EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.41 | expressed in Escherichia coli Rosetta(DE3) cells | Xylella fastidiosa |
1.1.1.42 | expressed in Escherichia coli Rosetta(DE3) cells | Xylella fastidiosa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.41 | D268K | the coenzyme specificity of the mutant is switched from NAD+ to NADP+ | Xylella fastidiosa |
1.1.1.41 | D268K/I269Y | the coenzyme specificity of the mutant is switched from NAD+ to NADP+ | Xylella fastidiosa |
1.1.1.41 | D268K/I269Y/A275V | the coenzyme specificity of the mutant is switched from NAD+ to NADP+ | Xylella fastidiosa |
1.1.1.42 | H590L/R601L | the mutations greatly reduce the affinity for NADP+, but fail to improve the ability to use NAD+ so the mutant has similar affinities to NADP+ and NAD+ | Xylella fastidiosa |
1.1.1.42 | K589T/H590L/R601L | the mutations greatly reduce the affinity for NADP+, but fail to improve the ability to use NAD+ so the mutant has similar affinities to NADP+ and NAD+ | Xylella fastidiosa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.41 | Ca2+ | 7.9% residual activity at 2 mM | Xylella fastidiosa | |
1.1.1.41 | Co2+ | 5.2% residual activity at 2 mM | Xylella fastidiosa | |
1.1.1.41 | Cu2+ | complete inhibition at 2 mM | Xylella fastidiosa | |
1.1.1.41 | K+ | complete inhibition at 2 mM | Xylella fastidiosa | |
1.1.1.41 | Na+ | complete inhibition at 2 mM | Xylella fastidiosa | |
1.1.1.41 | Ni2+ | complete inhibition at 2 mM | Xylella fastidiosa | |
1.1.1.41 | Zn2+ | complete inhibition at 2 mM | Xylella fastidiosa | |
1.1.1.42 | Ca2+ | 3.0% residual activity at 2 mM | Xylella fastidiosa | |
1.1.1.42 | Co2+ | 8.9% residual activity at 2 mM | Xylella fastidiosa | |
1.1.1.42 | Cu2+ | complete inhibition at 2 mM | Xylella fastidiosa | |
1.1.1.42 | K+ | 3.9% residual activity at 2 mM | Xylella fastidiosa | |
1.1.1.42 | Na+ | 5.2% residual activity at 2 mM | Xylella fastidiosa | |
1.1.1.42 | Ni2+ | 3.8% residual activity at 2 mM | Xylella fastidiosa | |
1.1.1.42 | Zn2+ | complete inhibition at 2 mM | Xylella fastidiosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.41 | 0.031 | - |
NADP+ | mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 0.087 | - |
NADP+ | mutant enzyme D268K/I269Y, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 0.101 | - |
NADP+ | mutant enzyme D268K, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 0.121 | - |
NAD+ | wild type enzyme, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 0.384 | - |
NAD+ | mutant enzyme D268K, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 2.339 | - |
NADP+ | wild type enzyme, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 3.15 | - |
NAD+ | mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 4.413 | - |
NAD+ | mutant enzyme D268K/I269Y, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 0.001 | - |
NAD+ | wild type enzyme, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 0.959 | - |
NAD+ | mutant enzyme K589T/H590L/R601L, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 1.506 | - |
NAD+ | mutant enzyme H590L/R601L, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 2.455 | - |
NAD+ | wild type enzyme, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 2.695 | - |
NAD+ | mutant enzyme H590L/R601L, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 5.534 | - |
NAD+ | mutant enzyme K589T/H590L/R601L, at pH 8.0 and 25°C | Xylella fastidiosa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.41 | Mg2+ | Mg2+ is the most favored divalent cation. Mg2+ can be largely replaced by Mn2+ (69.2% activity). 2 mM is used in assay conditions | Xylella fastidiosa | |
1.1.1.42 | Mn2+ | Mn2+ is the most favored divalent cation. Mn2+ can be partly replaced by Mg2+ (17.6% activity). 2 mM is used in assay conditions | Xylella fastidiosa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 75500 | - |
MALDI-TOF mass spectrometry | Xylella fastidiosa |
1.1.1.42 | 82400 | - |
MALDI-TOF mass spectrometry | Xylella fastidiosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.41 | isocitrate + NAD+ | Xylella fastidiosa | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | Xylella fastidiosa | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.41 | Xylella fastidiosa | - |
- |
- |
1.1.1.42 | Xylella fastidiosa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.41 | metal affinity resin column chromatography | Xylella fastidiosa |
1.1.1.42 | metal affinity resin column chromatography | Xylella fastidiosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 6.3 | - |
with NADP+ as cosubstrate, at pH 8.0 and 25°C | Xylella fastidiosa |
1.1.1.41 | 96.8 | - |
