterium violaceum omega transaminase monomer (PDB ID 4a6t) as the search model Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.82 gabaculine a transaminase suicide inhibitor Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.82 putrescine + 2-oxoglutarate Pseudomonas sp.
-
1-pyrroline + L-glutamate + H2O
-
?
2.6.1.82 putrescine + 2-oxoglutarate Pseudomonas sp. AAC
-
1-pyrroline + L-glutamate + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.82 Pseudomonas sp.
-
-
-
2.6.1.82 Pseudomonas sp. AAC
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.82 recombinant soluble His-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) microfluidized cell extract by nickel affinity chromatography, gel filtration, and ultrafiltration Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.82 12-aminododecanoic acid + 2-oxoglutarate
-
Pseudomonas sp. ?
-
?
2.6.1.82 12-aminododecanoic acid + 2-oxoglutarate
-
Pseudomonas sp. AAC ?
-
?
2.6.1.82 additional information the activity of KES24511 is determined using an alanine dehydrogenase enzyme-coupled assay, the purified protein exhibited activity with two substrates, putrescine and 12-aminododecanoic acid, with pyruvate as the co-substrate for transamination. The transaminase reaction co-product (L-alanine) is converted in situ by the dehydrogenase in an NAD+-dependent oxidation, and nicotinamide conversion is spectrophotometrically monitored by the change in UV absorbance at 340 nm Pseudomonas sp. ?
-
-
2.6.1.82 additional information the activity of KES24511 is determined using an alanine dehydrogenase enzyme-coupled assay, the purified protein exhibited activity with two substrates, putrescine and 12-aminododecanoic acid, with pyruvate as the co-substrate for transamination. The transaminase reaction co-product (L-alanine) is converted in situ by the dehydrogenase in an NAD+-dependent oxidation, and nicotinamide conversion is spectrophotometrically monitored by the change in UV absorbance at 340 nm Pseudomonas sp. AAC ?
-
-
2.6.1.82 putrescine + 2-oxoglutarate
-
Pseudomonas sp. 1-pyrroline + L-glutamate + H2O
-
?
2.6.1.82 putrescine + 2-oxoglutarate overall reaction Pseudomonas sp. 1-pyrroline + L-glutamate + H2O
-
?
2.6.1.82 putrescine + 2-oxoglutarate
-
Pseudomonas sp. AAC 1-pyrroline + L-glutamate + H2O
-
?
2.6.1.82 putrescine + 2-oxoglutarate overall reaction Pseudomonas sp. AAC 1-pyrroline + L-glutamate + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.82 FG99_07980
-
Pseudomonas sp.
2.6.1.82 KES24511
-
Pseudomonas sp.
2.6.1.82 putrescine aminotransferase
-
Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.82 62.3
-
Tm of KES24511 in 50 mM Tris chloride, pH 8.0, 200 mM NaCl Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.82 pyridoxal 5'-phosphate PLP, the the PLP cofactor is typically covalently bound in the active site to a lysine residue in an internal aldimine conformation Pseudomonas sp.

General Information

EC Number General Information Comment Organism
2.6.1.82 metabolism the enzyme KES24511 is involved in the amino alkanoate catabolism of the 12-aminododecanoic acid-metabolizing Pseudomonas sp. strain AAC Pseudomonas sp.