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Literature summary extracted from

  • Kalms, J.; Banthiya, S.; Galemou Yoga, E.; Hamberg, M.; Holzhutter, H.G.; Kuhn, H.; Scheerer, P.
    The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity (2017), Biochim. Biophys. Acta, 1862, 463-473 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.12 expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.12 mutant enzyme A420G, sitting drop vapor diffusion method, using 12% (w/v) polyethylene glycol 3350, 0.2 M MgCl2 and 0.1 M tris(hydroxymethyl)-aminomethane at pH 7.1 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.12 A420G the mutation leads to an enzyme variant with pronounced dual specificity favoring arachidonic acid 11R-oxygenation. The mutation impairs the catalytic activity by about 80% and leads to 2fold improved oxygen affinity compared to the wild type Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.12 Pseudomonas aeruginosa Q8RNT4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.12 Ni-NTA agarose column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.12 arachidonate + O2
-
Pseudomonas aeruginosa (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
?
1.13.11.12 linoleate + O2
-
Pseudomonas aeruginosa (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.12 ? x * 70000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.13.11.12 arachidonic acid 15S-lipoxygenating enzyme
-
Pseudomonas aeruginosa
1.13.11.12 LOX
-
Pseudomonas aeruginosa