with NAD+ as cosubstrate, at pH 8.0 and 25°C | Xylella fastidiosa |
1.1.1.42 | 7.4 | - |
with NAD+ as cosubstrate, at pH 8.0 and 25°C | Xylella fastidiosa |
1.1.1.42 | 73.2 | - |
with NADP+ as cosubstrate, at pH 8.0 and 25°C | Xylella fastidiosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.41 | isocitrate + NAD+ | - |
Xylella fastidiosa | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
1.1.1.41 | isocitrate + NADP+ | - |
Xylella fastidiosa | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NAD+ | - |
Xylella fastidiosa | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | - |
Xylella fastidiosa | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.41 | homodimer | 2 * 38000, SDS-PAGE | Xylella fastidiosa |
1.1.1.42 | monomer | 1 * 80000, SDS-PAGE | Xylella fastidiosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.41 | NAD+-dependent IDH | - |
Xylella fastidiosa |
1.1.1.41 | NADH-IDH | - |
Xylella fastidiosa |
1.1.1.42 | NADP+-dependent IDH | - |
Xylella fastidiosa |
1.1.1.42 | NADPH-IDH | - |
Xylella fastidiosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 55 | - |
- |
Xylella fastidiosa |
1.1.1.42 | 50 | - |
- |
Xylella fastidiosa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 40 | 47 | the enzyme is stable below 40°C and half of its activity is lost after incubation at 47°C for 20 min | Xylella fastidiosa |
1.1.1.42 | 35 | 42 | the enzyme is stable below 35°C, but its activity decreases rapidly above 35°C. The enzyme is almost inactivated after incubation at 42.5°C for 20 min | Xylella fastidiosa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.41 | 6.1 | - |
NADP+ | wild type enzyme, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 6.9 | - |
NADP+ | mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 11.8 | - |
NAD+ | mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 30.4 | - |
NADP+ | mutant enzyme D268K/I269Y, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 37.3 | - |
NAD+ | mutant enzyme D268K/I269Y, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 38.5 | - |
NADP+ | mutant enzyme D268K, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 52.8 | - |
NAD+ | mutant enzyme D268K, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 74.6 | - |
NAD+ | wild type enzyme, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 8.9 | - |
NAD+ | mutant enzyme K589T/H590L/R601L, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 16.7 | - |
NAD+ | wild type enzyme, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 30.8 | - |
NAD+ | mutant enzyme H590L/R601L, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 56.1 | - |
NAD+ | mutant enzyme K589T/H590L/R601L, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 62.4 | - |
NAD+ | mutant enzyme H590L/R601L, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 96.5 | - |
NAD+ | wild type enzyme, at pH 8.0 and 25°C | Xylella fastidiosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 8 | 9 | the enzyme retains more than 95% activity at pH 8.0-9.0 with Mg2+ | Xylella fastidiosa |
1.1.1.42 | 7.8 | - |
in the presence of Mn2+ | Xylella fastidiosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.41 | NAD+ | the enzyme displays 206fold preferences for NAD+ over NADP+ | Xylella fastidiosa | |
1.1.1.42 | NADP+ | the enzyme shows 13800fold preferences for NADP+ over NAD+ | Xylella fastidiosa |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.41 | 3 | - |
NADP+ | wild type enzyme, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 4 | - |
NAD+ | mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 8 | - |
NAD+ | mutant enzyme D268K/I269Y, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 138 | - |
NAD+ | mutant enzyme D268K, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 223 | - |
NADP+ | mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 349 | - |
NADP+ | mutant enzyme D268K/I269Y, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 381 | - |
NADP+ | mutant enzyme D268K, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.41 | 617 | - |
NAD+ | wild type enzyme, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 7 | - |
NAD+ | wild type enzyme, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 9 | - |
NAD+ | mutant enzyme K589T/H590L/R601L, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 10 | - |
NAD+ | mutant enzyme K589T/H590L/R601L, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 20 | - |
NAD+ | mutant enzyme H590L/R601L, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 23 | - |
NAD+ | mutant enzyme H590L/R601L, at pH 8.0 and 25°C | Xylella fastidiosa | |
1.1.1.42 | 96500 | - |
NAD+ | wild type enzyme, at pH 8.0 and 25°C | Xylella fastidiosa |