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Literature summary extracted from

  • Nidetzky, B.; Brueggler, K.; Kratzer, R.; Mayr, P.
    Multiple forms of xylose reductase in Candida intermedia comparison of their functional properties using quantitative structure-activity relationships, steady-state kinetic analysis, and pH studies (2003), J. Agric. Food Chem., 51, 7930-7935 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.430 synthesis dual specific xylose reductase (dsXR) has an about 4fold higher specificity for NADH than NADPH. This fact could make this enzyme an interesting candidate to be used in metabolic engineering of the yeast xylose metabolism, likely in Saccharomyces cerevisiae. Increased levels of dsXR activity could contribute to an improvement of ethanol production from D-xylose by reducing the cofactor imbalance of the initial catabolic pathway [Candida] intermedia
1.1.1.431 synthesis dual specific xylose reductase (dsXR) has an about 4fold higher specificity for NADH than NADPH. This fact could make this enzyme an interesting candidate to be used in metabolic engineering of the yeast xylose metabolism, likely in Saccharomyces cerevisiae. Increased levels of dsXR activity could contribute to an improvement of ethanol production from D-xylose by reducing the cofactor imbalance of the initial catabolic pathway [Candida] intermedia

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.307 NAD+
-
 [Candida] intermedia
1.1.1.307 NADH
-
 [Candida] intermedia
1.1.1.307 NADP+
-
 [Candida] intermedia
1.1.1.307 NADPH
-
 [Candida] intermedia
1.1.1.430 NAD+ for dual specific xylose reductase (dsXR) NAD+ behaves as a competitive inhibitor against NADH. Competitive inhibition of is observed both at unsaturating and saturating concentrations of xylose [Candida] intermedia
1.1.1.430 NADH for dual specific xylose reductase (dsXR) NADH behaves as a competitive inhibitor against NAD+. Competitive inhibition of is observed both at unsaturating and saturating concentrations of xylitol [Candida] intermedia
1.1.1.430 NADP+ for monospecific xylose reductase (msXR) and dual specific xylose reductase (dsXR) NADP+ behaves as a competitive inhibitor against NADPH. Competitive inhibition of is observed both at unsaturating and saturating concentrations of xylose [Candida] intermedia
1.1.1.430 NADPH for monospecific xylose reductase (msXR) and dual specific xylose reductase (dsXR) NADPH behaves as a competitive inhibitor against NADP+. Competitive inhibition of is observed both at unsaturating and saturating concentrations of xylitol [Candida] intermedia
1.1.1.431 NADP+ competitive inhibitor against NADPH; for monospecific xylose reductase (msXR) and dual specific xylose reductase (dsXR) NADP+ behaves as a competitive inhibitor against NADPH. Competitive inhibition of is observed both at unsaturating and saturating concentrations of xylose [Candida] intermedia
1.1.1.431 NADPH competitive inhibitor against NADP+; for monospecific xylose reductase (msXR) and dual specific xylose reductase (dsXR) NADPH behaves as a competitive inhibitor against NADP+. Competitive inhibition of is observed both at unsaturating and saturating concentrations of xylitol [Candida] intermedia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.307 0.31
-
 NADP+ pH 7, 25°C [Candida] intermedia
1.1.1.307 0.37
-
 NAD+ pH 7, 25°C [Candida] intermedia
1.1.1.307 0.8
-
 xylitol cosubstrate NADP+, pH 7, 25°C [Candida] intermedia
1.1.1.307 0.9
-
 xylitol cosubstrate NAD+, pH 7, 25°C [Candida] intermedia
1.1.1.307 19
-
 NADH pH 7, 25°C [Candida] intermedia
1.1.1.307 30
-
 NADPH pH 7, 25°C [Candida] intermedia
1.1.1.307 30
-
 D-xylose cosubstrate NADH, pH 7, 25°C [Candida] intermedia
1.1.1.307 64
-
 D-xylose cosubstrate NADPH, pH 7, 25°C [Candida] intermedia
1.1.1.430 0.007
-
 D-fucose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 0.01
-
 D-xylose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 0.015
-
 D-galactose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 0.02
-
 D-erythrose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 0.028
-
 L-arabinose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 0.033
-
 D-glucose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 0.068
-
 D-ribose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 0.126
-
 2-deoxy-D-galactose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 0.144
-
 L-Lyxose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 2.43
-
 DL-glyceraldehyde pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 13.2
-
 propionaldehyde pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 14.7
-
 pentanal pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 33
-
 Butanal pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.431 0.006
-
 D-glucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 0.01
-
 D-fucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 0.016
-
 NADP+ pH 7, 25°C [Candida] intermedia
1.1.1.431 0.016
-
 D-xylose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 0.02
-
 L-arabinose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 0.028
-
 NADPH pH 7, 25°C [Candida] intermedia
1.1.1.431 0.033
-
 D-erythrose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 0.038
-
 2-deoxy-D-ribose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 0.058
-
 2-deoxy-D-glucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 0.061
-
 D-galactose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 0.091
-
 D-ribose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 0.117
-
 L-Lyxose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 0.221
-
 2-deoxy-D-galactose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 1.14
-
 DL-glyceraldehyde pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 2 3 Butanal pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 3.9
-
 pentanal pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 77
-
 D-xylose pH 7, 25°C [Candida] intermedia
1.1.1.431 78
-
 propionaldehyde pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 480
-
 xylitol pH 7, 25°C [Candida] intermedia

