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Literature summary extracted from

  • Chu, S.; Zhang, D.; Wang, D.; Zhi, Y.; Zhou, P.
    Heterologous expression and biochemical characterization of assimilatory nitrate and nitrite reductase reveals adaption and potential of Bacillus megaterium NCT-2 in secondary salinization soil (2017), Int. J. Biol. Macromol., 101, 1019-1028 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.3 expression in Escherichia coli Priestia megaterium
1.7.1.15 expression in Escherichia coli Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.3 Cu2+ 1 mM, 76% of initial activity Priestia megaterium
1.7.1.3 additional information not inhibitory nor activating: Ba2+, Zn2+, EDTA Priestia megaterium
1.7.1.15 Al3+ 1 mM, 84% of initial activity Priestia megaterium
1.7.1.15 Ca2+ 1 mM, 80% of initial activity Priestia megaterium
1.7.1.15 Cu2+ 1 mM, 54% initial activity Priestia megaterium
1.7.1.15 Mg2+ 1 mM, 82% of initial activity Priestia megaterium
1.7.1.15 additional information not inhibitory nor activating: Ba2+, EDTA Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.3 0.67
-
 nitrate pH 7.1, 30°C Priestia megaterium
1.7.1.15 3.1
-
 nitrite pH 7.1, 30°C Priestia megaterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.3 Al3+ 1 mM, 113% of initial activity Priestia megaterium
1.7.1.3 Ca2+ 1 mM, 136% of initial activity Priestia megaterium
1.7.1.3 Fe2+ 1 mM, 137% of initial activity Priestia megaterium
1.7.1.3 Mg2+ 1 mM, 120% of initial activity Priestia megaterium
1.7.1.3 Mn2+ 1 mM, 114% of initial activity Priestia megaterium
1.7.1.3 additional information not inhibitory nor activating: Ba2+, Zn2+, EDTA Priestia megaterium
1.7.1.15 Fe2+ 1 mM, 226% of initial activity Priestia megaterium
1.7.1.15 Mn2+ 1 mM, 110% of initial activity Priestia megaterium
1.7.1.15 additional information not inhibitory nor activating: Ba2+, EDTA Priestia megaterium
1.7.1.15 Zn2+ 1 mM, 113% of initial activity Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.3 Priestia megaterium A0A8D3X5C3 and A0A8D3X5J3 A0A8D3X5C3 i.e catalytic subunit NasC, A0A8D3X5J3 i.e. electron transfer subunit NasD
-
1.7.1.3 Priestia megaterium WSH-002 A0A8D3X5C3 and A0A8D3X5J3 A0A8D3X5C3 i.e catalytic subunit NasC, A0A8D3X5J3 i.e. electron transfer subunit NasD
-
1.7.1.15 Priestia megaterium A0A8D4BLJ9 and A0A8D4BRF4 A0A8D4BLJ9 i.e subunit NasE, A0A8D4BRF4 i.e. electron transfer subunit NasB
-
1.7.1.15 Priestia megaterium WSH-002 A0A8D4BLJ9 and A0A8D4BRF4 A0A8D4BLJ9 i.e subunit NasE, A0A8D4BRF4 i.e. electron transfer subunit NasB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.3 NAD(P)H + H+ + nitrate
-
 Priestia megaterium NAD(P)+ + nitrite + H2O
-
 ?
1.7.1.3 NAD(P)H + H+ + nitrate
-
 Priestia megaterium WSH-002 NAD(P)+ + nitrite + H2O
-
 ?
1.7.1.3 reduced methyl viologen + nitrate the optimum electron donor is methyl viologen plus Na2S2O4 Priestia megaterium oxidized methyl viologen + nitrite + H2O
-
 ?
1.7.1.3 reduced methyl viologen + nitrate the optimum electron donor is methyl viologen plus Na2S2O4 Priestia megaterium WSH-002 oxidized methyl viologen + nitrite + H2O
-
 ?
1.7.1.15 nitrite + NADH + H+
-
 Priestia megaterium nitrite + NAD+ + H2O
-
 ?
1.7.1.15 nitrite + NADH + H+
-
 Priestia megaterium WSH-002 nitrite + NAD+ + H2O
-
 ?
1.7.1.15 nitrite + reduced methyl viologen the optimum electron donor is methyl viologen plus Na2S2O4 Priestia megaterium ammonia + oxidized methyl viologen
-
 ?
1.7.1.15 nitrite + reduced methyl viologen the optimum electron donor is methyl viologen plus Na2S2O4 Priestia megaterium WSH-002 ammonia + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.3 ? x * 87300, electron transfer subunit, x * 80500 catalytic subunit, SDS-PAGE Priestia megaterium
1.7.1.15 ? x * 87336, electron transfer subunit, x * 80100 catalytic subunit, SDS-PAGE Priestia megaterium

Synonyms

EC Number Synonyms Comment Organism
1.7.1.15 BMWSH_4089
-
 Priestia megaterium
1.7.1.15 NasB
-
 Priestia megaterium
1.7.1.15 nasE
-
 Priestia megaterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.3 35
-
-
 Priestia megaterium
1.7.1.15 30
-
-
 Priestia megaterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.7.1.3 40
-
 more than 90% of maximum activity Priestia megaterium
1.7.1.15 35
-
 95% of maximum activity Priestia megaterium
1.7.1.15 80
-
 50% of maximum activity Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.3 6.2
-
-
 Priestia megaterium
1.7.1.15 6.5
-
-
 Priestia megaterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.1.3 6.5
-
 more than 99% of maximum activity Priestia megaterium
1.7.1.15 6.8
-
 95% of maximum activity Priestia megaterium