EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.4 | gene BDH1, cloning from Saccharomyces cerevisiae strains YBA_08 (denoted Y) and BY4742 (denoted B), DNA and amino acid sequence determination and analysis and phylogenetic tree analysis, subcloning in to Escherichia coli strain DH5alpha and functional overexpression in Saccharomyces cerevisiae strain INVSc1, recombinant expression of His-tagged enzymes originnating from both strains | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.4 | 0.65 | - |
acetaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B | Saccharomyces cerevisiae | |
1.1.1.4 | 0.67 | - |
acetaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B | Saccharomyces cerevisiae | |
1.1.1.4 | 4.49 | - |
acetaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y | Saccharomyces cerevisiae | |
1.1.1.4 | 9.38 | - |
glycolaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y | Saccharomyces cerevisiae | |
1.1.1.4 | 15.52 | - |
glycolaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y | Saccharomyces cerevisiae | |
1.1.1.4 | 17.91 | - |
glycolaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B | Saccharomyces cerevisiae | |
1.1.1.4 | 20.18 | - |
acetaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y | Saccharomyces cerevisiae | |
1.1.1.4 | 29.42 | - |
glycolaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B | Saccharomyces cerevisiae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.4 | Zn2+ | the enzyme has a catalytic zinc binding domain | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | Saccharomyces cerevisiae | - |
(R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | Saccharomyces cerevisiae YBA_08 | - |
(R)-acetoin + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.4 | Saccharomyces cerevisiae | A0A7G4NVP1 | strain Y/YBA_08 | - |
1.1.1.4 | Saccharomyces cerevisiae | A0A7G4NVP2 | strain Y/YBA_08 | - |
1.1.1.4 | Saccharomyces cerevisiae YBA_08 | A0A7G4NVP1 | strain Y/YBA_08 | - |
1.1.1.4 | Saccharomyces cerevisiae YBA_08 | A0A7G4NVP2 | strain Y/YBA_08 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.4 | recombinant His-tagged enzyme by nickel affinity chromatography | Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 0.24 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates glycolaldehyde and NADPH | Saccharomyces cerevisiae |
1.1.1.4 | 0.48 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates formaldehyde and NADPH | Saccharomyces cerevisiae |
1.1.1.4 | 0.76 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates formaldehyde and NADPH | Saccharomyces cerevisiae |
1.1.1.4 | 0.82 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates furfural and NADPH | Saccharomyces cerevisiae |
1.1.1.4 | 1.16 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates hexaldehyde and NADH | Saccharomyces cerevisiae |
1.1.1.4 | 1.3 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates formaldehyde and NADH | Saccharomyces cerevisiae |
1.1.1.4 | 1.71 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates formaldehyde and NADH | Saccharomyces cerevisiae |
1.1.1.4 | 1.94 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates propionaldehyde and NADH | Saccharomyces cerevisiae |
1.1.1.4 | 2.47 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates hexaldehyde and NADH | Saccharomyces cerevisiae |
1.1.1.4 | 2.72 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates acetaldehyde and NADPH | Saccharomyces cerevisiae |
1.1.1.4 | 2.74 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates furfural and NADH | Saccharomyces cerevisiae |
1.1.1.4 | 3.44 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates acetaldehyde and NADPH | Saccharomyces cerevisiae |
1.1.1.4 | 4.95 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates furfural and NADH | Saccharomyces cerevisiae |
1.1.1.4 | 6.27 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates glycolaldehyde and NADH | Saccharomyces cerevisiae |
1.1.1.4 | 10.2 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates glycolaldehyde and NADH | Saccharomyces cerevisiae |
1.1.1.4 | 78.58 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates acetaldehyde and NADH | Saccharomyces cerevisiae |
1.1.1.4 | 117.95 | - |
purified recombinant enzyme, pH 7.0, 30°C, substrates acetaldehyde and NADH | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | - |
Saccharomyces cerevisiae | (R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | - |
Saccharomyces cerevisiae YBA_08 | (R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | acetaldehyde + NADH + H+ | - |
Saccharomyces cerevisiae | ethanol + NAD+ | - |
? | |
1.1.1.4 | acetaldehyde + NADH + H+ | - |
Saccharomyces cerevisiae YBA_08 | ethanol + NAD+ | - |
? | |
1.1.1.4 | acetaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ethanol + NADP+ | - |
? | |
1.1.1.4 | acetaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae YBA_08 | ethanol + NADP+ | - |
? | |
1.1.1.4 | formaldehyde + NADH + H+ | - |
Saccharomyces cerevisiae | ? + NAD+ | - |
? | |
1.1.1.4 | formaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
1.1.1.4 | furfural + NADH + H+ | - |
Saccharomyces cerevisiae | (furan-2-yl)methanol + NAD+ | - |
? | |
1.1.1.4 | furfural + NADH + H+ | - |
Saccharomyces cerevisiae YBA_08 | (furan-2-yl)methanol + NAD+ | - |
