EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.42 | expressed in Escherichia coli BL21 Gold (DE3) cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.42 | A134D | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
1.1.1.42 | H133Q | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
1.1.1.42 | R100Q | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
1.1.1.42 | R132A | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
1.1.1.42 | R132C | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
1.1.1.42 | R132G | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
1.1.1.42 | R132H | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
1.1.1.42 | R132K | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
1.1.1.42 | R132N | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
1.1.1.42 | R132Q | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
1.1.1.42 | R132W | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.42 | 0.0025 | - |
NADPH | Km less than 0.0025 mM or equal, mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.005 | - |
NADPH | Km less than 0.005 mM or equal, mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.005 | - |
NADPH | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.01 | - |
NADPH | Km less than 0.01 mM or equal, wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.01 | - |
NADPH | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.015 | - |
isocitrate | wild type enzyme, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.025 | - |
NADPH | Km less than 0.025 mM or equal, mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.025 | - |
NADPH | Km less than 0.025 mM or equal, mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.025 | - |
NADPH | Km less than 0.025 mM or equal, mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.025 | - |
NADPH | Km less than 0.025 mM, mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.03 | - |
NADP+ | wild type enzyme, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.067 | - |
NADP+ | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.07 | - |
NADP+ | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.08 | - |
NADP+ | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.101 | - |
NADP+ | mutant enzyme H133Q, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.11 | - |
2-oxoglutarate | mutant enzyme R132A, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.14 | - |
NADP+ | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.16 | - |
NADP+ | mutant enzyme H133Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.18 | - |
NADP+ | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.22 | - |
isocitrate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.26 | - |
2-oxoglutarate | mutant enzyme R132Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.28 | - |
isocitrate | mutant enzyme H133Q, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.3 | - |
2-oxoglutarate | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.34 | - |
2-oxoglutarate | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.36 | - |
2-oxoglutarate | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.36 | - |
2-oxoglutarate | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.4 | - |
isocitrate | mutant enzyme H133Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.5 | - |
2-oxoglutarate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.58 | - |
NADP+ | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.61 | - |
2-oxoglutarate | mutant enzyme R132K, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.7 | - |
NADP+ | mutant enzyme A134D, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.75 | - |
NADP+ | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.8 | - |
isocitrate | mutant enzyme R132Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.82 | - |
2-oxoglutarate | mutant enzyme R132W, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 1 | - |
NADP+ | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 1.1 | - |
2-oxoglutarate | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 1.1 | - |
isocitrate | mutant enzyme R132K, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 1.2 | - |
NADP+ | mutant enzyme A134D, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 1.5 | - |
isocitrate | mutant enzyme R132N, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 1.6 | - |
NADP+ | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 1.8 | - |
2-oxoglutarate | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 2.7 | - |
isocitrate | mutant enzyme A134D, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 3.6 | - |
isocitrate | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 3.6 | - |
isocitrate | mutant enzyme R132W, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 4.2 | - |
isocitrate | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 5.3 | - |
isocitrate | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 5.7 | - |
isocitrate | mutant enzyme R132A, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 6 | - |
isocitrate | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 7 | - |
isocitrate | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 8 | - |
isocitrate | mutant enzyme A134D, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 8 | - |
isocitrate | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 8.2 | - |
isocitrate | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 9 | - |
isocitrate | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 10 | - |
2-oxoglutarate | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 10 | - |
2-oxoglutarate | mutant enzyme R132N, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 12 | - |
2-oxoglutarate | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.42 | cytosol | - |
Homo sapiens | 5829 | - |
1.1.1.42 | peroxisome | - |
Homo sapiens | 5777 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.42 | Mg2+ | 10 mM used in assay conditions | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.42 | isocitrate + NADP+ | Homo sapiens | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.42 | Homo sapiens | O75874 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.42 | Ni-NTA column chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.42 | 2-oxoglutarate + NADPH + H+ | the reaction is catalyzed by diverse R132 mutant enzymes | Homo sapiens | D-2-hydroxyglutarate + NADP+ | - |
r | |
1.1.1.42 | isocitrate + NADP+ | - |
Homo sapiens | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.42 | IDH1 | - |
Homo sapiens |
