Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Naidu, D.; Ragsdale, S.
    Characterization of a three-component vanillate O-demethylase from Moorella thermoacetica (2001), J. Bacteriol., 183, 3276-3281 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.382 O2
-
Moorella thermoacetica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.382 0.083
-
vanillate pH 6.6, 55°C Moorella thermoacetica
2.1.1.382 9
-
dicamba pH 6.6, 55°C Moorella thermoacetica
2.1.1.385 0.23
-
tetrahydrofolate pH 6.6, 55°C Moorella thermoacetica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.382 Co(I) reaction involves Co(I), and is O2 sensitive Moorella thermoacetica
2.1.1.385 additional information no stimulation by Zn2+ up to 4 mM Moorella thermoacetica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.385 48000
-
gel filtration Moorella thermoacetica

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.382 Moorella thermoacetica
-
-
-
2.1.1.385 Moorella thermoacetica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.385
-
Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.382 dicamba + [Co(I) methoxylated corrinoid protein MtvC]
-
Moorella thermoacetica a [methyl-Co(III) methoxylated corrinoid protein MtvC] + 3,6-dichloro-2-hydroxybenzoate
-
?
2.1.1.382 vanillate + [Co(I) methoxylated corrinoid protein MtvC]
-
Moorella thermoacetica a [methyl-Co(III) methoxylated corrinoid protein MtvC] + 3,4-dihydroxybenzoate
-
?
2.1.1.385 additional information MtvA shows no activity in the vanillate- or dicamba-dependent methylation of cob(I)alamin, reaction of EC 2.1.1.382 Moorella thermoacetica ?
-
-
2.1.1.385 [methyl-Co(III) methoxylated-aromatic-compound-specific corrinoid protein MtvC] + tetrahydrofolate
-
Moorella thermoacetica N5-methyltetrahydrofolate + [Co(I) methoxylated-aromatic-compound-specific corrinoid protein MtvC]
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.382 ? x * 48000, SDS-PAGE Moorella thermoacetica
2.1.1.385 dimer 2 29500, SDS-PAGE Moorella thermoacetica

Synonyms

EC Number Synonyms Comment Organism
2.1.1.382 mtvB
-
Moorella thermoacetica
2.1.1.385 mtvA
-
Moorella thermoacetica

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.382 tetrahydrofolate required as the acceptor of the methyl group from vanillate, Km value 0.23 mM Moorella thermoacetica

General Information

EC Number General Information Comment Organism
2.1.1.382 physiological function MtvB is part of an inducible three-component aromatic O-demethylase system. MtvB catalyzes methyl transfer from a phenylmethylether to the cobalt center of MtvC, a corrinoid protein. All three protein components are required for O-demethylase activity Moorella thermoacetica
2.1.1.385 physiological function MtvA is part of an inducible three-component aromatic O-demethylase system. MtvA catalyzes transmethylation from MtvC to tetrahydrofolate, forming methyltetrahydrofolate. All three protein components are required for O-demethylase activity Moorella thermoacetica