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Literature summary extracted from

  • Jo, J.H.; Park, Y.C.; Jin, Y.S.; Seo, J.H.
    Construction of efficient xylose-fermenting Saccharomyces cerevisiae through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis (2017), Biores. Technol., 241, 88-94 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.9 gene xyl2, the xylose-metabolic gene XYL2 from Scheffersomyces stipitis is introduced into Saccharomyces cerevisiae Scheffersomyces stipitis
1.1.1.307 gene xyl1, the xylose-metabolic gene XYL1 from Scheffersomyces stipitis are introduced into Saccharomyces cerevisiae Scheffersomyces stipitis
2.7.1.17 gene xyl3, the xylose-metabolic gene XYL3 from Scheffersomyces stipitis is introduced into Saccharomyces cerevisiae Scheffersomyces stipitis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.9 additional information construction of efficient xylose-fermenting Saccharomyces cerevisiae strain DXS through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis. The xylose-metabolic genes XYL1 (EC 1.1.1.307), XYL2, and XYL3 (EC 2.7.1.17) from Scheffersomyces stipitis are introduced into Saccharomyces cerevisiae. Construction of control strains SR6 and MM through random integration of the XR (NADPH) or mXR (NADH) expression cassette, respectively, at the multipled elements of the genome of Saccharomyces cerevisiae strain D452-2. Fermentation parameters of engineered Saccharomyces cerevisiae strains in mixed sugar fermentations (glucose and xylose), overview Scheffersomyces stipitis
1.1.1.307 additional information construction of efficient xylose-fermenting Saccharomyces cerevisiae strain DXS through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis. The xylose-metabolic genes XYL1, XYL2 (EC 1.1.1.9), and XYL3 (EC 2.7.1.17) from Scheffersomyces stipitis are introduced into Saccharomyces cerevisiae. Construction of control strains SR6 and MM through random integration of the XR (NADPH) or mXR (NADH) expression cassette, respectively, at the multipled elements of the genome of Saccharomyces cerevisiae strain D452-2. Fermentation parameters of engineered Saccharomyces cerevisiae strains in mixed sugar fermentations (glucose and xylose), overview Scheffersomyces stipitis
2.7.1.17 additional information construction of efficient xylose-fermenting Saccharomyces cerevisiae strain DXS through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis. The xylose-metabolic genes XYL1 (EC 1.1.1.307), XYL2 (EC 1.1.1.9), and XYL3 from Scheffersomyces stipitis are introduced into Saccharomyces cerevisiae. Construction of control strains SR6 and MM through random integration of the XR (NADPH) or mXR (NADH) expression cassette, respectively, at the multipled elements of the genome of Saccharomyces cerevisiae strain D452-2. Fermentation parameters of engineered Saccharomyces cerevisiae strains in mixed sugar fermentations (glucose and xylose), overview Scheffersomyces stipitis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.17 Mg2+ required Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.9 xylitol + NAD+ Scheffersomyces stipitis
-
 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Scheffersomyces stipitis NRRL Y-11545
-
 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Scheffersomyces stipitis NBRC 10063
-
 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Scheffersomyces stipitis ATCC 58785
-
 D-xylulose + NADH + H+
-
 r
1.1.1.307 xylitol + NAD(P)+ Scheffersomyces stipitis
-
 D-xylose + NAD(P)H + H+
-
 r