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.307 [Candida] intermedia
-
-
-
1.1.1.430 [Candida] intermedia
-
-
-
1.1.1.431 [Candida] intermedia
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.307 D-xylose + NADH + H+
-
 [Candida] intermedia xylitol + NAD+
-
 r
1.1.1.307 D-xylose + NADPH + H+
-
 [Candida] intermedia xylitol + NADP+
-
 r
1.1.1.307 xylitol + NAD+
-
 [Candida] intermedia D-xylose + NADH + H+
-
 r
1.1.1.307 xylitol + NADP+
-
 [Candida] intermedia D-xylose + NADPH + H+
-
 r
1.1.1.430 2-deoxy-D-galactose + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia ?
-
 ?
1.1.1.430 2-deoxy-D-ribose + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia ?
-
 ?
1.1.1.430 butanal + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia ?
-
 ?
1.1.1.430 D-erythrose + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia ?
-
 ?
1.1.1.430 D-fucose + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia ?
-
 ?
1.1.1.430 D-galactose + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia ?
-
 ?
1.1.1.430 D-glucose + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia ?
-
 ?
1.1.1.430 D-lyxose + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia ?
-
 ?
1.1.1.430 D-ribose + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia ?
-
 ?
1.1.1.430 D-xylose + NADH + H+ reaction is catalyzed by dual specific xylose reductase (dsXR), reaction is not catalyzed by NADPH-dependent monospecific xylose reductase (msXR) [Candida] intermedia xylitol + NAD+
-
 ?
1.1.1.430 D-xylose + NADPH + H+ reaction is catalyzed by NADPH-dependent monospecific xylose reductase (msXR), and by dual specific xylose reductase (dsXR) [Candida] intermedia xylitol + NADP+
-
 ?
1.1.1.430 DL-glyceraldehyde + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia glycerol + NAD+
-
 ?
1.1.1.430 L-arabinose + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia L-arabinitol + NAD+
-
 ?
1.1.1.430 L-lyxose + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia ?
-
 ?
1.1.1.430 additional information Candida intermedia produces two isoforms of xylose reductase: one is NADPH-dependent (monospecific xylose reductase, msXR, cf. 1.1.1.431), and another prefers NADH about 4fold over NADPH (dual specific xylose reductase, dsXR) [Candida] intermedia ?
-
-
1.1.1.430 pentanal + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia ?
-
 ?
1.1.1.430 propionaldehyde + NADH + H+ dual specific xylose reductase (dsXR) [Candida] intermedia ?
-
 ?
1.1.1.431 2-deoxy-D-galactose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
1.1.1.431 2-deoxy-D-glucose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
1.1.1.431 2-deoxy-D-ribose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
1.1.1.431 butanal + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
1.1.1.431 D-erythrose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
1.1.1.431 D-fucose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
1.1.1.431 D-galactose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
1.1.1.431 D-glucose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
1.1.1.431 D-lyxose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
1.1.1.431 D-ribose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
1.1.1.431 D-xylose + NADH + H+ reaction is catalyzed by dual specific xylose reductase (dsXR), reaction is not catalyzed by NADPH-dependent monospecific xylose reductase (msXR) [Candida] intermedia xylitol + NAD+
-
 ?
1.1.1.431 D-xylose + NADPH + H+
-
 [Candida] intermedia xylitol + NADP+
-
 r
1.1.1.431 D-xylose + NADPH + H+ reaction is catalyzed by NADPH-dependent monospecific xylose reductase (msXR), and by dual specific xylose reductase (dsXR) [Candida] intermedia xylitol + NADP+
-
 ?
1.1.1.431 DL-glyceraldehyde + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia glycerol + NADP+
-
 ?
1.1.1.431 L-arabinose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia L-arabinitol + NADP+
