EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.2.8 | a plasmid construct is used to express PQQ-DH9. DH9. The expression host is a derivative Gluconobacter sp. strain of CHM43, which lacks the genes for PQQ-dependent glycerol dehydrogenase and the membrane-bound alcohol dehydrogenase and consequently has minimal ability to oxidize primary and secondary alcohols. The membranes of the transformant exhibited considerable d-arabitol dehydrogenase activity, whereas the reference strain did not, even if it had PQQ-DH9-encoding genes in the chromosome and harbored the empty vector. This suggests that PQQ-DH9 is not expressed in the genome | Gluconobacter sp. CHM43 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.2.8 | Gluconobacter sp. CHM43 | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.2.8 | 0.0031 | - |
substrate: threitol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.0052 | - |
substrate: D-arabinose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.01 | - |
substrate: trans-1,2-cyclohexanediol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.018 | - |
substrate: glycerol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.022 | - |
substrate: 1-cyclohexylethanol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.027 | - |
substrate: D-mannitol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.028 | - |
substrate: ribitol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.03 | - |
substrate: 2-hexanol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.035 | - |
substrate: isopropanol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.055 | - |
substrate: D-sorbitol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.065 | - |
substrate: 1,3-butanediol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.08 | - |
substrate: 1,2-butanediol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.084 | - |
substrate: cyclohexanol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.085 | - |
substrate: 2,4-pentanediol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.09 | - |
substrate: D-ribose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.1 | - |
substrate: D-lyxose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.12 | - |
substrate: D-xylose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.12 | - |
substrate: L-arabinose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.13 | - |
substrate: D-galactose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.13 | - |
substrate: D-gluconate, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.14 | - |
substrate: 2-butanol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.14 | - |
substrate: glyceraldehyde, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.17 | - |
substrate: D-arabitol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.19 | - |
substrate: meso-erythritol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.2 | - |
substrate: L-ribose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.35 | - |
substrate: 1,2-cyclopentandiol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.4 | - |
substrate: 2,3-butanediol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
1.1.2.8 | 0.55 | - |
substrate: cis-1,2-cyclohexanediol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.8 | 1,2-butanediol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | 1,2-cyclopentandiol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | 1,3-butanediol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | 1-cyclohexylethanol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | 2,3-butanediol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | 2,4-pentanediol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | 2-butanol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | 2-hexanol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | cis-1,2-cyclohexanediol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | cyclohexanol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | D-arabinose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | D-arabitol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | D-galactose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | D-gluconate + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | D-lyxose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | D-mannitol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | D-ribose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | D-sorbitol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | D-xylose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | glyceraldehyde + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | glycerol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | isopropanol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | L-arabinose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | L-ribose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | meso-erythritol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | additional information | the enzyme oxidizes a wide variety of secondary alcohols but has higher KM-values than PQQ-dependent glycerol dehydrogenase with regard to linear substrates such as glycerol. Cyclic substrates such as cis-1,2-cyclohexanediol are readily oxidized | Gluconobacter sp. CHM43 | ? | - |
- |
|
1.1.2.8 | ribitol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | threitol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1.1.2.8 | trans-1,2-cyclohexanediol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.2.8 | PQQ-DH9 | - |
Gluconobacter sp. CHM43 |
1.1.2.8 | pyrroloquinoline quinone-dependent dehydrogenase | - |
Gluconobacter sp. CHM43 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.8 | pyrroloquinoline quinone | dependent on | Gluconobacter sp. CHM43 |