1.1.1.307 xylitol + NAD(P)+ Scheffersomyces stipitis NRRL Y-11545
-
 D-xylose + NAD(P)H + H+
-
 r
1.1.1.307 xylitol + NAD(P)+ Scheffersomyces stipitis NBRC 10063
-
 D-xylose + NAD(P)H + H+
-
 r
1.1.1.307 xylitol + NAD(P)+ Scheffersomyces stipitis ATCC 58785
-
 D-xylose + NAD(P)H + H+
-
 r
2.7.1.17 ATP + D-xylulose Scheffersomyces stipitis
-
 ADP + D-xylulose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Scheffersomyces stipitis P22144
-
-
1.1.1.9 Scheffersomyces stipitis ATCC 58785 P22144
-
-
1.1.1.9 Scheffersomyces stipitis NBRC 10063 P22144
-
-
1.1.1.9 Scheffersomyces stipitis NRRL Y-11545 P22144
-
-
1.1.1.307 Scheffersomyces stipitis P31867
-
-
1.1.1.307 Scheffersomyces stipitis ATCC 58785 P31867
-
-
1.1.1.307 Scheffersomyces stipitis NBRC 10063 P31867
-
-
1.1.1.307 Scheffersomyces stipitis NRRL Y-11545 P31867
-
-
2.7.1.17 Scheffersomyces stipitis Q9P938
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 xylitol + NAD+
-
 Scheffersomyces stipitis D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Scheffersomyces stipitis NRRL Y-11545 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Scheffersomyces stipitis NBRC 10063 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Scheffersomyces stipitis ATCC 58785 D-xylulose + NADH + H+
-
 r
1.1.1.307 xylitol + NAD(P)+
-
 Scheffersomyces stipitis D-xylose + NAD(P)H + H+
-
 r
1.1.1.307 xylitol + NAD(P)+
-
 Scheffersomyces stipitis NRRL Y-11545 D-xylose + NAD(P)H + H+
-
 r
1.1.1.307 xylitol + NAD(P)+
-
 Scheffersomyces stipitis NBRC 10063 D-xylose + NAD(P)H + H+
-
 r
1.1.1.307 xylitol + NAD(P)+
-
 Scheffersomyces stipitis ATCC 58785 D-xylose + NAD(P)H + H+
-
 r
2.7.1.17 ATP + D-xylulose
-
 Scheffersomyces stipitis ADP + D-xylulose 5-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.9 XDH
-
 Scheffersomyces stipitis
1.1.1.9 XYL2
-
 Scheffersomyces stipitis
1.1.1.9 xylitol dehydrogenase
-
 Scheffersomyces stipitis
1.1.1.307 XYL1
-
 Scheffersomyces stipitis
1.1.1.307 xylose reductase
-
 Scheffersomyces stipitis
2.7.1.17 D-xululokinase
-
 Scheffersomyces stipitis
2.7.1.17 XYL3
-
 Scheffersomyces stipitis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 NAD+
-
 Scheffersomyces stipitis
1.1.1.9 NADH
-
 Scheffersomyces stipitis
1.1.1.307 NAD+
-
 Scheffersomyces stipitis
1.1.1.307 NADH
-
 Scheffersomyces stipitis
1.1.1.307 NADP+
-
 Scheffersomyces stipitis
1.1.1.307 NADPH
-
 Scheffersomyces stipitis
2.7.1.17 ATP
-
 Scheffersomyces stipitis

General Information

EC Number General Information Comment Organism
1.1.1.9 metabolism one xylose-assimilating pathway consists of xylose reductase (XR, XYL1) and xylitol dehydrogenase (XDH, XYL2, EC 1.1.1.9) from Scheffersomyces stipitis. XR reduces xylose to xylitol by using NAD(P)H as cofactor and XDH further oxidizes xylitol to xylulose using NAD+. While the XR-XDH pathway can offer higher metabolic fluxes than the xylose isomerase (XI) pathway, it accumulates xylitol which is produced due to cofactor imbalance caused by different cofactor requirement between XR and XDH Scheffersomyces stipitis
1.1.1.307 metabolism one xylose-assimilating pathway consists of xylose reductase (XR, XYL1) and xylitol dehydrogenase (XDH, XYL2, EC 1.1.1.9) from Scheffersomyces stipitis. XR reduces xylose to xylitol by using NAD(P)H as cofactor and XDH further oxidizes xylitol to xylulose using NAD+. While the XR-XDH pathway can offer higher metabolic fluxes than the xylose isomerase (XI) pathway, it accumulates xylitol which is produced due to cofactor imbalance caused by different cofactor requirement between XR and XDH Scheffersomyces stipitis