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Literature summary extracted from

  • Lahham, M.; Jha, S.; Goj, D.; Macheroux, P.; Wallner, S.
    The family of sarcosine oxidases Same reaction, different products (2021), Arch. Biochem. Biophys., 704, 108868 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.1 K171A the mutant shows 39% activity of the wild type enzyme Corynebacterium sp. U-96
1.5.3.1 K171D the mutant shows 32% activity of the wild type enzyme Corynebacterium sp. U-96
1.5.3.1 K171R the mutant shows 58% activity of the wild type enzyme Corynebacterium sp. U-96
1.5.3.1 K358A inactive Corynebacterium sp. U-96
1.5.3.1 K358D inactive Corynebacterium sp. U-96
1.5.3.1 Y358R the variant displays 0.07% activity and a higher apparent KM for sarcosine as compared to wild type enzyme Corynebacterium sp. U-96

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.1 sarcosine + 5,6,7,8-tetrahydrofolate + O2 Corynebacterium sp. U-96
-
 glycine + formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.1 Corynebacterium sp. U-96 Q50LF0 and Q50LF2 and Q50LE9 and Q50LF1 subunits alpha, beta, gamma, and delta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.1 sarcosine + 5,6,7,8-tetrahydrofolate + O2
-
 Corynebacterium sp. U-96 glycine + formaldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.1 heterotetramer 1 * 103000 + 1 * 44000 + 1 * 21000 + 1 * 11000, SDS-PAGE Corynebacterium sp. U-96

Synonyms

EC Number Synonyms Comment Organism
1.5.3.1 heterotetrameric sarcosine oxidase
-
 Corynebacterium sp. U-96
1.5.3.1 TSOX
-
 Corynebacterium sp. U-96

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.1 FAD
-
 Corynebacterium sp. U-96
1.5.3.1 FMN
-
 Corynebacterium sp. U-96
1.5.3.1 NAD+ NAD+ is not involved in sarcosine oxidation or in redox equilibrium with the flavins. However, it has a critical effect on the folding and/or stability of the alpha subunit Corynebacterium sp. U-96