-
 ?
1.1.1.431 L-lyxose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
1.1.1.431 additional information Candida intermedia produces two isoforms of xylose reductase: one is NADPH-dependent (monospecific xylose reductase, msXR), and another prefers NADH about 4fold over NADPH (dual specific xylose reductase, dsXR) [Candida] intermedia ?
-
-
1.1.1.431 pentanal + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
1.1.1.431 propionaldehyde + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
1.1.1.431 xylitol + NADP+
-
 [Candida] intermedia D-xylose + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.307 dsXR
-
 [Candida] intermedia
1.1.1.307 dual specific xylose reductase
-
 [Candida] intermedia
1.1.1.430 dsXR Candida intermedia produces two isoforms of xylose reductase: one is NADPH-dependent (monospecific xylose reductase, msXR, cf. 1.1.1.431), and another prefers NADH about 4fold over NADPH (dual specific xylose reductase, dsXR) [Candida] intermedia
1.1.1.431 monospecific xylose reductase
-
 [Candida] intermedia
1.1.1.431 msXR
-
 [Candida] intermedia
1.1.1.431 msXR Candida intermedia produces two isoforms of xylose reductase: one is NADPH-dependent (monospecific xylose reductase, msXR), and another prefers NADH about 4fold over NADPH (dual specific xylose reductase, dsXR, cf. 1.1.1.430) [Candida] intermedia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.430 25
-
 assay at [Candida] intermedia
1.1.1.431 25
-
 assay at [Candida] intermedia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.307 16
-
 NADH pH 7, 25°C [Candida] intermedia
1.1.1.307 16.3
-
 NADPH pH 7, 25°C [Candida] intermedia
1.1.1.430 3.5
-
 2-deoxy-D-galactose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 4.6
-
 propionaldehyde pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 4.9
-
 D-ribose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 5.4
-
 Butanal pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 5.6
-
 D-glucose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 5.9
-
 pentanal pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 6.6
-
 L-Lyxose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 9.4
-
 D-galactose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 10.2
-
 D-fucose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 13.5
-
 L-arabinose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 14.1
-
 DL-glyceraldehyde pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 16.9
-
 D-xylose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 27.5
-
 D-erythrose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.431 0.6
-
 xylitol pH 7, 25°C [Candida] intermedia
1.1.1.431 1.4
-
 2-deoxy-D-ribose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 2 8 D-xylose pH 7, 25°C [Candida] intermedia
1.1.1.431 6.8
-
 2-deoxy-D-glucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 6.9
-
 propionaldehyde pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 8.2
-
 D-glucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 8.4
-
 2-deoxy-D-galactose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 12.2
-
 D-ribose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 15.2
-
 D-galactose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 18.4
-
 L-Lyxose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 20.7
-
 pentanal pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 20.7
-
 D-fucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 21.2
-
 Butanal pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 23.5
-
 D-xylose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 24.3
-
 DL-glyceraldehyde pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 24.3
-
 D-erythrose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 24.5
-
 L-arabinose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.430 7
-
 assay at [Candida] intermedia
1.1.1.431 7
-
 assay at [Candida] intermedia

