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Literature summary extracted from

  • Gonzalez, D.; Fraichard, S.; Grassein, P.; Delarue, P.; Senet, P.; Nicolai, A.; Chavanne, E.; Mucher, E.; Artur, Y.; Ferveur, J.F.; Heydel, J.M.; Briand, L.; Neiers, F.
    Characterization of a Drosophila glutathione transferase involved in isothiocyanate detoxification (2018), Insect Biochem. Mol. Biol., 95, 33-43 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli BL21 Silex cells Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.18 hanging drop vapor diffusion method, using 0.1M succinic acid-phosphate-glycine buffer with 25% (w/v) PEG 1500 at pH 4.0 Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 allyl-isothiocyanate about 30% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 Berberine complete inhibition at 0.1 mM Drosophila melanogaster
2.5.1.18 Caffeine about 25% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 chrysin about 30% inhibition at 0.1 mM Drosophila melanogaster
2.5.1.18 coumarin about 17% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 denatonium benzoate about 10% inhibition at 0. 1 mM Drosophila melanogaster
2.5.1.18 Digitonin about 20% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 escin about 10% inhibition at 0.1 mM Drosophila melanogaster
2.5.1.18 fisetin 60% inhibition at 0.1 mM Drosophila melanogaster
2.5.1.18 flavonol about 20% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 kaempferol about 42% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 luteolin about 16% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 naringenin 42% inhibition at 0.1 mM Drosophila melanogaster
2.5.1.18 naringin about 17% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 Nicotine about 16% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 papaverine about 5% inhibition at 0.1 mM Drosophila melanogaster
2.5.1.18 paraxanthine about 9% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 phenethyl-isothiocyanate about 25% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 piperidine about 15% inhibition at 0.1 mM Drosophila melanogaster
2.5.1.18 propyl-isothiocyanate 42% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 Pyridine about 17% inhibition at 0.1 mM Drosophila melanogaster
2.5.1.18 quercetin complete inhibition at 0.1 mM, 52% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 Quinine about 18% inhibition at 0.1 mM Drosophila melanogaster
2.5.1.18 sinigrin hydrate about 28% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 strychnine about 37% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 tangeretin complete inhibition at 0.1 mM Drosophila melanogaster
2.5.1.18 taurine about 19% inhibition at 0.1 mM Drosophila melanogaster
2.5.1.18 Theobromine about 12% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 theophylline about 10% inhibition at 0.01 mM Drosophila melanogaster
2.5.1.18 thiamine hydrochloride about 5% inhibition at 0.1 mM Drosophila melanogaster
2.5.1.18 thiazolidine about 18% inhibition at 0.01 mM Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 49850
-
SEC-MALS analysis Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Drosophila melanogaster Q9VG98
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 GSTrap column chromatography Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 antenna
-
Drosophila melanogaster
-
2.5.1.18 foreleg
-
Drosophila melanogaster
-
2.5.1.18 head
-
Drosophila melanogaster
-
2.5.1.18 maxillary palp
-
Drosophila melanogaster
-
2.5.1.18 olfactory organ
-
Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 8
-
with propyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication Drosophila melanogaster
2.5.1.18 36
-
with allyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication Drosophila melanogaster
2.5.1.18 74
-
with phenethyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
Drosophila melanogaster chloride + 2,4-dinitrophenyl-glutathione
-
?
2.5.1.18 glutathione + allyl-isothiocyanate
-
Drosophila melanogaster ?
-
?
2.5.1.18 glutathione + phenethyl-isothiocyanate
-
Drosophila melanogaster ?
-
?
2.5.1.18 glutathione + propyl-isothiocyanate
-
Drosophila melanogaster ?
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 homodimer 2 * 25000, SDS-PAGE Drosophila melanogaster
2.5.1.18 homodimer 2 * 24535, calculated from amino acid sequence Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GST Delta 2 isoform Drosophila melanogaster
2.5.1.18 GSTD2 isoform Drosophila melanogaster

Expression

EC Number Organism Comment Expression
2.5.1.18 Drosophila melanogaster in the olfactory organs, the GSTD2 mRNA level is strongly decreased (5.9fold) in the presence of phenethyl-isothiocyanate down
2.5.1.18 Drosophila melanogaster isoform GSTD2 expression levels increase in the presence of propyl-, phenethyl- and allyl-isothiocyanates with phenethyl-isothiocyanate (0.25 mM) inducing the highest effect (6fold increase). Phenethyl-isothiocyanate highly induces GSTD2 mRNA expression in the taste organs (3.5fold) up

General Information

EC Number General Information Comment Organism
2.5.1.18 physiological function the enzyme plays a biological role in chemoperception Drosophila melanogaster