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Literature summary extracted from

  • Ramirez-Estrada, K.; Castillo, N.; Lara, J.A.; Arro, M.; Boronat, A.; Ferrer, A.; Altabella, T.
    Tomato UDP-glucose sterol glycosyltransferases a family of developmental and stress regulated genes that encode cytosolic and membrane-associated forms of the enzyme (2017), Front. Plant Sci., 8, 984 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.173 gene SGT1, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1 Solanum lycopersicum
2.4.1.173 gene SGT2, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1 Solanum lycopersicum
2.4.1.173 gene SGT3, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1 Solanum lycopersicum
2.4.1.173 gene SGT4, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1 Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.173 cytosol
-
 Solanum lycopersicum 5829
-
2.4.1.173 additional information SGT isozymes from Solanum lycopersicum are located in cytosol and/or plasma membrane, overview Solanum lycopersicum
-
-
2.4.1.173 plasma membrane
-
 Solanum lycopersicum 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.173 UDP-glucose + a sterol Solanum lycopersicum
-
 UDP + a sterol 3-beta-D-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Solanum lycopersicum A0A2D1N4Z8 cv. Micro-Tom
-
2.4.1.173 Solanum lycopersicum K4BS77 cv. Micro-Tom
-
2.4.1.173 Solanum lycopersicum K4BTE6 cv. Micro-Tom
-
2.4.1.173 Solanum lycopersicum K4C3H8 cv. Micro-Tom
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.173 flower
-
 Solanum lycopersicum
-
2.4.1.173 fruit
-
 Solanum lycopersicum
-
2.4.1.173 fruit expression of SlSGT3 is clearly different from other isozymes since its mRNA levels are low in developing fruits (small green and mature green stages) but increase sharply when fruits start to ripe and remain at similar high levels until the red mature stage Solanum lycopersicum
-
2.4.1.173 fruit in small green, mature green, and mature red fruits. SlSGT1 mRNA levels decrease when fruits start to ripe (breaker and orange stages) but increase again in red ripe fruits Solanum lycopersicum
-
2.4.1.173 fruit in small green, mature green, and mature red fruits. SlSGT2 mRNA levels decrease when fruits start to ripe (breaker and orange stages) but increase again in red ripe fruits Solanum lycopersicum
-
2.4.1.173 leaf
-
 Solanum lycopersicum
-
2.4.1.173 additional information the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening Solanum lycopersicum
-
2.4.1.173 additional information the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT1 is the most intensely expressed in root, leaf, and flower tissues, being roots and leaves the tissues where the highest levels of SlSGT1 mRNA are detected. SlSGT1 is again the most actively expressed SlSGT gene in both developing (small green and mature green stages) and mature red fruits Solanum lycopersicum
-
2.4.1.173 additional information the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT4 transcripts are barely detectable in all samples analyzed. Solanum lycopersicum
-
2.4.1.173 root
-
 Solanum lycopersicum
-
2.4.1.173 seedling
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 additional information SGT activity assays are carried out with a plant sterol mixture consisting of approximately 13% brassicasterol, 26% campesterol, 7% stigmasterol, and 53% beta-sitosterol in ethanol, and UDP-glucose Solanum lycopersicum ?
-
-
2.4.1.173 UDP-glucose + a sterol
-
 Solanum lycopersicum UDP + a sterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + beta-sitosterol
-
 Solanum lycopersicum UDP + beta-sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + brassicasterol
-
 Solanum lycopersicum UDP + brassicasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + campesterol
-
 Solanum lycopersicum UDP + campesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol
-
 Solanum lycopersicum UDP + stigmasterol 3-beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.173 SGT1
-
 Solanum lycopersicum
2.4.1.173 SGT2
-
 Solanum lycopersicum
2.4.1.173 SGT3
-
 Solanum lycopersicum
2.4.1.173 SGT4
-
 Solanum lycopersicum
2.4.1.173 SlSGT1
-
 Solanum lycopersicum
2.4.1.173 SlSGT2
-
 Solanum lycopersicum
2.4.1.173 SlSGT3
-
 Solanum lycopersicum
2.4.1.173 SlSGT4
-
 Solanum lycopersicum
2.4.1.173 Solyc04g051150.2.1 locus name Solanum lycopersicum
2.4.1.173 Solyc04g071540.2.1 locus name Solanum lycopersicum
2.4.1.173 Solyc06g007980.2.1 locus name Solanum lycopersicum
2.4.1.173 Solyc09g061860.2.1 locus name Solanum lycopersicum
2.4.1.173 UDP-glucose sterol glycosyltransferase
-
 Solanum lycopersicum
2.4.1.173 UDP-glucose:sterol glucosyltransferase
-
 Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.173 30
-
 assay at Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.173 8
-
 assay at Solanum lycopersicum

Expression

EC Number Organism Comment Expression
2.4.1.173 Solanum lycopersicum transcriptional profiling of SlSGT gene expression in response to stress, overview additional information
2.4.1.173 Solanum lycopersicum transcriptional profiling of SlSGT gene expression in response to stress, overview. SlSGT1 expression remains almost unaltered under the tested stress conditions additional information
2.4.1.173 Solanum lycopersicum transcriptional profiling of SlSGT gene expression in response to stress, overview. SlSGT3 expression remains almost unaltered under the tested stress conditions additional information
2.4.1.173 Solanum lycopersicum SlSGT2 expression increases markedly in response to osmotic, salt, and cold stress, as well as upon treatment with abscisic acid and methyl jasmonate up
2.4.1.173 Solanum lycopersicum SlSGT4 expression increases markedly in response to osmotic, salt, and cold stress, as well as upon treatment with abscisic acid and methyl jasmonate up

General Information

EC Number General Information Comment Organism
2.4.1.173 evolution the enzyme belongs to the UDP-glucose sterol glycosyltransferases family of developmental and stress regulated genes that encode cytosolic and membrane-associated forms of the enzyme. Identification and functional characterization of the four members, SlSGT1-4, of the tomato cv. Micro-Tom Solanum lycopersicum
2.4.1.173 physiological function sterol glycosyltransferases (SGTs) catalyze the glycosylation of the free hydroxyl group at C-3 position of sterols to produce sterol glycosides. Solanum contain very high levels of glycosylated sterols, which in the case of tomato may account for more than 85% of the total sterol content. Tomato SGT isozymes show the ability to glycosylate different sterol species including cholesterol, brassicasterol, campesterol, stigmasterol, and beta-sitosterol, which is consistent with the occurrence in their primary structure of the putative steroid-binding domain found in steroid UDP-glucuronosyltransferases and the UDP-sugar binding domain characteristic for a superfamily of nucleoside diphosphosugar glycosyltransferases. The SlSGT isozyme are differentially regulated in response to biotic and abiotic stress conditions. Changes in the relative proportions of sterols alter membrane fluidity and permeability and hence regulate different membrane functions such as simple and carrier-mediated diffusion, active transport across the membrane, and the activity of membrane-associated proteins. Tomato SGT isozymes play overlapping but not completely redundant biological functions involved in mediating developmental and stress responses Solanum lycopersicum
2.4.1.173 physiological function sterol glycosyltransferases (SGTs) catalyze the glycosylation of the free hydroxyl group at C-3 position of sterols to produce sterol glycosides. Solanum contain very high levels of glycosylated sterols, which in the case of tomato may account for more than 85% of the total sterol content. Tomato SGT isozymes show the ability to glycosylate different sterol species including cholesterol, brassicasterol, campesterol, stigmasterol, and beta-sitosterol, which is consistent with the occurrence in their primary structure of the putative steroid-binding domain found in steroid UDP-glucuronosyltransferases and the UDP-sugar binding domain characteristic for a superfamily of nucleoside diphosphosugar glycosyltransferases. The SlSGT isozyme are differentially regulated in response to biotic and abiotic stress conditions. Tomato SGT isozymes play overlapping but not completely redundant biological functions involved in mediating developmental and stress responses. Changes in the relative proportions of sterols alter membrane fluidity and permeability and hence regulate different membrane functions such as simple and carrier-mediated diffusion, active transport across the membrane, and the activity of membrane-associated proteins Solanum lycopersicum