EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.12 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.13.11.12 | mutant enzyme A420G, sitting drop vapor diffusion method, using 12% (w/v) polyethylene glycol 3350, 0.2 M MgCl2 and 0.1 M tris(hydroxymethyl)-aminomethane at pH 7.1 | Pseudomonas aeruginosa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.11.12 | A420G | the mutation leads to an enzyme variant with pronounced dual specificity favoring arachidonic acid 11R-oxygenation. The mutation impairs the catalytic activity by about 80% and leads to 2fold improved oxygen affinity compared to the wild type | Pseudomonas aeruginosa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.12 | Pseudomonas aeruginosa | Q8RNT4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.12 | Ni-NTA agarose column chromatography | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.12 | arachidonate + O2 | - |
Pseudomonas aeruginosa | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? | |
1.13.11.12 | linoleate + O2 | - |
Pseudomonas aeruginosa | (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.12 | ? | x * 70000, SDS-PAGE | Pseudomonas aeruginosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.12 | arachidonic acid 15S-lipoxygenating enzyme | - |
Pseudomonas aeruginosa |
1.13.11.12 | LOX | - |
Pseudomonas aeruginosa |