Literature summary extracted from

Liu, B.; Peng, Q.; Sheng, M.; Ni, H.; Xiao, X.; Tao, Q.; He, Q.; He, J.
Isolation and characterization of a topramezone-resistant 4-hydroxyphenylpyruvate dioxygenase from Sphingobium sp. TPM-19 (2020), J. Agric. Food Chem., 68, 1022-1029 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.13.11.27	expression in Escherichia coli	Sphingobium sp.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.13.11.27	DEPC	1 mM, no residual activity	Sphingobium sp.
1.13.11.27	EDTA	1 mM, no residual activity	Sphingobium sp.
1.13.11.27	isoxaflutole	-	Sphingobium sp.
1.13.11.27	mesotrione	-	Sphingobium sp.
1.13.11.27	additional information	not inhibitory at 1 mM: urea, PMSF	Sphingobium sp.
1.13.11.27	tembotrione	-	Sphingobium sp.
1.13.11.27	topramezone	-	Sphingobium sp.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.13.11.27	0.083	-	4-hydroxyphenylpyruvate	pH 6, 35°C	Sphingobium sp.

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.13.11.27	Ca2+	66.5% of the activity with Fe2+	Sphingobium sp.
1.13.11.27	Fe2+	required	Sphingobium sp.
1.13.11.27	Fe3+	95% of the reactivity with Fe2+	Sphingobium sp.
1.13.11.27	Mg2+	101% of the activity with Fe2+	Sphingobium sp.
1.13.11.27	Mn2+	22% of the activity with Fe2+	Sphingobium sp.

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.13.11.27	40000	-	gel filtration	Sphingobium sp.

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.13.11.27	Sphingobium sp.	A0A6B9L0W0	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.13.11.27	recombinant enzyme, using Co2+-affinity chromatography	Sphingobium sp.

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.13.11.27	4-hydroxyphenylpyruvate + O2	-	Sphingobium sp.	homogentisate + CO2	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.13.11.27	monomer	1 * 41000, SDS-PAGE, 1 * 38800, calculated from sequence	Sphingobium sp.

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.13.11.27	15	-	4-hydroxyphenylpyruvate	pH 6, 35°C	Sphingobium sp.

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.13.11.27	0.001	-	tembotrione	pH 6, 35°C	Sphingobium sp.
1.13.11.27	0.0013	-	mesotrione	pH 6, 35°C	Sphingobium sp.
1.13.11.27	0.0025	-	topramezone	pH 6, 35°C	Sphingobium sp.
1.13.11.27	0.006	-	isoxaflutole	pH 6, 35°C	Sphingobium sp.

IC50 Value

EC Number	IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
1.13.11.27	0.0025	-	pH 6, 35°C	Sphingobium sp.	tembotrione
1.13.11.27	0.0042	-	pH 6, 35°C	Sphingobium sp.	mesotrione
1.13.11.27	0.0052	-	pH 6, 35°C	Sphingobium sp.	topramezone
1.13.11.27	0.0087	-	pH 6, 35°C	Sphingobium sp.	isoxaflutole

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.13.11.27	181.2	-	4-hydroxyphenylpyruvate	pH 6, 35°C	Sphingobium sp.

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Release 2023.1 (January 2023)