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Literature summary extracted from

  • Philipp, D.P.; Parsons, P.
    Kinetic characterization of long chain fatty acyl coenzyme A ligase from rat liver mitochondria (1979), J. Biol. Chem., 254, 10785-10790.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.3 AMP
-
 Rattus norvegicus
6.2.1.3 ATP above 15 mM Rattus norvegicus
6.2.1.3 Brij 58 maximal inhibition at 4% detergent Rattus norvegicus
6.2.1.3 diphosphate
-
 Rattus norvegicus
6.2.1.3 sodium cholate maximal inhibition at 1% detergent Rattus norvegicus
6.2.1.3 Triton X-100 maximal inhibition at 4% detergent Rattus norvegicus
6.2.1.3 Tween 80 maximal inhibition at 4% detergent Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.3 0.05
-
 ATP CoA Rattus norvegicus
6.2.1.3 4.1
-
 ATP at 20 mM MgCl2 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.3 mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.3 Mg2+ required Rattus norvegicus
6.2.1.3 Mg2+ maximal activity at 15-20 mM MgCl2, no inhibition at higher concentrations Rattus norvegicus
6.2.1.3 Mn2+ divalent cation required, Mn2+ can replace Mg2+ in activation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.3 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.2.1.3 ATP + a long-chain fatty acid + CoA = AMP + diphosphate + a long-chain fatty acyl-CoA mitochondrial long chain fatty acyl-CoA ligase, unlike short chain ligases and medium chain ligases, does not utilize an adenylate as an intermediate in the formation of fatty acyl-CoA Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.3 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.3 ATP + palmitate + CoA
-
 Rattus norvegicus AMP + diphosphate + palmitoyl-CoA
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.3 7.5 8.3
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.3 6 9.8 pH 6: about 50% of maximal activity, pH 8.9: about 60% of maximal activity Rattus norvegicus