Literature summary extracted from
Okamura-Ikeda, K.; Fujiwara, K.; Motokawa, Y.
Purification and characterization of chicken liver T-protein, a component of the glycine cleavage system (1982), J. Biol. Chem., 257, 135-139 .
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.4.1.27 |
0.17 |
- |
tetrahydrofolate |
pH 8.0, 37°C |
Gallus gallus |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.4.1.27 |
37000 |
- |
sedimentation equilibrium centrifugation, component T-protein |
Gallus gallus |
1.4.1.27 |
38000 |
- |
gel filtration, component T-protein |
Gallus gallus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.4.1.27 |
Gallus gallus |
P28337 |
i.e. aminomethyltransferase component T-protein, cf. EC 2.1.2.10 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.4.1.27 |
isolation of component T-protein |
Gallus gallus |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.4.1.27 |
glycine + tetrahydrofolate + NAD+ |
- |
Gallus gallus |
5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.4.1.27 |
multimer |
x * 41000, SDS-PAGE, component T-protein |
Gallus gallus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.4.1.27 |
NAD+ |
- |
Gallus gallus |
|
1.4.1.27 |
tetrahydrofolate |
- |
Gallus gallus |
|
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
1.4.1.27 |
Gallus gallus |
component T-protein |
- |
9.8 |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
1.4.1.27 |
physiological function |
the component T-protein catalyzes the degradation of the protein-bound intermediate (-CH2NH2 moiety of glycine) to a 1-carbon unit and NH3. The reaction is dependent on tetrahydrofolate. T-protein associates with H-protein forming a complex of one molecule each of T-protein and H-protein |
Gallus gallus |