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Literature summary extracted from

  • Xu, Y.; Liu, L.; Nakamura, A.; Someya, S.; Miyakawa, T.; Tanokura, M.
    Studies on the regulatory mechanism of isocitrate dehydrogenase 2 using acetylation mimics (2017), Sci. Rep., 7, 9785 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.42 SIRT3 under calorie restriction, mitochondrial deacetylase Sirt3 deacetylates and activates isoform IDH2 Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 expressed in Escherichia coli KRX cells Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.42 wild type and mutant enzyme K256Q, sitting drop vapor diffusion method, using 0.1 M sodium citrate tribasic dehydrate (pH 5.6), 11.5% (v/v) 2-propanol and 10.8% (w/v) PEG 4000 Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.42 K106Q the activity of the mutant is about 118% of the wild type value Mus musculus
1.1.1.42 K155Q the activity of the mutant is about 110% of the wild type value Mus musculus
1.1.1.42 K166Q the activity of the mutant is about 80% of the wild type value Mus musculus
1.1.1.42 K180Q the activity of the mutant is about 60% of the wild type value Mus musculus
1.1.1.42 K243Q the activity of the mutant is about 90% of the wild type value Mus musculus
1.1.1.42 K251Q inactive Mus musculus
1.1.1.42 K256Q the activity of the mutant is about 40% of the wild type value Mus musculus
1.1.1.42 K263Q the activity of the mutant is about 80% of the wild type value Mus musculus
1.1.1.42 K272Q the activity of the mutant is about 65% of the wild type value Mus musculus
1.1.1.42 K275Q the activity of the mutant is about 85% of the wild type value Mus musculus
1.1.1.42 K282Q the activity of the mutant is about 105% of the wild type value Mus musculus
1.1.1.42 K360Q the mutant shows wild type activity Mus musculus
1.1.1.42 K384Q the activity of the mutant is about 90% of the wild type value Mus musculus
1.1.1.42 K413Q the activity of the mutant is about 20% of the wild type value Mus musculus
1.1.1.42 K442Q the activity of the mutant is about 90% of the wild type value Mus musculus
1.1.1.42 K80Q the activity of the mutant is about 105% of the wild type value Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.0148
-
 NADP+ mutant K256Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 0.0304
-
 isocitrate mutant K256Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 0.0337
-
 NADP+ wild type isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 0.0544
-
 isocitrate wild type isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 0.0555
-
 isocitrate mutant K413Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 0.1122
-
 NADP+ mutant K413Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 mitochondrion
-
 Mus musculus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+ 1 mM used in assay conditions Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Mus musculus
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Mus musculus P54071
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 Ni-NTA resin column chromatography and Resource S column chromatography Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Mus musculus 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 IDH2
-
 Mus musculus
1.1.1.42 isocitrate dehydrogenase 2
-
 Mus musculus
1.1.1.42 NADP+-dependent IDH
-
 Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.42 2.66
-
 isocitrate mutant K256Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 3.13
-
 NADP+ mutant K256Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 4.61
-
 isocitrate mutant K413Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 5.6
-
 isocitrate wild type isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 6.36
-
 NADP+ wild type isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 7.69
-
 NADP+ mutant K413Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.42 59
-
 NADP+ mutant K413Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 83
-
 isocitrate mutant K413Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 88
-
 isocitrate mutant K256Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 111
-
 isocitrate wild type isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 186
-
 NADP+ wild type isoform IDH2, at pH 7.4 and 25°C Mus musculus
1.1.1.42 205
-
 NADP+ mutant K256Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus