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Literature summary extracted from
- Production of 11R-hydroxyeicosatetraenoic acid from arachidonic acid by Escherichia coli cells expressing arachidonate 11R-lipoxygenase from Nostoc sp. (2021), J. Am. Oil Chem. Soc., 2021, 1-9 .
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.61 | DMSO | about 145% activity at 5% (v/v) | Nostoc sp. PCC 7120 = FACHB-418 |  |
| 1.13.11.61 | ethanol | about 155% activity at 5% (v/v) | Nostoc sp. PCC 7120 = FACHB-418 | |
| 1.13.11.61 | iso-propanol | about 140% activity at 5% (v/v) | Nostoc sp. PCC 7120 = FACHB-418 | |
| 1.13.11.61 | methanol | about 130% activity at 5% (v/v) | Nostoc sp. PCC 7120 = FACHB-418 | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.11.61 | expressed in Escherichia coli ER2566 cells | Nostoc sp. PCC 7120 = FACHB-418 |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.61 | acetone | about 60% residual activity at 2% (v/v) | Nostoc sp. PCC 7120 = FACHB-418 | |
| 1.13.11.61 | butanol | about 65% residual activity at 5% (v/v) | Nostoc sp. PCC 7120 = FACHB-418 | |
| 1.13.11.61 | ethyl acetate | about 70% residual activity at 5% (v/v) | Nostoc sp. PCC 7120 = FACHB-418 | |
| 1.13.11.61 | hexane | about 80% residual activity at 2% (v/v) | Nostoc sp. PCC 7120 = FACHB-418 | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.61 | 0.054 | - |
alpha-linolenic acid | at pH 7.0 and 25°C | Nostoc sp. PCC 7120 = FACHB-418 | |
| 1.13.11.61 | 0.054 | - |
gamma-linolenic acid | at pH 7.0 and 25°C | Nostoc sp. PCC 7120 = FACHB-418 | |
| 1.13.11.61 | 0.404 | - |
linoleic acid | at pH 7.0 and 25°C | Nostoc sp. PCC 7120 = FACHB-418 | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.61 | Nostoc sp. PCC 7120 = FACHB-418 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.61 | His-Trap column chromatography and Superdex 200 gel filtration | Nostoc sp. PCC 7120 = FACHB-418 |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.61 | 5.4 | - |
native enzyme, with gamma-linolenic acid as substrate, at pH 7.0 and 25°C | Nostoc sp. PCC 7120 = FACHB-418 |
| 1.13.11.61 | 6.3 | - |
native enzyme, with alpha-linolenic acid as substrate, at pH 7.0 and 25°C | Nostoc sp. PCC 7120 = FACHB-418 |
| 1.13.11.61 | 18.4 | - |
native enzyme, with linoleic acid as substrate, at pH 7.0 and 25°C | Nostoc sp. PCC 7120 = FACHB-418 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.61 | alpha-linolenic acid + O2 | - |
Nostoc sp. PCC 7120 = FACHB-418 | (9Z,12E,15Z)-9R-hydroxy-9,12,15-octadecatrienoic acid | - |
? | |
| 1.13.11.61 | gamma-linolenic acid + O2 | - |
Nostoc sp. PCC 7120 = FACHB-418 | (6Z,10E,12Z)-9R-hydroxy-6,10,12-octadecatrienoic acid | - |
? | |
| 1.13.11.61 | linoleic acid + O2 | - |
Nostoc sp. PCC 7120 = FACHB-418 | (10E,12Z)-9R-hydroxy-10,12-octadecadienoic acid | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.61 | 11R-LOX | - |
Nostoc sp. PCC 7120 = FACHB-418 |
| 1.13.11.61 | 9R-LOX | - |
Nostoc sp. PCC 7120 = FACHB-418 |
| 1.13.11.61 | ARA 11RLOX | - |
Nostoc sp. PCC 7120 = FACHB-418 |
| 1.13.11.61 | arachidonate 11R-lipoxygenase | - |
Nostoc sp. PCC 7120 = FACHB-418 |
| 1.13.11.61 | LA 9RLOX | - |
Nostoc sp. PCC 7120 = FACHB-418 |
| 1.13.11.61 | linoleate 11R-lipoxygenase | - |
Nostoc sp. PCC 7120 = FACHB-418 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.61 | 25 | - |
- |
Nostoc sp. PCC 7120 = FACHB-418 |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.61 | 15 | 35 | more than 60% activity between 15 and 35°C | Nostoc sp. PCC 7120 = FACHB-418 |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.61 | 0.097 | - |
gamma-linolenic acid | at pH 7.0 and 25°C | Nostoc sp. PCC 7120 = FACHB-418 | |
| 1.13.11.61 | 0.13 | - |
alpha-linolenic acid | at pH 7.0 and 25°C | Nostoc sp. PCC 7120 = FACHB-418 | |
| 1.13.11.61 | 5.12 | - |
linoleic acid | at pH 7.0 and 25°C | Nostoc sp. PCC 7120 = FACHB-418 | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.61 | 7 | - |
- |
Nostoc sp. PCC 7120 = FACHB-418 |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.61 | 6.5 | 8.5 | more than 50% activity between pH 6.5 and 8.5 | Nostoc sp. PCC 7120 = FACHB-418 |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.61 | 1.8 | - |
gamma-linolenic acid | at pH 7.0 and 25°C | Nostoc sp. PCC 7120 = FACHB-418 | |
| 1.13.11.61 | 2.4 | - |
alpha-linolenic acid | at pH 7.0 and 25°C | Nostoc sp. PCC 7120 = FACHB-418 | |
| 1.13.11.61 | 12.7 | - |
linoleic acid | at pH 7.0 and 25°C | Nostoc sp. PCC 7120 = FACHB-418 | |
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