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Literature summary extracted from

  • Williams, E.; Sharrock, A.; Rylott, E.; Bruce, N.; MacKichan, J.; Ackerley, D.
    A cofactor consumption screen identifies promising NfsB family nitroreductases for dinitrotoluene remediation (2019), Biotechnol. Lett., 41, 1155-1162 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.B2 expressed in Escherichia coli BL21(DE3) cells Vibrio vulnificus
1.7.1.B2 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.B2 2,4-dinitrotoluene
-
 Bacillus subtilis
1.7.1.B2 2,4-dinitrotoluene
-
 Vibrio vulnificus
1.7.1.B2 2,6-dinitrotoluene
-
 Bacillus subtilis
1.7.1.B2 2,6-dinitrotoluene
-
 Vibrio vulnificus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.B2 0.062
-
 2,4-dinitrotoluene at pH 8.0 and 30°C Bacillus subtilis
1.7.1.B2 0.32
-
 2,4-dinitrotoluene at pH 8.0 and 30°C Vibrio vulnificus
1.7.1.B2 0.51
-
 2,6-dinitrotoluene at pH 8.0 and 30°C Bacillus subtilis
1.7.1.B2 1.6
-
 2,6-dinitrotoluene at pH 8.0 and 30°C Vibrio vulnificus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.B2 2,4-dinitrotoluene + NADPH + H+ Vibrio vulnificus
-
 ?
-
 ?
1.7.1.B2 2,4-dinitrotoluene + NADPH + H+ Bacillus subtilis highest activity ?
-
 ?
1.7.1.B2 2,4-dinitrotoluene + NADPH + H+ Vibrio vulnificus ATCC 27562
-
 ?
-
 ?
1.7.1.B2 2,4-dinitrotoluene + NADPH + H+ Bacillus subtilis 168 highest activity ?
-
 ?
1.7.1.B2 2,6-dinitrotoluene + NADPH + H+ Bacillus subtilis
-
 ?
-
 ?
1.7.1.B2 2,6-dinitrotoluene + NADPH + H+ Vibrio vulnificus highest activity ?
-
 ?
1.7.1.B2 2,6-dinitrotoluene + NADPH + H+ Vibrio vulnificus ATCC 27562 highest activity ?
-
 ?
1.7.1.B2 2,6-dinitrotoluene + NADPH + H+ Bacillus subtilis 168
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.B2 Bacillus subtilis O34475
-
-
1.7.1.B2 Bacillus subtilis 168 O34475
-
-
1.7.1.B2 Vibrio vulnificus Q7MCD2
-
-
1.7.1.B2 Vibrio vulnificus ATCC 27562 Q7MCD2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.B2 Ni-NTA resin column chromatography Vibrio vulnificus
1.7.1.B2 Ni-NTA resin column chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.B2 2,4-dinitrotoluene + NADPH + H+
-
 Vibrio vulnificus ?
-
 ?
1.7.1.B2 2,4-dinitrotoluene + NADPH + H+ highest activity Bacillus subtilis ?
-
 ?
1.7.1.B2 2,4-dinitrotoluene + NADPH + H+
-
 Vibrio vulnificus ATCC 27562 ?
-
 ?
1.7.1.B2 2,4-dinitrotoluene + NADPH + H+ highest activity Bacillus subtilis 168 ?
-
 ?
1.7.1.B2 2,6-dinitrotoluene + NADPH + H+
-
 Bacillus subtilis ?
-
 ?
1.7.1.B2 2,6-dinitrotoluene + NADPH + H+ highest activity Vibrio vulnificus ?
-
 ?
1.7.1.B2 2,6-dinitrotoluene + NADPH + H+ highest activity Vibrio vulnificus ATCC 27562 ?
-
 ?
1.7.1.B2 2,6-dinitrotoluene + NADPH + H+
-
 Bacillus subtilis 168 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.B2 NfsB
-
 Vibrio vulnificus
1.7.1.B2 YfkO
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.1.B2 22
-
 2,6-dinitrotoluene at pH 8.0 and 30°C Bacillus subtilis
1.7.1.B2 90
-
 2,6-dinitrotoluene at pH 8.0 and 30°C Vibrio vulnificus
1.7.1.B2 140
-
 2,4-dinitrotoluene at pH 8.0 and 30°C Bacillus subtilis
1.7.1.B2 200
-
 2,4-dinitrotoluene at pH 8.0 and 30°C Vibrio vulnificus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.7.1.B2 0.33
-
 at pH 8.0 and 30°C Bacillus subtilis 2,4-dinitrotoluene
1.7.1.B2 0.51
-
 at pH 8.0 and 30°C Vibrio vulnificus 2,4-dinitrotoluene
1.7.1.B2 0.67
-
 at pH 8.0 and 30°C Bacillus subtilis 2,6-dinitrotoluene
1.7.1.B2 0.8
-
 at pH 8.0 and 30°C Vibrio vulnificus 2,6-dinitrotoluene

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7.1.B2 43
-
 2,6-dinitrotoluene at pH 8.0 and 30°C Bacillus subtilis
1.7.1.B2 57
-
 2,6-dinitrotoluene at pH 8.0 and 30°C Vibrio vulnificus
1.7.1.B2 640
-
 2,4-dinitrotoluene at pH 8.0 and 30°C Vibrio vulnificus