EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.3.24 | expressed in Escherichia coli XL-1 Blue cells | Corynebacterium sp. P-1 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.3.24 | H175A | the mutant contains 1 mol of covalently bound flavin (FMN) and exhibits catalytic activity similar to the wild type enzyme | Corynebacterium sp. P-1 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.3.24 | sarcosine + 5,6,7,8-tetrahydrofolate + O2 | Corynebacterium sp. P-1 | - |
glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.3.24 | Corynebacterium sp. P-1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.3.24 | - |
Corynebacterium sp. P-1 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.3.24 | 8.4 | - |
mutant enzyme H175A, pH and temperature not specified in the publication | Corynebacterium sp. P-1 |
1.5.3.24 | 8.6 | - |
wild type enzyme, pH and temperature not specified in the publication | Corynebacterium sp. P-1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.3.24 | sarcosine + 5,6,7,8-tetrahydrofolate + O2 | - |
Corynebacterium sp. P-1 | glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.3.24 | heterotetramer | - |
Corynebacterium sp. P-1 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.3.24 | sarcosine oxidase | - |
Corynebacterium sp. P-1 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.3.24 | FAD | the wild type enzyme contains 1 mol of noncovalently bound FAD | Corynebacterium sp. P-1 | |
1.5.3.24 | FMN | the wild type enzyme contains 1 mol of covalently bound FMN attached to subunit beta | Corynebacterium sp. P-1 | |
1.5.3.24 | NAD+ | the wild type enzyme contains 1 mol of noncovalently bound NAD+ | Corynebacterium sp. P-1 |