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Literature summary extracted from

  • Wun, S.J.; Johnson, L.A.; You, L.; McGeary, R.P.; Brueck, T.; Schenk, G.; Guddat, L.W.
    Inhibition studies of ketol-acid reductoisomerases from pathogenic microorganisms (2020), Arch. Biochem. Biophys., 692, 108516 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.86 expression in Escherichia coli BL21 Campylobacter jejuni
1.1.1.86 expression in Escherichia coli BL21 Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.86 2-dimethylphosphoryl-2-hydroxy-acetic acid i.e. Hoe 704, reversible inhibitor Campylobacter jejuni
1.1.1.86 2-dimethylphosphoryl-2-hydroxy-acetic acid i.e. Hoe 704, reversible inhibitor Mycobacterium tuberculosis
1.1.1.86 cyclopropane-1,1-dicarboxylate reversible inhibitor Campylobacter jejuni
1.1.1.86 cyclopropane-1,1-dicarboxylate reversible inhibitor Mycobacterium tuberculosis
1.1.1.86 N-Hydroxy-N-isopropyloxamate irreversible Campylobacter jejuni
1.1.1.86 N-Hydroxy-N-isopropyloxamate
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.86 0.29
-
 Hydroxypyruvate pH 8.0, 25°C Mycobacterium tuberculosis
1.1.1.86 0.58
-
 Hydroxypyruvate pH 8.0, 25°C Campylobacter jejuni
1.1.1.86 2.4
-
 2-acetolactate pH 8.0, 25°C Campylobacter jejuni
1.1.1.86 6
-
 (2S)-2-acetolactate pH 8.0, 25°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Campylobacter jejuni
-
-
-
1.1.1.86 Mycobacterium tuberculosis P9WKJ7
-
-
1.1.1.86 Mycobacterium tuberculosis ATCC 25618 P9WKJ7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.86
-
 Campylobacter jejuni
1.1.1.86
-
 Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.86 0.4
-
 substrate: hydroxypyruvate, pH 8.0, 25°C Mycobacterium tuberculosis
1.1.1.86 0.5
-
 substrate: hydroxypyruvate, pH 8.0, 25°C Campylobacter jejuni
1.1.1.86 1.4
-
 substrate: 2-acetolactate, pH 8.0, 25°C Campylobacter jejuni
1.1.1.86 6
-
 substrate: (2S)-2-acetolactate, pH 8.0, 25°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 (2S)-2-acetolactate + NADPH + H+
-
 Mycobacterium tuberculosis 3-hydroxy-3-methyl-2-oxobutyrate + NADP+
-
 ?
1.1.1.86 (2S)-2-acetolactate + NADPH + H+
-
 Mycobacterium tuberculosis ATCC 25618 3-hydroxy-3-methyl-2-oxobutyrate + NADP+
-
 ?
1.1.1.86 2-acetolactate + NADPH
-
 Campylobacter jejuni 3-hydroxy-3-methyl-2-oxobutyrate + NADP+
-
 ?
1.1.1.86 hydroxypyruvate + NADPH + H+
-
 Campylobacter jejuni glycerate + NADP+
-
 ?
1.1.1.86 hydroxypyruvate + NADPH + H+
-
 Mycobacterium tuberculosis glycerate + NADP+
-
 ?
1.1.1.86 hydroxypyruvate + NADPH + H+
-
 Mycobacterium tuberculosis ATCC 25618 glycerate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.86 dimer
-
 Mycobacterium tuberculosis
1.1.1.86 dodecamer
-
 Campylobacter jejuni

Synonyms

EC Number Synonyms Comment Organism
1.1.1.86 KARI
-
 Campylobacter jejuni
1.1.1.86 KARI
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.86 0.2
-
 Hydroxypyruvate pH 8.0, 25°C Mycobacterium tuberculosis
1.1.1.86 0.3
-
 Hydroxypyruvate pH 8.0, 25°C Campylobacter jejuni
1.1.1.86 0.7
-
 2-acetolactate pH 8.0, 25°C Campylobacter jejuni
1.1.1.86 3.7
-
 (2S)-2-acetolactate pH 8.0, 25°C Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADPH
-
 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.86 0.000026
-
 N-Hydroxy-N-isopropyloxamate pH 8.0, 25°C Campylobacter jejuni
1.1.1.86 0.000026
-
 N-Hydroxy-N-isopropyloxamate pH 8.0, 25°C Mycobacterium tuberculosis
1.1.1.86 0.0003
-
 2-dimethylphosphoryl-2-hydroxy-acetic acid pH 8.0, 25°C Campylobacter jejuni
1.1.1.86 0.0003
-
 2-dimethylphosphoryl-2-hydroxy-acetic acid pH 8.0, 25°C Mycobacterium tuberculosis
1.1.1.86 0.00303
-
 cyclopropane-1,1-dicarboxylate pH 8.0, 25°C Campylobacter jejuni
1.1.1.86 0.00303
-
 cyclopropane-1,1-dicarboxylate pH 8.0, 25°C Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
1.1.1.86 drug target target for the discovery of biocides Campylobacter jejuni
1.1.1.86 drug target target for the discovery of biocides Mycobacterium tuberculosis
1.1.1.86 metabolism the enzyme is involved in the branched-chain amino acid biosynthesis Campylobacter jejuni
1.1.1.86 metabolism the enzyme is involved in the branched-chain amino acid biosynthesis Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.86 0.55
-
 Hydroxypyruvate pH 8.0, 25°C Campylobacter jejuni
1.1.1.86 0.63
-
 (2S)-2-acetolactate pH 8.0, 25°C Mycobacterium tuberculosis
1.1.1.86 0.77
-
 Hydroxypyruvate pH 8.0, 25°C Mycobacterium tuberculosis
1.1.1.86 27
-
 2-acetolactate pH 8.0, 25°C Campylobacter jejuni