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Literature summary extracted from

  • Yuan, X.; Wang, J.; Lin, J.; Yang, L.; Wu, M.
    Efficient production of xylitol by the integration of multiple copies of xylose reductase gene and the deletion of Embden-Meyerhof-Parnas pathway-associated genes to enhance NADPH regeneration in Escherichia coli (2019), J. Ind. Microbiol. Biotechnol., 46, 1061-1069 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.10 synthesis potential approach for industrial-scale production of xylitol from hemicellulosic hydrolysate involving the enzyme Neurospora crassa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.10 gene xr, recombinant expression in Escherichia coli strain W3110 from p43-XR comprising a constitutive promotor and gene xr, subcloning in Escherichia coli strain DH5alpha, gene xr expression analysis by quantitative RT-PCR Neurospora crassa

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.10 additional information Escherichia coli strain WZ04 is first constructed by a simultaneous deletion-insertion strategy involving CRISPR/Cas9 markerless gene-editing technology, replacing ptsG, xylAB and ptsF in wild-type Escherichia coli strain W3110 with three mutated xylose reductase genes (xr) from Neurospora crassa. In a second approach, the pfkA, pfkB, pgi and/or sthA genes are deleted and replaced by xr to investigate the influence of carbon flux toward the pentose phosphate pathway and/or transhydrogenase activity on NADPH generation. The deletion of pfkA/pfkB significantly improves NADPH supply, but minimally influences cell growth. The effects of insertion position and copy number of xr are examined by a quantitative real-time PCR and a shake-flask fermentation experiment. In a fed-batch fermentation experiment with a 15-l bioreactor, strain WZ51 produces 131.6 g/l xylitol from hemicellulosic hydrolysate (xylitol productivity: 2.09 g/l/h). The biotransformation process involves resting cell catalysis with pure xylose as the substrate, but not hemicellulosic hydrolysate. Cofactor ratios are measured for single gene replacement strains and strains with combinatorial gene replacements, all based on strain WZ04, overview Neurospora crassa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.10 cytosol
-
 Neurospora crassa 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.10 xylitol + NADP+ Neurospora crassa
-
 L-xylulose + NADPH + H+
-
 r
1.1.1.10 xylitol + NADP+ Neurospora crassa CBS 708.71
-
 L-xylulose + NADPH + H+
-
 r
1.1.1.10 xylitol + NADP+ Neurospora crassa 74-OR23-1A
-
 L-xylulose + NADPH + H+
-
 r
1.1.1.10 xylitol + NADP+ Neurospora crassa DSM 1257
-
 L-xylulose + NADPH + H+
-
 r
1.1.1.10 xylitol + NADP+ Neurospora crassa ATCC 24698
-
 L-xylulose + NADPH + H+
-
 r
1.1.1.10 xylitol + NADP+ Neurospora crassa FGSC 987
-
 L-xylulose + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.10 Neurospora crassa Q7SD67
-
-
1.1.1.10 Neurospora crassa 74-OR23-1A Q7SD67
-
-
1.1.1.10 Neurospora crassa ATCC 24698 Q7SD67
-
-
1.1.1.10 Neurospora crassa CBS 708.71 Q7SD67
-
-
1.1.1.10 Neurospora crassa DSM 1257 Q7SD67
-
-
1.1.1.10 Neurospora crassa FGSC 987 Q7SD67
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.10 xylitol + NADP+
-
 Neurospora crassa L-xylulose + NADPH + H+
-
 r
1.1.1.10 xylitol + NADP+
-
 Neurospora crassa CBS 708.71 L-xylulose + NADPH + H+
-
 r
1.1.1.10 xylitol + NADP+
-
 Neurospora crassa 74-OR23-1A L-xylulose + NADPH + H+
-
 r
1.1.1.10 xylitol + NADP+
-
 Neurospora crassa DSM 1257 L-xylulose + NADPH + H+
-
 r
1.1.1.10 xylitol + NADP+
-
 Neurospora crassa ATCC 24698 L-xylulose + NADPH + H+
-
 r
1.1.1.10 xylitol + NADP+
-
 Neurospora crassa FGSC 987 L-xylulose + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.10 XylB
-
 Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.10 30
-
 in vivo assay at Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.10 NADP+
-
 Neurospora crassa
1.1.1.10 NADPH
-
 Neurospora crassa

General Information

EC Number General Information Comment Organism
1.1.1.10 physiological function cofactor supply is a rate-limiting step in the bioconversion of xylose to xylitol Neurospora crassa