EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.18 | expressed in Escherichia coli BL21 Silex cells | Drosophila melanogaster |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.18 | hanging drop vapor diffusion method, using 0.1M succinic acid-phosphate-glycine buffer with 25% (w/v) PEG 1500 at pH 4.0 | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.18 | allyl-isothiocyanate | about 30% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | Berberine | complete inhibition at 0.1 mM | Drosophila melanogaster | |
2.5.1.18 | Caffeine | about 25% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | chrysin | about 30% inhibition at 0.1 mM | Drosophila melanogaster | |
2.5.1.18 | coumarin | about 17% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | denatonium benzoate | about 10% inhibition at 0. 1 mM | Drosophila melanogaster | |
2.5.1.18 | Digitonin | about 20% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | escin | about 10% inhibition at 0.1 mM | Drosophila melanogaster | |
2.5.1.18 | fisetin | 60% inhibition at 0.1 mM | Drosophila melanogaster | |
2.5.1.18 | flavonol | about 20% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | kaempferol | about 42% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | luteolin | about 16% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | naringenin | 42% inhibition at 0.1 mM | Drosophila melanogaster | |
2.5.1.18 | naringin | about 17% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | Nicotine | about 16% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | papaverine | about 5% inhibition at 0.1 mM | Drosophila melanogaster | |
2.5.1.18 | paraxanthine | about 9% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | phenethyl-isothiocyanate | about 25% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | piperidine | about 15% inhibition at 0.1 mM | Drosophila melanogaster | |
2.5.1.18 | propyl-isothiocyanate | 42% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | Pyridine | about 17% inhibition at 0.1 mM | Drosophila melanogaster | |
2.5.1.18 | quercetin | complete inhibition at 0.1 mM, 52% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | Quinine | about 18% inhibition at 0.1 mM | Drosophila melanogaster | |
2.5.1.18 | sinigrin hydrate | about 28% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | strychnine | about 37% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | tangeretin | complete inhibition at 0.1 mM | Drosophila melanogaster | |
2.5.1.18 | taurine | about 19% inhibition at 0.1 mM | Drosophila melanogaster | |
2.5.1.18 | Theobromine | about 12% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | theophylline | about 10% inhibition at 0.01 mM | Drosophila melanogaster | |
2.5.1.18 | thiamine hydrochloride | about 5% inhibition at 0.1 mM | Drosophila melanogaster | |
2.5.1.18 | thiazolidine | about 18% inhibition at 0.01 mM | Drosophila melanogaster |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.18 | 49850 | - |
SEC-MALS analysis | Drosophila melanogaster |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.18 | Drosophila melanogaster | Q9VG98 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.18 | GSTrap column chromatography | Drosophila melanogaster |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.18 | antenna | - |
Drosophila melanogaster | - |
2.5.1.18 | foreleg | - |
Drosophila melanogaster | - |
2.5.1.18 | head | - |
Drosophila melanogaster | - |
2.5.1.18 | maxillary palp | - |
Drosophila melanogaster | - |
2.5.1.18 | olfactory organ | - |
Drosophila melanogaster | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.18 | 8 | - |
with propyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication | Drosophila melanogaster |
2.5.1.18 | 36 | - |
with allyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication | Drosophila melanogaster |
2.5.1.18 | 74 | - |
with phenethyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication | Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.18 | glutathione + 1-chloro-2,4-dinitrobenzene | - |
Drosophila melanogaster | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
2.5.1.18 | glutathione + allyl-isothiocyanate | - |
Drosophila melanogaster | ? | - |
? | |
2.5.1.18 | glutathione + phenethyl-isothiocyanate | - |
Drosophila melanogaster | ? | - |
? | |
2.5.1.18 | glutathione + propyl-isothiocyanate | - |
Drosophila melanogaster | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.18 | homodimer | 2 * 25000, SDS-PAGE | Drosophila melanogaster |
2.5.1.18 | homodimer | 2 * 24535, calculated from amino acid sequence | Drosophila melanogaster |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.18 | GST Delta 2 | isoform | Drosophila melanogaster |
2.5.1.18 | GSTD2 | isoform | Drosophila melanogaster |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.5.1.18 | Drosophila melanogaster | in the olfactory organs, the GSTD2 mRNA level is strongly decreased (5.9fold) in the presence of phenethyl-isothiocyanate | down |
2.5.1.18 | Drosophila melanogaster | isoform GSTD2 expression levels increase in the presence of propyl-, phenethyl- and allyl-isothiocyanates with phenethyl-isothiocyanate (0.25 mM) inducing the highest effect (6fold increase). Phenethyl-isothiocyanate highly induces GSTD2 mRNA expression in the taste organs (3.5fold) | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.18 | physiological function | the enzyme plays a biological role in chemoperception | Drosophila melanogaster |