1.1.1.42 | isocitrate dehydrogenase 1 | - |
Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 49.1 | - |
melting temperature | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.42 | 0.016 | - |
2-oxoglutarate | kcat less than 0.016 s-1 or equal, mutant enzyme H133Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.017 | - |
2-oxoglutarate | wild type enzyme, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.019 | - |
2-oxoglutarate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.019 | - |
2-oxoglutarate | kcat less than 0.019 s-1 or equal, mutant enzyme A134D, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.02 | - |
2-oxoglutarate | mutant enzyme A134D, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.034 | - |
2-oxoglutarate | mutant enzyme H133, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.047 | - |
isocitrate | mutant enzyme R132N, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.12 | - |
isocitrate | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.128 | - |
2-oxoglutarate | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.2 | - |
isocitrate | mutant enzyme A134D, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.34 | - |
2-oxoglutarate | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.37 | - |
2-oxoglutarate | mutant enzyme R132A, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.43 | - |
2-oxoglutarate | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.45 | - |
2-oxoglutarate | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.54 | - |
2-oxoglutarate | mutant enzyme R132W, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.57 | - |
2-oxoglutarate | mutant enzyme R132K, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.79 | - |
2-oxoglutarate | mutant enzyme R132N, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.84 | - |
2-oxoglutarate | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 1 | - |
isocitrate | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 1.21 | - |
isocitrate | mutant enzyme R132W, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 1.4 | - |
isocitrate | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 1.59 | - |
2-oxoglutarate | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 1.6 | - |
2-oxoglutarate | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 1.61 | - |
isocitrate | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 2.3 | - |
isocitrate | mutant enzyme A134D, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 2.4 | - |
isocitrate | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 4.2 | - |
2-oxoglutarate | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 4.4 | - |
isocitrate | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 4.7 | - |
2-oxoglutarate | mutant enzyme R132Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 5.6 | - |
isocitrate | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 7.2 | - |
isocitrate | mutant enzyme R132K, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 9.2 | - |
isocitrate | mutant enzyme R132Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 9.3 | - |
isocitrate | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 9.4 | - |
isocitrate | mutant enzyme H133Q, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 10.4 | - |
isocitrate | mutant enzyme R132A, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 11 | - |
isocitrate | wild type enzyme, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 45 | - |
isocitrate | mutant enzyme H133Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 85 | - |
isocitrate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.42 | NADP+ | - |
Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.42 | 0.013 | - |
2-oxoglutarate | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.02 | - |
isocitrate | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.028 | - |
2-oxoglutarate | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.031 | - |
isocitrate | mutant enzyme R132N, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.04 | - |
2-oxoglutarate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.074 | - |
isocitrate | mutant enzyme A134D, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.08 | - |
2-oxoglutarate | mutant enzyme R132N, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.16 | - |
isocitrate | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.24 | - |
2-oxoglutarate | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.28 | - |
isocitrate | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.29 | - |
isocitrate | mutant enzyme A134D, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.3 | - |
isocitrate | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 0.34 | - |
isocitrate | mutant enzyme R132W, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.54 | - |
isocitrate | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.57 | - |
isocitrate | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.659 | - |
2-oxoglutarate | mutant enzyme R132W, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.7 | - |
isocitrate | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 0.9 | - |
2-oxoglutarate | mutant enzyme R132K, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 1.3 | - |
isocitrate | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 1.5 | - |
2-oxoglutarate | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 1.8 | - |
isocitrate | mutant enzyme R132A, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 2.3 | - |
2-oxoglutarate | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 3.4 | - |
2-oxoglutarate | mutant enzyme R132A, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 3.8 | - |
2-oxoglutarate | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 4.4 | - |
2-oxoglutarate | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 5 | - |
2-oxoglutarate | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 7 | - |
isocitrate | mutant enzyme R132K, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 12 | - |
isocitrate | mutant enzyme R132Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 18 | - |
2-oxoglutarate | mutant enzyme R132Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 35 | - |
isocitrate | mutant enzyme H133Q, at pH 7.5 and 21°C | Homo sapiens | |
1.1.1.42 | 110 | - |
isocitrate | mutant enzyme H133Q, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 390 | - |
isocitrate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
1.1.1.42 | 730 | - |
isocitrate | wild type enzyme, at pH 7.5 and 21°C | Homo sapiens |