Literature summary extracted from
Cho, H.J.; Cho, H.Y.; Park, J.W.; Kwon, O.S.; Lee, H.S.; Huh, T.L.; Kang, B.S.
NADP+-dependent cytosolic isocitrate dehydrogenase provides NADPH in the presence of cadmium due to the moderate chelating effect of glutathione (2018), J. Biol. Inorg. Chem., 23, 849-860 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.1.1.42 |
expressed in Escherichia coli BL21(DE3) cells |
Mus musculus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.1.1.42 |
hanging drop vapor diffusion method, using 0.1 M sodium citrate, pH 5.5, 16% (w/v) PEG 4000, 20% (v/v) isopropanol |
Mus musculus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.1.1.42 |
C245S |
the activities of the mutant in the presence of Cd2+ are decreased as that of wild type enzyme |
Mus musculus |
1.1.1.42 |
C379S |
the activity of the mutant in the absence of Cd2+ is similar to that of the wild type enzyme, but the decrease of activity in the presence of Cd2+ is much reduced |
Mus musculus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.1.1.42 |
Cd2+ |
above 0.1 mM, the enzyme loses activity, and at 2 mM Cd2+ most of the activity has disappeared. Chelation of Cd2+ by dithiothreitol cannot recover the lost enzyme activity. Inactivation of the enzyme by Cd2+ is less effective when the enzyme is activated with Cd2+ than Mg2+, More than 50% of the activity of the enzyme activated with 0.05 mM Cd2+ remains in the presence of 1 mM GSH |
Mus musculus |
|
1.1.1.42 |
Mn2+ |
the specific activity of purified enzyme is increased by Mn2+ in the micromolar range |
Mus musculus |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.1.1.42 |
cytosol |
- |
Mus musculus |
5829 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.1.1.42 |
Cd2+ |
the enzyme activity is increased at a Cd2+ concentration below 0.1 mM |
Mus musculus |
|
1.1.1.42 |
Mg2+ |
the maximum activity with Mg2+ is slightly lower than that with Cd2+ |
Mus musculus |
|
1.1.1.42 |
additional information |
Ca2+ does not activate Mg2+ |
Mus musculus |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.1.1.42 |
isocitrate + NADP+ |
Mus musculus |
- |
2-oxoglutarate + CO2 + NADPH + H+ |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.1.1.42 |
Mus musculus |
O88844 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.1.1.42 |
glutathione Sepharose column chromatography |
Mus musculus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.1.1.42 |
isocitrate + NADP+ |
- |
Mus musculus |
2-oxoglutarate + CO2 + NADPH + H+ |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.1.1.42 |
IDH |
- |
Mus musculus |
1.1.1.42 |
NADP+-dependent isocitrate dehydrogenase |
- |
Mus musculus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.1.1.42 |
NADP+ |
- |
Mus musculus |
|