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.307 NAD+
-
 [Candida] intermedia
1.1.1.307 NADH
-
 [Candida] intermedia
1.1.1.307 NADP+
-
 [Candida] intermedia
1.1.1.307 NADPH dsXR binds NAD(P)+ weakly and NADH about as tightly as NADPH [Candida] intermedia
1.1.1.430 NADH dual specific xylose reductase (dsXR) has an about 4fold higher specificity for NADH than NADPH [Candida] intermedia
1.1.1.430 NADPH dual specific xylose reductase (dsXR) has an about 4fold higher specificity for NADH than NADPH. NADPH-dependent monospecific xylose reductase (msXR) shows non activity with NADH [Candida] intermedia
1.1.1.431 NADP+
-
 [Candida] intermedia
1.1.1.431 NADPH
-
 [Candida] intermedia
1.1.1.431 NADPH dual specific xylose reductase (dsXR) has an about 4fold higher specificity for NADH than NADPH. NADPH-dependent monospecific xylose reductase (msXR) shows non activity with NADH [Candida] intermedia

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.307 0.0081
-
 NADH pH 7, 25°C [Candida] intermedia
1.1.1.307 0.014
-
 NADPH pH 7, 25°C [Candida] intermedia
1.1.1.307 0.053
-
 NADP+ pH 7, 25°C [Candida] intermedia
1.1.1.307 0.074
-
 NAD+ pH 7, 25°C [Candida] intermedia
1.1.1.430 0.008
-
 NADH pH 7.0, 25°C, dual specific xylose reductase (dsXR) [Candida] intermedia
1.1.1.430 0.014
-
 NADPH pH 7.0, 25°C, dual specific xylose reductase (dsXR) [Candida] intermedia
1.1.1.430 0.053
-
 NADP+ pH 7.0, 25°C, dual specific xylose reductase (dsXR) [Candida] intermedia
1.1.1.430 0.074
-
 NAD+ pH 7.0, 25°C, dual specific xylose reductase (dsXR) [Candida] intermedia
1.1.1.431 0.02
-
 NADPH pH 7, 25°C [Candida] intermedia
1.1.1.431 0.02
-
 NADPH pH 7.0, 25°C, monospecific xylose reductase (msXR) [Candida] intermedia
1.1.1.431 0.025
-
 NADP+ pH 7, 25°C [Candida] intermedia
1.1.1.431 0.025
-
 NADP+ pH 7.0, 25°C, monospecific xylose reductase (msXR) [Candida] intermedia

General Information

EC Number General Information Comment Organism
1.1.1.307 metabolism ordered mechanism in which coenzyme binds first and substrate second [Candida] intermedia
1.1.1.431 metabolism ordered mechanism in which coenzyme binds first and substrate second [Candida] intermedia

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.307 0.005
-
 xylitol cosubstrate NADP+, pH 7, 25°C [Candida] intermedia
1.1.1.307 0.023
-
 xylitol cosubstrate NAD+, pH 7, 25°C [Candida] intermedia
1.1.1.307 0.25
-
 D-xylose cosubstrate NADPH, pH 7, 25°C [Candida] intermedia
1.1.1.307 0.53
-
 D-xylose cosubstrate NADH, pH 7, 25°C [Candida] intermedia
1.1.1.430 0.035
-
 propionaldehyde pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 0.16
-
 Butanal pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 0.4
-
 pentanal pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 5.8
-
 DL-glyceraldehyde pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 28
-
 2-deoxy-D-galactose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 46
-
 L-Lyxose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 72
-
 D-ribose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 170
-
 D-glucose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 482
-
 L-arabinose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 627
-
 D-galactose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 1380
-
 D-erythrose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 1457
-
 D-fucose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.430 1690
-
 D-xylose pH 7.0, 25°C, dual specific xylose reductase, cofactor: NADH [Candida] intermedia
1.1.1.431 0.0013
-
 xylitol pH 7, 25°C [Candida] intermedia
1.1.1.431 0.09
-
 propionaldehyde pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 0.36
-
 D-xylose pH 7, 25°C [Candida] intermedia
1.1.1.431 0.93
-
 Butanal pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 5.31
-
 pentanal pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 21.3
-
 DL-glyceraldehyde pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 36.8
-
 2-deoxy-D-ribose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 38
-
 2-deoxy-D-galactose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 117
-
 2-deoxy-D-glucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 134
-
 D-ribose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 157
-
 L-Lyxose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 249
-
 D-galactose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 736
-
 D-erythrose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 1230
-
 L-arabinose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 1370
-
 D-glucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 1470
-
 D-xylose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.1.1.431 2070
-
 D-fucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia