EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.382 | O2 | - |
Moorella thermoacetica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.382 | 0.083 | - |
vanillate | pH 6.6, 55°C | Moorella thermoacetica | |
2.1.1.382 | 9 | - |
dicamba | pH 6.6, 55°C | Moorella thermoacetica | |
2.1.1.385 | 0.23 | - |
tetrahydrofolate | pH 6.6, 55°C | Moorella thermoacetica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.382 | Co(I) | reaction involves Co(I), and is O2 sensitive | Moorella thermoacetica | |
2.1.1.385 | additional information | no stimulation by Zn2+ up to 4 mM | Moorella thermoacetica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.385 | 48000 | - |
gel filtration | Moorella thermoacetica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.382 | Moorella thermoacetica | - |
- |
- |
2.1.1.385 | Moorella thermoacetica | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.385 | - |
Moorella thermoacetica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.382 | dicamba + [Co(I) methoxylated corrinoid protein MtvC] | - |
Moorella thermoacetica | a [methyl-Co(III) methoxylated corrinoid protein MtvC] + 3,6-dichloro-2-hydroxybenzoate | - |
? | |
2.1.1.382 | vanillate + [Co(I) methoxylated corrinoid protein MtvC] | - |
Moorella thermoacetica | a [methyl-Co(III) methoxylated corrinoid protein MtvC] + 3,4-dihydroxybenzoate | - |
? | |
2.1.1.385 | additional information | MtvA shows no activity in the vanillate- or dicamba-dependent methylation of cob(I)alamin, reaction of EC 2.1.1.382 | Moorella thermoacetica | ? | - |
- |
|
2.1.1.385 | [methyl-Co(III) methoxylated-aromatic-compound-specific corrinoid protein MtvC] + tetrahydrofolate | - |
Moorella thermoacetica | N5-methyltetrahydrofolate + [Co(I) methoxylated-aromatic-compound-specific corrinoid protein MtvC] | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.382 | ? | x * 48000, SDS-PAGE | Moorella thermoacetica |
2.1.1.385 | dimer | 2 29500, SDS-PAGE | Moorella thermoacetica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.382 | mtvB | - |
Moorella thermoacetica |
2.1.1.385 | mtvA | - |
Moorella thermoacetica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.382 | tetrahydrofolate | required as the acceptor of the methyl group from vanillate, Km value 0.23 mM | Moorella thermoacetica |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.382 | physiological function | MtvB is part of an inducible three-component aromatic O-demethylase system. MtvB catalyzes methyl transfer from a phenylmethylether to the cobalt center of MtvC, a corrinoid protein. All three protein components are required for O-demethylase activity | Moorella thermoacetica |
2.1.1.385 | physiological function | MtvA is part of an inducible three-component aromatic O-demethylase system. MtvA catalyzes transmethylation from MtvC to tetrahydrofolate, forming methyltetrahydrofolate. All three protein components are required for O-demethylase activity | Moorella thermoacetica |