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Literature summary extracted from

  • Yang, T.; Shao, Y.; Guo, L.; Meng, X.; Yu, H.; Lu, W.
    Role of N, N-dimethylglycine and its catabolism to sarcosine in Chromohalobacter salexigens DSM 3043 (2020), Appl. Environ. Microbiol., 86, e01186-20 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.7.3 expression in Escherichia coli Chromohalobacter salexigens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.7.3 EDTA 2 mM, 33% inhibition Chromohalobacter salexigens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.7.3 8.61
-
 N,N-dimethylglycine pH 7.5, 60°C Chromohalobacter salexigens
1.5.7.3 14.85
-
 sarcosine pH 7.5, 60°C Chromohalobacter salexigens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.7.3 Ca2+ 2 mM, 12% increase in activity Chromohalobacter salexigens
1.5.7.3 additional information neither inhibitory nor activating: Na+, K+, Zn2+, Mn2+, Mg2+, Fe2+, Co2+ Chromohalobacter salexigens

Organism

EC Number Organism UniProt Comment Textmining
1.5.7.3 Chromohalobacter salexigens Q1QYW1
-
-
1.5.7.3 Chromohalobacter salexigens DSM 3043 Q1QYW1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.7.3 recombinant protein, using Ni2x02-chelating affinity chromatography Chromohalobacter salexigens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.7.3 N,N-dimethylglycine + 2 oxidized ferredoxin + H2O
-
 Chromohalobacter salexigens sarcosine + formaldehyde + 2 reduced ferredoxin + 2 H+
-
 ?
1.5.7.3 N,N-dimethylglycine + 2 oxidized ferredoxin + H2O
-
 Chromohalobacter salexigens DSM 3043 sarcosine + formaldehyde + 2 reduced ferredoxin + 2 H+
-
 ?
1.5.7.3 sarcosine + 2 oxidized ferredoxin + H2O
-
 Chromohalobacter salexigens glycine + formaldehyde + 2 reduced ferredoxin + 2 H+
-
 ?
1.5.7.3 sarcosine + 2 oxidized ferredoxin + H2O
-
 Chromohalobacter salexigens DSM 3043 glycine + formaldehyde + 2 reduced ferredoxin + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.7.3 monomer 1 * 79000, SDS-PAGE Chromohalobacter salexigens

Synonyms

EC Number Synonyms Comment Organism
1.5.7.3 Csal_0990
-
 Chromohalobacter salexigens
1.5.7.3 DdhC
-
 Chromohalobacter salexigens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.7.3 60
-
-
 Chromohalobacter salexigens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.5.7.3 70
-
 64% of maximum activity Chromohalobacter salexigens
1.5.7.3 80
-
 24% of maximum activity Chromohalobacter salexigens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.7.3 50
-
 60 min, 36% residual activity Chromohalobacter salexigens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.7.3 0.28
-
 sarcosine pH 7.5, 60°C Chromohalobacter salexigens
1.5.7.3 0.31
-
 N,N-dimethylglycine pH 7.5, 60°C Chromohalobacter salexigens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.7.3 7 8
-
 Chromohalobacter salexigens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.7.3 4 11
-
 Chromohalobacter salexigens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.7.3 FAD noncovalently bound Chromohalobacter salexigens
1.5.7.3 NAD+ prefers NAD+ to NADP+, Km and kcat values are 0.81 mM and 0.20 per sec, respectively Chromohalobacter salexigens
1.5.7.3 NADP+ prefers NAD+ to NADP+, Km and kcat values are 3.21 mM and 0.14 per sec, respectively Chromohalobacter salexigens

General Information

EC Number General Information Comment Organism
1.5.7.3 physiological function under high-salinity conditions, the presence of dimethylglycine inhibits growth of the wild type and induces the production and accumulation of trehalose and glucosylglycerate intracellularly. Exogenous addition of dimethylglycine significantly improves the growth rates of DdhC mutants incubated at 37°C synthetic medium with sarcosine as the sole N source Chromohalobacter salexigens