Any feedback?
Literature summary extracted from
- Structure and allosteric regulation of human NAD-dependent isocitrate dehydrogenase (2020), Cell Discov., 6, 94 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.41 | ADP | - |
Homo sapiens |  |
| 1.1.1.41 | citrate | - |
Homo sapiens | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.41 | expressed in Escherichia coli BL21(DE3) Codon-Plus cells | Homo sapiens |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.1.1.41 | isoform IDH3 containing a beta mutant in apo form, hanging drop vapor diffusion method, using 0.05M NH4Cl, 0.05 Bis-Tris (pH 6.5), and 30% (w/v) pentaerythritol ethoxylate | Homo sapiens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.41 | mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.41 | Mn2+ | 2 mM used in assay conditions | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.41 | isocitrate + NAD+ | Homo sapiens | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.41 | Homo sapiens | P50213 and O43837 and P51553 | subunits alpha, beta and gamma | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.41 | Ni-NTA column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.41 | isocitrate + NAD+ | - |
Homo sapiens | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.41 | heterooctamer | - |
Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.41 | IDH3 | - |
Homo sapiens |
| 1.1.1.41 | NAD-dependent isocitrate dehydrogenase | - |
Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.41 | NAD+ | - |
Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