? | |
1.1.1.4 | furfural + NADPH + H+ | - |
Saccharomyces cerevisiae | (furan-2-yl)methanol + NADP+ | - |
? | |
1.1.1.4 | glycolaldehyde + NADH + H+ | - |
Saccharomyces cerevisiae | ? + NAD+ | - |
? | |
1.1.1.4 | hexaldehyde + NADH + H+ | - |
Saccharomyces cerevisiae | ? + NAD+ | - |
? | |
1.1.1.4 | additional information | enzyme Bdh1p exhibits (2R,3R)-2,3-butanediol dehydrogenase activity, but Bdh1p also exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural. No activity for glycolaldehyde or hexaldehyde with NADPH. Propionaldehyde is a poor substrate, no activity with glutaraldehyde | Saccharomyces cerevisiae | ? | - |
- |
|
1.1.1.4 | additional information | enzyme Bdh1p exhibits (2R,3R)-2,3-butanediol dehydrogenase activity, but Bdh2p also exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural. No activity for furfural, propionaldehyde, or hexaldehyde with NADPH. Glutaraldehyde is a poor substrate | Saccharomyces cerevisiae | ? | - |
- |
|
1.1.1.4 | additional information | enzyme Bdh1p exhibits (2R,3R)-2,3-butanediol dehydrogenase activity, but Bdh1p also exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural. No activity for glycolaldehyde or hexaldehyde with NADPH. Propionaldehyde is a poor substrate, no activity with glutaraldehyde | Saccharomyces cerevisiae YBA_08 | ? | - |
- |
|
1.1.1.4 | additional information | enzyme Bdh1p exhibits (2R,3R)-2,3-butanediol dehydrogenase activity, but Bdh2p also exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural. No activity for furfural, propionaldehyde, or hexaldehyde with NADPH. Glutaraldehyde is a poor substrate | Saccharomyces cerevisiae YBA_08 | ? | - |
- |
|
1.1.1.4 | propionaldehyde + NADH + H+ | - |
Saccharomyces cerevisiae | ? + NAD+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.4 | (2R,3R)-2,3-butanediol dehydrogenase | - |
Saccharomyces cerevisiae |
1.1.1.4 | BDH | - |
Saccharomyces cerevisiae |
1.1.1.4 | BDH1 | - |
Saccharomyces cerevisiae |
1.1.1.4 | Bdh1p | - |
Saccharomyces cerevisiae |
1.1.1.4 | BDH2 | - |
Saccharomyces cerevisiae |
1.1.1.4 | Bdh2p | - |
Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 35 | 45 | aldehyde reduction activity | Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.4 | 7.39 | - |
glycolaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B | Saccharomyces cerevisiae | |
1.1.1.4 | 9.38 | - |
acetaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B | Saccharomyces cerevisiae | |
1.1.1.4 | 9.43 | - |
glycolaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B | Saccharomyces cerevisiae | |
1.1.1.4 | 10.03 | - |
acetaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B | Saccharomyces cerevisiae | |
1.1.1.4 | 37.32 | - |
glycolaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y | Saccharomyces cerevisiae | |
1.1.1.4 | 53.46 | - |
glycolaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y | Saccharomyces cerevisiae | |
1.1.1.4 | 56.57 | - |
acetaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y | Saccharomyces cerevisiae | |
1.1.1.4 | 96.62 | - |
acetaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 6.5 | 7.5 | aldehyde reduction activity | Saccharomyces cerevisiae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 4.5 | 9 | activities of Bdh1p(Y) and Bdh1p(B) in glucoladehyde reduction gradually drop to approximately 50% at pH 4.5-7.0 after 6 h incubation. Especially, Bdh1p(Y) still retains 80% of its activity at pH 4.5 after incubation for 3 h. At alkaline conditions (pH 8.0-9.0), activities of the two enzymes drop rapidly to 28-45% within the first 3 h, and 3 more hours later, the lowest activity of Bdh1p(Y) is 17% at pH 9.0 | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.4 | NAD+ | - |
Saccharomyces cerevisiae | |
1.1.1.4 | NADH | - |
Saccharomyces cerevisiae | |
1.1.1.4 | NADPH | - |
Saccharomyces cerevisiae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.4 | evolution | two (2R,3R)-2,3-butanediol dehydrogenases (BDHs) from industrial (denoted Y)/laboratory (denoted B) strains of Saccharomyces cerevisiae, Bdh1p(Y)/Bdh1p(B) and Bdh2p(Y)/Bdh2p(B), are members of the PDH subfamily with an NAD(P)H binding domain and a catalytic zinc binding domain, and exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural | Saccharomyces cerevisiae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.4 | 0.32 | - |
glycolaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B | Saccharomyces cerevisiae | |
1.1.1.4 | 0.41 | - |
glycolaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B | Saccharomyces cerevisiae | |
1.1.1.4 | 2.41 | - |
glycolaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y | Saccharomyces cerevisiae | |
1.1.1.4 | 4.79 | - |
acetaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y | Saccharomyces cerevisiae | |
1.1.1.4 | 5.7 | - |
glycolaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y | Saccharomyces cerevisiae | |
1.1.1.4 | 12.6 | - |
acetaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y | Saccharomyces cerevisiae | |
1.1.1.4 | 14 | - |
acetaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B | Saccharomyces cerevisiae | |
1.1.1.4 | 15.43 | - |
acetaldehyde | recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B | Saccharomyces cerevisiae |