EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.27 | I229A | 30% decrease in Km value and 2.6fold increase in kcat value for phenylpyruvate | Lacticaseibacillus casei |
1.1.1.27 | Q88R | 17% decrease in Km value and 3fold increase in kcat value for phenylpyruvate | Lacticaseibacillus casei |
1.1.1.27 | T235G | slight decrease in specific activity towards phenylpyruvate | Lacticaseibacillus casei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.27 | 1.86 | - |
phenylpyruvate | mutant Q88R, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 2.5 | 5 | phenylpyruvate | mutant I229A, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 3.5 | - |
phenylpyruvate | wild-type, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 5.75 | - |
phenylpyruvate | mutant I229A, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 6.82 | - |
phenylpyruvate | mutant Q88R, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 8.23 | - |
phenylpyruvate | wild-type, pH 5.5, 30°C | Lacticaseibacillus casei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.27 | Lacticaseibacillus casei | A0A2U9AUU1 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 15.5 | - |
wild-type, substrate benzoylformic acid, pH 5.5, 30°C | Lacticaseibacillus casei |
1.1.1.27 | 17.4 | - |
wild-type, substrate 4-hydroxyphenylpyruvate, pH 5.5, 30°C | Lacticaseibacillus casei |
1.1.1.27 | 25 | 40 | wild-type, substrate pyruvate, pH 5.5, 30°C | Lacticaseibacillus casei |
1.1.1.27 | 66.7 | - |
mutant T235G, substrate phenylpyruvate, pH 5.5, 30°C | Lacticaseibacillus casei |
1.1.1.27 | 72.3 | - |
wild-type, substrate phenylpyruvate, pH 5.5, 30°C | Lacticaseibacillus casei |
1.1.1.27 | 168.4 | - |
wild-type, substrate 2-oxobutanoate, pH 5.5, 30°C | Lacticaseibacillus casei |
1.1.1.27 | 435.8 | - |
mutant I229A, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei |
1.1.1.27 | 451.5 | - |
mutant Q88R, substrate phenylpyruvate, pH 5.5, 30°C | Lacticaseibacillus casei |
1.1.1.27 | 512.4 | - |
mutant I229A, substrate phenylpyruvate, pH 5.5, 30°C | Lacticaseibacillus casei |
1.1.1.27 | 671.2 | - |
wild-type, substrate oxaloacetic acid, pH 5.5, 30°C | Lacticaseibacillus casei |
1.1.1.27 | 680.3 | - |
mutant T235G, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei |
1.1.1.27 | 798 | - |
wild-type, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei |
1.1.1.27 | 4071 | - |
mutant Q88R, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.27 | 2-oxobutanoate + NADH + H+ | - |
Lacticaseibacillus casei | 2-hydroxybutanoate + NAD+ | - |
? | |
1.1.1.27 | 4-hydroxyphenylpyruvate + NADH + H+ | - |
Lacticaseibacillus casei | L-4-hydroxyphenyllactate + NAD+ | - |
? | |
1.1.1.27 | benzoylformic acid + NADH + H+ | - |
Lacticaseibacillus casei | ? + NAD+ | - |
? | |
1.1.1.27 | oxaloacetate + NADH + H+ | - |
Lacticaseibacillus casei | ? + NAD+ | - |
? | |
1.1.1.27 | phenylpyruvate + NADH + H+ | - |
Lacticaseibacillus casei | L-phenyllactate + NAD+ | - |
? | |
1.1.1.27 | pyruvate + NADH + H+ | - |
Lacticaseibacillus casei | L-lactate + NAD+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.27 | ? | x * 36800, SDS-PAGE | Lacticaseibacillus casei |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.27 | 94.7 | - |
phenylpyruvate | wild-type, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 344.4 | - |
phenylpyruvate | mutant I229A, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 380.4 | - |
phenylpyruvate | mutant Q88R, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 1293 | - |
phenylpyruvate | mutant I229A, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 6467 | - |
phenylpyruvate | wild-type, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 17479 | - |
phenylpyruvate | mutant Q88R, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.27 | 11.5 | - |
phenylpyruvate | wild-type, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 55.8 | - |
phenylpyruvate | mutant Q88R, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 59.9 | - |
phenylpyruvate | mutant I229A, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 507 | - |
phenylpyruvate | mutant I229A, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 1848 | - |
phenylpyruvate | wild-type, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
1.1.1.27 | 9397 | - |
phenylpyruvate | mutant Q88R, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei |