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Literature summary extracted from

  • Khan, R.; Zeb, A.; Roy, N.; Thapa Magar, R.; Kim, H.J.; Lee, K.W.; Lee, S.W.
    Biochemical and structural basis of triclosan resistance in a novel enoyl-acyl carrier protein reductase (2018), Antimicrob. Agents Chemother., 62, e00648-18 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.104 expressed in Escherichia coli mutant JPP1111 uncultured bacterium pBF1

General Stability

EC Number General Stability Organism
1.3.1.104 no enzymatic activity is observed in 100 mM sodium citrate buffer regardless of the pH uncultured bacterium pBF1

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.104 additional information the enzyme is resistant to triclosan uncultured bacterium pBF1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.104 0.009627
-
 crotonyl-CoA at pH 7.0 and 25°C uncultured bacterium pBF1
1.3.1.104 0.02764
-
 NADPH at pH 7.0 and 25°C uncultured bacterium pBF1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.104 crotonyl-[acyl-carrier-protein] + NADPH + H+ uncultured bacterium pBF1
-
 butyryl-[acyl-carrier-protein] + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.104 uncultured bacterium pBF1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.104 crotonyl-CoA + NADPH + H+
-
 uncultured bacterium pBF1 butyryl-CoA + NADP+
-
 ?
1.3.1.104 crotonyl-[acyl-carrier-protein] + NADPH + H+
-
 uncultured bacterium pBF1 butyryl-[acyl-carrier-protein] + NADP+
-
 ?
1.3.1.104 additional information the enzyme shows no 7-alpha hydroxysteroid dehydrogenase activity when tested with cholic acid substrate and NADH and NADPH cofactors. The purified protein does not utilize NADH as a cofactor when tested with crotonyl-[CoA] as substrate uncultured bacterium pBF1 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.3.1.104 ? x * 33560, calculated from amino acid sequence uncultured bacterium pBF1

Synonyms

EC Number Synonyms Comment Organism
1.3.1.104 enoyl-ACP reductase
-
 uncultured bacterium pBF1
1.3.1.104 enoyl-acyl carrier protein reductase
-
 uncultured bacterium pBF1
1.3.1.104 ENR
-
 uncultured bacterium pBF1
1.3.1.104 Fabl2
-
 uncultured bacterium pBF1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.104 0.009627
-
 crotonyl-CoA at pH 7.0 and 25°C uncultured bacterium pBF1
1.3.1.104 0.02764
-
 NADPH at pH 7.0 and 25°C uncultured bacterium pBF1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.104 7
-
 in 100 mM sodium phosphate buffer uncultured bacterium pBF1

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.104 FAD
-
 uncultured bacterium pBF1
1.3.1.104 FMN
-
 uncultured bacterium pBF1
1.3.1.104 NADPH
-
 uncultured bacterium pBF1