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Literature summary extracted from

  • Yamasaki-Yashiki, S.; Komeda, H.; Hoshino, K.; Asano, Y.
    Characterization and gene cloning of L-xylulose reductase involved in L-arabinose catabolism from the pentose-fermenting fungus Rhizomucor pusillus (2017), Biosci. Biotechnol. Biochem., 81, 1612-1618 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.10 gene lxr3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Escherichia coli strain BL21(DE3) Rhizomucor pusillus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.10 2-mercaptoethanol 92% inhibition at 5 mM Rhizomucor pusillus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.10 8.71
-
 L-xylulose pH 7.0, 25°C, native enzyme Rhizomucor pusillus
1.1.1.10 13.2
-
 L-xylulose pH 7.0, 25°C, recombinant enzyme Rhizomucor pusillus
1.1.1.10 225
-
 xylitol pH 9.6, 25°C, recombinant enzyme Rhizomucor pusillus
1.1.1.10 253
-
 xylitol pH 9.6, 25°C, native enzyme Rhizomucor pusillus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.10 intracellular
-
 Rhizomucor pusillus 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.10 Mg2+ activates Rhizomucor pusillus
1.1.1.10 Mn2+ activates Rhizomucor pusillus
1.1.1.10 Zn2+ activates Rhizomucor pusillus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.10 98000
-
 gel filtration Rhizomucor pusillus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.10 xylitol + NADP+ Rhizomucor pusillus
-
 L-xylulose + NADPH + H+
-
 r
1.1.1.10 xylitol + NADP+ Rhizomucor pusillus NBRC 4578
-
 L-xylulose + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.10 Rhizomucor pusillus A0A0M4UR95
-
-
1.1.1.10 Rhizomucor pusillus NBRC 4578 A0A0M4UR95
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.10 native enzyme from cell-free extract of mycelia by dialysis, ammonium sulfate fractionation, hydrophobic interaction chromatography, again dialysis, followed by hydroxyapatite chromatography, anion exchange chromatography, ultrafiltration, and gel filtration Rhizomucor pusillus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.10 mycelium
-
 Rhizomucor pusillus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.10 53.9
-
 purified recombinant RpLXR enzyme, reduction of L-xylulose with NADPH as cofactor, pH 7.0, 25°C Rhizomucor pusillus
1.1.1.10 57.8
-
 purified recombinant RpLXR enzyme, oxidation of xylitol with NADP+ as cofactor, pH 9.6, 25°C Rhizomucor pusillus
1.1.1.10 77.3
-
 purified native RpLXR enzyme, oxidation of xylitol with NADP+ as cofactor, pH 9.6, 25°C Rhizomucor pusillus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.10 additional information substrate specificity in oxidation and reduction reactions, overview. No activity with D-mannitol, D-sorbitol, D-xylose, or D-fructose as substrates. D-Xylulose is a poor substrate. Enzyme RpLXR exhibits not only a L-xylulose reductase activity but also a strong dicarbonyl reductase activity Rhizomucor pusillus ?
-
-
1.1.1.10 additional information substrate specificity in oxidation and reduction reactions, overview. No activity with D-mannitol, D-sorbitol, D-xylose, or D-fructose as substrates. D-Xylulose is a poor substrate. Enzyme RpLXR exhibits not only a L-xylulose reductase activity but also a strong dicarbonyl reductase activity Rhizomucor pusillus NBRC 4578 ?
-
-
1.1.1.10 xylitol + NADP+
-
 Rhizomucor pusillus L-xylulose + NADPH + H+
-
 r
1.1.1.10 xylitol + NADP+
-
 Rhizomucor pusillus NBRC 4578 L-xylulose + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.10 homotetramer 4 * 29000, SDS-PAGE, 4 * 28341, sequence calculation Rhizomucor pusillus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.10 L-xylulose reductase
-
 Rhizomucor pusillus
1.1.1.10 LXR
-
 Rhizomucor pusillus
1.1.1.10 LXR3
-
 Rhizomucor pusillus
1.1.1.10 RpLXR
-
 Rhizomucor pusillus
1.1.1.10 Rplxr3
-
 Rhizomucor pusillus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.10 25
-
 assay at Rhizomucor pusillus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.10 6
-
 reduction of L-xylulose Rhizomucor pusillus
1.1.1.10 9
-
 oxidation of xylitol Rhizomucor pusillus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.10 additional information the enzyme activity toward L-xylulose with NADH is 3.1% compared to NADPH Rhizomucor pusillus
1.1.1.10 NADP+
-
 Rhizomucor pusillus
1.1.1.10 NADPH
-
 Rhizomucor pusillus

Expression

EC Number Organism Comment Expression
1.1.1.10 Rhizomucor pusillus transcription of the Rplxr3 gene in Rhizomucor pusillus strain NBRC 4578 is inhibited in the presence of D-glucose, D-xylose, and D-mannitol down
1.1.1.10 Rhizomucor pusillus transcription of the Rplxr3 gene in Rhizomucor pusillus strain NBRC 4578 is induced in the presence of L-arabinose up

General Information

EC Number General Information Comment Organism
1.1.1.10 evolution the enzyme belongs to the SDR superfamily Rhizomucor pusillus
1.1.1.10 metabolism enzyme RpLXR is involved in the L-arabinose catabolic pathway Rhizomucor pusillus