Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lee, S.; Jeon, H.; Giri, P.; Lee, U.; Jung, H.; Lim, S.; Sarak, S.; Khobragade, T.; Kim, B.; Yun, H.
    The reductive amination of carbonyl compounds using native amine dehydrogenase from Laribacter hongkongensis (2021), Biotechnol. Bioprocess Eng., 26, 384-391 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.4.1.28 synthesis reductive amination of cyclohexanone (up to 100 mM) into cyclohexylamine is performed with an AmDH and formate dehydrogenase system with more than 99% conversion using Escherichia coli whole cell as well as purified enzymes. Chiral amine are produced from the corresponding ketone using inexpensive ammonium formate as sole sacrificial agent and enzymes omega-transaminase, AmDH, and formate dehydrogenase Laribacter hongkongensis
1.4.1.28 synthesis reductive amination of cyclohexanone (up to 100 mM) into cyclohexylamine using transgenic Escherichia coli as whole-cell reactors as well as purified enzymes Laribacter hongkongensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.28 expression in Escherichia coli Laribacter hongkongensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.28 homology modeling of structure. The coordination of the amine group to E108 holds the tetrahedral carbon of the substrate bearing the amine Laribacter hongkongensis

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.28 Laribacter hongkongensis
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.28 170
-
 substrate cyclohexanone, pH not specified in the publication, temperature not specified in the publication Laribacter hongkongensis
1.4.1.28 364
-
 substrate isovaleraldehyde, pH not specified in the publication, temperature not specified in the publication Laribacter hongkongensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.28 3-methylbutanal + NH3 + NADH + H+ 214% of the activity with cyclohexanone Laribacter hongkongensis 3-methylbutan-1-amine + H2O + NAD+
-
 ?
1.4.1.28 acetophenone + NH3 + NADH + H+
-
 Laribacter hongkongensis (S)-alpha-methylbenzylamine + H2O + NAD+
-
 ?
1.4.1.28 benzaldehyde + NH3 + NAD(P)H + H+
-
 Laribacter hongkongensis benzylamine + H2O + NAD(P)+
-
 ?
1.4.1.28 benzaldehyde + NH3 + NADH + H+ 10.4% of the activity with cyclohexanone Laribacter hongkongensis benzylamine + H2O + NAD+
-
 ?
1.4.1.28 butan-2-one + NH3 + NADH + H+ 5.5% of the activity with cyclohexanone Laribacter hongkongensis 2-butylamine + H2O + NAD+
-
 ?
1.4.1.28 cyclohexanone + NH3 + NAD(P)H + H+
-
 Laribacter hongkongensis cyclohexylamine + H2O + NAD(P)+
-
 ?
1.4.1.28 cyclohexanone + NH3 + NADH + H+
-
 Laribacter hongkongensis cyclohexylamine + H2O + NAD+
-
 ?
1.4.1.28 cyclopentanone + NH3 + NADH + H+ 3.4% of the activity with cyclohexanone Laribacter hongkongensis cyclopentylamine + H2O + NAD+
-
 ?
1.4.1.28 isovaleraldehyde + NH3 + NAD(P)H + H+
-
 Laribacter hongkongensis 3-methylbutan-1-amine + H2O + NAD(P)+
-
 ?
1.4.1.28 additional information poor substrates: linear aliphatic carbonyl compounds such as 3-heptanone, 2-pentanone as well as cyclopentanone Laribacter hongkongensis ?
-
-

Subunits

EC Number Subunits Comment Organism
1.4.1.28 ? x * 36800, SDS-PAGE, recombinant protein Laribacter hongkongensis

Synonyms

EC Number Synonyms Comment Organism
1.4.1.28 AMDH
-
 Laribacter hongkongensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.28 9
-
-
 Laribacter hongkongensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.28 NADH
-
 Laribacter hongkongensis

General Information

EC Number General Information Comment Organism
1.4.1.28 metabolism in the docking analysis, cyclohexanone is well-orientated with -5.4 kcal/mol of binding energy and 3.16 A distance from the side chain of E104, key residue for interacting ammonia and substrate Laribacter hongkongensis