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Literature summary extracted from

  • Song, Q.; Wang, B.; Zhao, F.; Han, Y.; Zhou, Z.
    Expression, characterization and molecular docking of the assimilatory NADH-nitrite reductase from Acidovorax wautersii QZ-4 (2020), Biochem. Eng. J., 159, 107589 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.15 expressed in Escherichia coli BL21(DE3) cells Acidovorax wautersii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.15 Al3+ 87.62% residual activity at 5 mM Acidovorax wautersii
1.7.1.15 Ca2+ 43.78% residual activity at 5 mM Acidovorax wautersii
1.7.1.15 Cu2+ 65.3% residual activity at 5 mM Acidovorax wautersii
1.7.1.15 cyanide 20.75% residual activity at 1 mM Acidovorax wautersii
1.7.1.15 additional information dithiothreitol and EDTA have no evident effects on enzyme activity Acidovorax wautersii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.15 Fe3+ 131.97% activity at 5 mM Acidovorax wautersii
1.7.1.15 additional information Mg2+ and Mn2+ have no evident effects on enzyme activity Acidovorax wautersii
1.7.1.15 Zn2+ 126.21% activity at 5 mM Acidovorax wautersii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.15 nitrite + 3 NADH + 5 H+ Acidovorax wautersii
-
 NH3 + 3 NAD+ + 2 H2O
-
 ?
1.7.1.15 nitrite + 3 NADH + 5 H+ Acidovorax wautersii QZ-4
-
 NH3 + 3 NAD+ + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.15 Acidovorax wautersii
-
-
-
1.7.1.15 Acidovorax wautersii QZ-4
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.15 nitrite + 3 NADH + 5 H+
-
 Acidovorax wautersii NH3 + 3 NAD+ + 2 H2O
-
 ?
1.7.1.15 nitrite + 3 NADH + 5 H+
-
 Acidovorax wautersii QZ-4 NH3 + 3 NAD+ + 2 H2O
-
 ?
1.7.1.15 nitrite + reduced methyl viologen methyl viologen is the best in vitro electron donor Acidovorax wautersii NH3 + oxidized methyl viologen
-
 ?
1.7.1.15 nitrite + reduced methyl viologen methyl viologen is the best in vitro electron donor Acidovorax wautersii QZ-4 NH3 + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.15 heterodimer 1 * 100000 + 1 * 14000, SDS-PAGE Acidovorax wautersii
1.7.1.15 heterodimer 1 * 91750 + 1 * 12400, calculated from amino acid sequence Acidovorax wautersii

Synonyms

EC Number Synonyms Comment Organism
1.7.1.15 assimilatory NADH-nitrite reductase
-
 Acidovorax wautersii
1.7.1.15 NirBD
-
 Acidovorax wautersii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.15 30
-
 over 93% activity is retained at 40°C. At 20 and 50°C, the enzyme retains more than 73 and 66% activity, respectively Acidovorax wautersii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.15 6
-
-
 Acidovorax wautersii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.1.15 5 7 the enzyme exhibits 95 % of its maximum activity at pH 6.5. At pH 5.0 and 7.0, it remains over 80% relative activity Acidovorax wautersii

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.15 NADH
-
 Acidovorax wautersii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.7.1.15 Acidovorax wautersii subunit nirD, calculated from amino acid sequence
-
 5.7
1.7.1.15 Acidovorax wautersii subunit nirB, calculated from amino acid sequence
-
 6.3