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Literature summary extracted from

  • Yang, T.; Shao, Y.H.; Guo, L.Z.; Meng, X.L.; Yu, H.; Lu, W.D.
    Role of N,N-dimethylglycine and its catabolism to sarcosine in Chromohalobacter salexigens DSM 3043 (2020), Appl. Environ. Microbiol., 86, e01186 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.8.4 expression in Escherichia coli Chromohalobacter salexigens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.8.4 EDTA 2 mM, 33% inhibition Chromohalobacter salexigens
1.5.8.4 additional information Na+, K+, Ca2+, Zn2+, Mn2+, Mg2+, Fe2+, and Co2+ display no or slightly inhibitory effects at 2 mM Chromohalobacter salexigens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.8.4 8.6
-
 N,N-dimethylglycine pH 7.5, 60°C Chromohalobacter salexigens
1.5.8.4 14.65
-
 sarcosine pH 7.5, 60°C Chromohalobacter salexigens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.8.4 Ca2+ 2 mM, 12% increase in activity Chromohalobacter salexigens
1.5.8.4 additional information Na+, K+, Ca2+, Zn2+, Mn2+, Mg2+, Fe2+, and Co2+ display no or slightly inhibitory effects at 2 mM Chromohalobacter salexigens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.8.4 79000
-
 gel filtration Chromohalobacter salexigens

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.4 Chromohalobacter salexigens Q1QYW1
-
-
1.5.8.4 Chromohalobacter salexigens DSM 3043 Q1QYW1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.4 N,N-dimethylglycine + electron-transfer flavoprotein + H2O
-
 Chromohalobacter salexigens sarcosine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
-
 r
1.5.8.4 N,N-dimethylglycine + electron-transfer flavoprotein + H2O
-
 Chromohalobacter salexigens DSM 3043 sarcosine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
-
 r
1.5.8.4 sarcosine + electron-transfer flavoprotein + H2O
-
 Chromohalobacter salexigens glycine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
-
 r
1.5.8.4 sarcosine + electron-transfer flavoprotein + H2O
-
 Chromohalobacter salexigens DSM 3043 glycine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.8.4 monomer 1 * 77200, SDS-PAGE, 1 * 78507, calculated from sequence Chromohalobacter salexigens

Synonyms

EC Number Synonyms Comment Organism
1.5.8.4 Csal_0990
-
 Chromohalobacter salexigens
1.5.8.4 DdhC
-
 Chromohalobacter salexigens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.8.4 60
-
-
 Chromohalobacter salexigens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.5.8.4 70
-
 64% of maximum activity Chromohalobacter salexigens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.8.4 0.28
-
 sarcosine pH 7.5, 60°C Chromohalobacter salexigens
1.5.8.4 0.32
-
 N,N-dimethylglycine pH 7.5, 60°C Chromohalobacter salexigens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.8.4 7
-
-
 Chromohalobacter salexigens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.8.4 FAD noncovalently bound flavin adenine dinucleotide, purified protein shows the absorption spectrum of an oxidized flavoprotein, with maxima at 222, 275, 370, and 440 nm and one shoulder at around 470 nm Chromohalobacter salexigens
1.5.8.4 NAD+ Km value 0.8 mM, NAD+ is preferred over NADP+ Chromohalobacter salexigens
1.5.8.4 NADP+ Km value 3.2 mM, NAD+ is preferred over NADP+ Chromohalobacter salexigens