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Literature summary extracted from

  • An, J.U.; Lee, I.G.; Ko, Y.J.; Oh, D.K.
    Microbial synthesis of linoleate 9S-lipoxygenase derived plant C18 oxylipins from C18 polyunsaturated fatty acids (2019), J. Agric. Food Chem., 67, 3209-3219 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.58 expressed in Escherichia coli ER2566 cells Myxococcus xanthus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.58 0.073
-
gamma-linolenic acid at pH 8.5 and 35°C Myxococcus xanthus
1.13.11.58 0.081
-
alpha-linolenic acid at pH 8.5 and 35°C Myxococcus xanthus
1.13.11.58 0.33
-
linoleic acid at pH 8.5 and 35°C Myxococcus xanthus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.58 Myxococcus xanthus
-
-
-
1.13.11.58 Myxococcus xanthus DK1622
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.58 His-Trap affinity chromatography Myxococcus xanthus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.58 12-hydroperoxy-cis,trans,cis-9,13,15-octadecatrienoic acid + O2
-
Myxococcus xanthus 12,13-epoxy-14-hydroxy-cis,cis-9,15-octadecadienoic acid
-
?
1.13.11.58 12-hydroperoxy-cis,trans,cis-9,13,15-octadecatrienoic acid + O2
-
Myxococcus xanthus DK1622 12,13-epoxy-14-hydroxy-cis,cis-9,15-octadecadienoic acid
-
?
1.13.11.58 9-hydroperoxy-cis,trans,cis-6,10,12-octadecatrienoic acid + O2
-
Myxococcus xanthus 9,10-epoxy-11-hydroxy-cis,cis-6,12-octadecadienoic acid
-
?
1.13.11.58 9-hydroperoxy-cis,trans,cis-6,10,12-octadecatrienoic acid + O2
-
Myxococcus xanthus DK1622 9,10-epoxy-11-hydroxy-cis,cis-6,12-octadecadienoic acid
-
?
1.13.11.58 9-hydroperoxy-trans,cis-10,12-octadecadienoic acid + O2
-
Myxococcus xanthus 9,10-epoxy-11-hydroxy-cis-12-octadecenoic acid
-
?
1.13.11.58 9-hydroperoxy-trans,cis-10,12-octadecadienoic acid + O2
-
Myxococcus xanthus DK1622 9,10-epoxy-11-hydroxy-cis-12-octadecenoic acid
-
?
1.13.11.58 alpha-linolenic acid + O2
-
Myxococcus xanthus 12-hydroperoxy-cis,trans,cis-9,13,15-octadecatrienoic acid
-
?
1.13.11.58 alpha-linolenic acid + O2
-
Myxococcus xanthus DK1622 12-hydroperoxy-cis,trans,cis-9,13,15-octadecatrienoic acid
-
?
1.13.11.58 gamma-linoleic acid + O2
-
Myxococcus xanthus 9-hydroperoxy-cis,trans,cis-6,10,12-octadecatrienoic acid
-
?
1.13.11.58 gamma-linoleic acid + O2
-
Myxococcus xanthus DK1622 9-hydroperoxy-cis,trans,cis-6,10,12-octadecatrienoic acid
-
?
1.13.11.58 gamma-linolenic acid + O2
-
Myxococcus xanthus 9 9-hydroperoxy-cis,trans,cis-6,10,12-octadecatrienoic acid
-
?
1.13.11.58 linoleic acid + O2
-
Myxococcus xanthus 9-hydroperoxy-trans,cis-10,12-octadecadienoic acid
-
?
1.13.11.58 linolenic acid + O2
-
Myxococcus xanthus 9-hydroperoxy-trans,cis-10,12-octadecadienoic acid
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.58 ? x * 76000, His-tagged enzyme, SDS-PAGE Myxococcus xanthus

Synonyms

EC Number Synonyms Comment Organism
1.13.11.58 linoleate 9S-lipoxygenase
-
Myxococcus xanthus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.58 0.81
-
gamma-linolenic acid at pH 8.5 and 35°C Myxococcus xanthus
1.13.11.58 1.27
-
alpha-linolenic acid at pH 8.5 and 35°C Myxococcus xanthus
1.13.11.58 7.95
-
linoleic acid at pH 8.5 and 35°C Myxococcus xanthus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.58 11.05
-
gamma-linolenic acid at pH 8.5 and 35°C Myxococcus xanthus
1.13.11.58 15.7
-
alpha-linolenic acid at pH 8.5 and 35°C Myxococcus xanthus
1.13.11.58 24.1
-
linoleic acid at pH 8.5 and 35°C Myxococcus xanthus